Adaption of Sprague Dawley rats to long-term feeding of high fat of high fructose diets

2000 ◽  
Vol 39 (5) ◽  
pp. 229-234 ◽  
Author(s):  
A.H. Stark ◽  
B. Timar ◽  
Z. Madar
Keyword(s):  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
High Fructose

scholarly journals High-Fat-High-Fructose Diet Decreases Hippocampal Neuron Number in Male Rats

2020 ◽  
Vol 12 (1) ◽  
pp. 1-7
Author(s):  
Inggita Kusumastuty ◽  
Frinny Sembiring ◽  
Sri Andarini ◽  
Dian Handayani
Keyword(s):  
Cell Death ◽  
Nerve Cell ◽  
Hippocampal Neurons ◽  
Sprague Dawley ◽  
High Fat ◽  
Group I ◽  
Sprague Dawley Rats ◽  
High Fructose ◽  
Group Ii ◽  
Nerve Cell Death

BACKGROUND: Consumption of foods and drinks high in energy, fat, and/or sugar beyond the recommended quantities can cause obesity, which triggers the incidence of brain nerve cell death related to oxidative stress, high levels of tumor necrosis factor (TNF)-α and triglycerides, and low high-density lipoprotein (HDL) levels. Progressive nerve cell death causes decreasing cognitive performance. This study aims to prove that an American Institute of Nutrition committee in 1993 (AIN-93M) diet modified with high-fat-high-fructose (HFHF) can decrease the number of hippocampal neurons. A decrease in the number of hippocampal neurons indicates progressive nerve cell death.METHODS: An experimental study using a post-test control group design was carried out using male Sprague Dawley rats. Samples were selected using simple random sampling to divide them into two groups, Group I was AIN-93M-modified HFHF diet (n=14) and Group II was AIN-93M standard (n=16). The number of visible neurons was measured in the hippocampus area of Sprague Dawley rats’ brains, stained with haemotoxylin and eosin (H&E) and scanned under 400x magnification. Neurons were counted in 10 visual fields using the "Cell_Count" application.RESULTS: The data were analysed by Pearson’s correlation test using SPSS. The results show that rats in Group I had a greater weight gain and fewer neurons than those in the Group II (p=0.023, r=-0.413).CONCLUSION: The consumption of foods high in fat and fructose can cause an increase in nerve cell death, as shown by the decrease in the number of hippocampal neurons.KEYWORDS: brain nerve cells, high fat, high fructose, increased body weight

Effects of a Long-Term High-Fat Diet and Switching from a High-Fat to Low-Fat, Standard Diet on Hepatic Fat Accumulation in Sprague-Dawley Rats

2008 ◽  
Vol 53 (12) ◽  
pp. 3206-3212 ◽  
Author(s):  
Katsuhisa Omagari ◽  
Shigeko Kato ◽  
Koichi Tsuneyama ◽  
Chisato Inohara ◽  
Yu Kuroda ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Standard Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Low Fat ◽  
Fat Accumulation ◽  
Sprague Dawley Rats ◽  
Hepatic Fat

Leptin secretion after a high-fat meal in normal-weight rats: strong predictor of long-term body fat accrual on a high-fat diet

2006 ◽  
Vol 290 (2) ◽  
pp. E258-E267 ◽  
Author(s):  
S. F. Leibowitz ◽  
G.-Q. Chang ◽  
J. T. Dourmashkin ◽  
R. Yun ◽  
C. Julien ◽  
...  
Keyword(s):  
Body Fat ◽  
High Fat Diet ◽  
Strong Predictor ◽  
Normal Weight ◽  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Orexigenic Peptide ◽  
Fat Meal

The objective of this study was to investigate meal-related endocrine changes that permit one to identify Sprague-Dawley rats at normal weight that are prone (OP) vs. resistant (OR) to obesity. In blood collected via chronic cardiac catheters, a 2-h high-fat meal (HFM, 50% fat, 40 kcal) at dark onset caused a significant increase in leptin, insulin, and triglycerides compared with premeal levels. Similar to patterns in already obese compared with lean rats on a high-fat diet, these meal-induced endocrine changes in normal-weight rats on lab chow were almost twofold larger in OP rats that, compared with OR rats, subsequently accumulated 100% more fat mass on a chronic high-fat diet. These exaggerated endocrine changes were similarly observed in blood collected using a simpler tail vein puncture procedure. In three separate experiments, the HFM-induced rise in leptin was found to be the strongest, positive correlate ( r = +0.58, +0.62 and +0.64) of long-term body fat accrual. The lowest (2–5 ng/ml) vs. highest (6–9 ng/ml) scores for this post-HFM leptin measurement identified distinct OR and OP subgroups, respectively, when they were similar in body weight (340–350 g), premeal leptin (2.6–3.4 ng/ml), and meal size (40 kcal). Subsequent tests in these normal-weight OP rats revealed a distinct characteristic compared with OR rats, namely, exaggerated HFM-induced rise in expression of the orexigenic peptide galanin in the paraventricular nucleus. Thus, with this HFM-induced leptin measurement, OP rats can be identified while still at normal weight and then investigated for mechanisms that contribute to their excessive body fat accrual on a high-fat diet.

Effects of long-term consumption of a high-fructose diet on conventional cardiovascular risk factors in Sprague-Dawley rats

2009 ◽  
Vol 327 (1-2) ◽  
pp. 247-256 ◽  
Author(s):  
Mohammad M. Abdullah ◽  
Natalie N. Riediger ◽  
Qilin Chen ◽  
Zhaohui Zhao ◽  
Nazila Azordegan ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cardiovascular Risk ◽  
Cardiovascular Risk Factors ◽  
Sprague Dawley ◽  
High Fructose Diet ◽  
Sprague Dawley Rats ◽  
High Fructose

scholarly journals Fructose-induced leptin resistance exacerbates weight gain in response to subsequent high-fat feeding

2008 ◽  
Vol 295 (5) ◽  
pp. R1370-R1375 ◽  
Author(s):  
Alexandra Shapiro ◽  
Wei Mu ◽  
Carlos Roncal ◽  
Kit-Yan Cheng ◽  
Richard J. Johnson ◽  
...  
Keyword(s):  
Weight Gain ◽  
High Fat Diet ◽  
Leptin Resistance ◽  
Sprague Dawley ◽  
High Fat ◽  
Serum Leptin ◽  
Fructose Intake ◽  
Sprague Dawley Rats ◽  
High Fructose ◽  
Subsequent Exposure

It has been suggested that increased fructose intake is associated with obesity. We hypothesized that chronic fructose consumption causes leptin resistance, which subsequently may promote the development of obesity in response to a high-fat diet. Sprague-Dawley rats were fed a fructose-free control or 60% fructose diet for 6 mo and then tested for leptin resistance. Half of the rats in each group were then switched to high-fat diet for 2 wk, while the other half continued on their respective diets. Chronic fructose consumption caused leptin resistance, while serum leptin levels, weight, and adiposity were the same as in control rats that were leptin responsive. Intraperitoneal leptin injections reduced 24-h food intake in the fructose-free group (73.7 ± 6.3 vs. 58.1 ± 8 kcal, P = 0.02) but had no effect in fructose-fed rats (71.2 ± 6.6 vs. 72.4 ± 6.4 kcal, P = 0.9). Absence of anorexic response to intraperitoneal leptin injection was associated with 25.7% decrease in hypothalamic signal transducer and activator of transcription 3 phosphorylation in the high-fructose-fed rats compared with controls ( P = 0.015). Subsequent exposure of the fructose-mediated, leptin-resistant rats to a high-fat diet led to exacerbated weight gain (50.2 ± 2 g) compared with correspondingly fed leptin-responsive animals that were pretreated with the fructose-free diet (30.4 ± 5.8 g, P = 0.012). Our data indicate that chronic fructose consumption induces leptin resistance prior to body weight, adiposity, serum leptin, insulin, or glucose increases, and this fructose-induced leptin resistance accelerates high-fat induced obesity.

scholarly journals Effects of Dietary Brown Rice on Carcass Composition and Nitric Oxide (NOx) Metabolite Levels in High-Fat High-Fructose Diet-induced Sprague Dawley Rats as Obesity Model

2020 ◽  
Vol 1665 ◽  
pp. 012007
Author(s):  
Dian Handayani ◽  
Ahmad Ramadhan ◽  
Risma Debby Anindyanti ◽  
Alma Maghfirotun Innayah ◽  
Etik Sulistyowati ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Brown Rice ◽  
Carcass Composition ◽  
Sprague Dawley ◽  
High Fat ◽  
High Fructose Diet ◽  
Sprague Dawley Rats ◽  
High Fructose

scholarly journals Effects of long-term consumption of high fructose corn syrup containing peach nectar on body weight gain in sprague dawley rats

2017 ◽  
Vol 37 (2) ◽  
pp. 337-343
Author(s):  
Gulsah OZCAN SINIR ◽  
Senem SUNA ◽  
Sevda INAN ◽  
Deniz BAGDAS ◽  
Canan Ece TAMER ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
High Fructose Corn Syrup ◽  
Sprague Dawley ◽  
Corn Syrup ◽  
Sprague Dawley Rats ◽  
High Fructose
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