Molecular characterization and sequencing of antifreeze proteins from larvae of the beetle Dendroides canadensis

1998 ◽  
Vol 168 (3) ◽  
pp. 225-232 ◽  
Author(s):  
J. G. Duman ◽  
N. Li ◽  
D. Verleye ◽  
F. W. Goetz ◽  
D. W. Wu ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Antifreeze Proteins ◽  
Dendroides Canadensis

scholarly journals Recombinant Dendroides canadensis antifreeze proteins as potential ingredients in cryopreservation solutions

Cryobiology ◽  
2014 ◽  
Vol 68 (3) ◽  
pp. 411-418 ◽  
Author(s):  
Dina O. Halwani ◽  
Kelvin G.M. Brockbank ◽  
John G. Duman ◽  
Lia H. Campbell
Keyword(s):  
Antifreeze Proteins ◽  
Dendroides Canadensis

Purification and characterization of antifreeze proteins from larvae of the beetle Dendroides canadensis

1991 ◽  
Vol 161 (3) ◽  
pp. 271-278 ◽  
Author(s):  
Ding W. Wu ◽  
John G. Duman ◽  
Chi-Hing C. Cheng ◽  
Francis J. Castellino
Keyword(s):  
Antifreeze Proteins ◽  
Purification And Characterization ◽  
Dendroides Canadensis

scholarly journals Antifreeze proteins in the primary urine of larvae of the beetle Dendroides canadensis

2013 ◽  
Vol 216 (9) ◽  
pp. 1695-1703 ◽  
Author(s):  
P. K. Nickell ◽  
S. Sass ◽  
D. Verleye ◽  
E. M. Blumenthal ◽  
J. G. Duman
Keyword(s):  
Antifreeze Proteins ◽  
Dendroides Canadensis

scholarly journals Antifreeze proteins govern the precipitation of trehalose in a freezing-avoiding insect at low temperature

2016 ◽  
Vol 113 (24) ◽  
pp. 6683-6688 ◽  
Author(s):  
Xin Wen ◽  
Sen Wang ◽  
John G. Duman ◽  
Josh Fnu Arifin ◽  
Vonny Juwita ◽  
...  
Keyword(s):  
Antifreeze Proteins ◽  
Extreme Environments ◽  
Freezing Temperature ◽  
Industrial Applications ◽  
Dendroides Canadensis ◽  
Crystallization Inhibition ◽  
Novel Approach ◽  
Larval Hemolymph ◽  
Freeze Tolerant ◽  
Dynamics Simulations

The remarkable adaptive strategies of insects to extreme environments are linked to the biochemical compounds in their body fluids. Trehalose, a versatile sugar molecule, can accumulate to high levels in freeze-tolerant and freeze-avoiding insects, functioning as a cryoprotectant and a supercooling agent. Antifreeze proteins (AFPs), known to protect organisms from freezing by lowering the freezing temperature and deferring the growth of ice, are present at high levels in some freeze-avoiding insects in winter, and yet, paradoxically are found in some freeze-tolerant insects. Here, we report a previously unidentified role for AFPs in effectively inhibiting trehalose precipitation in the hemolymph (or blood) of overwintering beetle larvae. We determine the trehalose level (29.6 ± 0.6 mg/mL) in the larval hemolymph of a beetle, Dendroides canadensis, and demonstrate that the hemolymph AFPs are crucial for inhibiting trehalose crystallization, whereas the presence of trehalose also enhances the antifreeze activity of AFPs. To dissect the molecular mechanism, we examine the molecular recognition between AFP and trehalose crystal interfaces using molecular dynamics simulations. The theory corroborates the experiments and shows preferential strong binding of the AFP to the fast growing surfaces of the sugar crystal. This newly uncovered role for AFPs may help explain the long-speculated role of AFPs in freeze-tolerant species. We propose that the presence of high levels of molecules important for survival but prone to precipitation in poikilotherms (their body temperature can vary considerably) needs a companion mechanism to prevent the precipitation and here present, to our knowledge, the first example. Such a combination of trehalose and AFPs also provides a novel approach for cold protection and for trehalose crystallization inhibition in industrial applications.

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