Maintenance of the supercooled state in overwintering pyrochroid beetle larvae, Dendroides canadensis  : role of hemolymph ice nucleators and antifreeze proteins

1997 ◽  
Vol 167 (2) ◽  
pp. 105-113 ◽  
Author(s):  
T. M. Olsen ◽  
J. G. Duman
Keyword(s):  
Antifreeze Proteins ◽  
Dendroides Canadensis ◽  
Supercooled State ◽  
Ice Nucleators

Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation

2018 ◽  
Vol 44 (6) ◽  
pp. 1527-1533 ◽  
Author(s):  
Miaomiao Xin ◽  
Jan Sterba ◽  
Anna Shaliutina-Kolesova ◽  
Borys Dzyuba ◽  
Jaroslava Lieskovska ◽  
...  
Keyword(s):  
Antifreeze Proteins ◽  
Protective Role ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus

Hydration Shell of Antifreeze Proteins: Unveiling the Role of Non-Ice-Binding Surfaces

2019 ◽  
Vol 123 (30) ◽  
pp. 6474-6480 ◽  
Author(s):  
Laura Zanetti-Polzi ◽  
Akash Deep Biswas ◽  
Sara Del Galdo ◽  
Vincenzo Barone ◽  
Isabella Daidone
Keyword(s):  
Antifreeze Proteins ◽  
Hydration Shell ◽  
Ice Binding

Role of the Solvation Water in Remote Interactions of Hyperactive Antifreeze Proteins with the Surface of Ice

2019 ◽  
Vol 123 (38) ◽  
pp. 8010-8018 ◽  
Author(s):  
Joanna Grabowska ◽  
Anna Kuffel ◽  
Jan Zielkiewicz
Keyword(s):  
Antifreeze Proteins

scholarly journals Recombinant Dendroides canadensis antifreeze proteins as potential ingredients in cryopreservation solutions

Cryobiology ◽  
2014 ◽  
Vol 68 (3) ◽  
pp. 411-418 ◽  
Author(s):  
Dina O. Halwani ◽  
Kelvin G.M. Brockbank ◽  
John G. Duman ◽  
Lia H. Campbell
Keyword(s):  
Antifreeze Proteins ◽  
Dendroides Canadensis

Molecular characterization and sequencing of antifreeze proteins from larvae of the beetle Dendroides canadensis

1998 ◽  
Vol 168 (3) ◽  
pp. 225-232 ◽  
Author(s):  
J. G. Duman ◽  
N. Li ◽  
D. Verleye ◽  
F. W. Goetz ◽  
D. W. Wu ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Antifreeze Proteins ◽  
Dendroides Canadensis

Factors contributing to seasonal increases in inoculative freezing resistance in overwintering fire-colored beetle larvae dendroides canadensis

1998 ◽  
Vol 201 (10) ◽  
pp. 1585-1594 ◽  
Author(s):  
T Olsen ◽  
S Sass ◽  
N Li ◽  
J Duman
Keyword(s):  
Epicuticular Waxes ◽  
Freezing Resistance ◽  
Dendroides Canadensis ◽  
Ice Nucleators ◽  
Inoculative Freezing ◽  
Cold Hardy ◽  
Chamber Studies ◽  
Epicuticular Lipids

The insects and microarthropods that vary seasonally in susceptibility to cross-cuticular inoculation by external ice (inoculative freezing) represent a phylogenetically diverse group; however, few studies have explored possible mechanisms experimentally. This study documents seasonally variable inoculative freezing resistance in Dendroides canadensis beetle larvae and combines immunofluorescence, in vivo removal of epicuticular lipids and in vitro chamber studies to explore the roles of seasonal modification in the cuticle and in epidermal and hemolymph antifreeze proteins (AFPs). Seasonal cuticular modifications contribute to the inhibition of inoculative freezing since more cold-hardy larvae froze inoculatively when epicuticular waxes were removed with hexane and, in in vitro chamber experiments, cuticle patches (with the underlying epidermis removed) from winter larvae provided greater protection from inoculative freezing than did cuticle patches from summer larvae. The results indicate that seasonal modifications in epidermal and hemolymph AFPs contribute most strongly to the inhibition of inoculative freezing. Subcuticular epidermal AFPs were present in immunocytochemically labeled transverse sections of winter larvae but were absent in summer ones. Winter integument patches (cuticle with epidermis) were more resistant to inoculative freezing than were summer integument patches. Integument patches resisted inoculative freezing as well as live winter-collected larvae only when hemolymph AFP was added. The results also suggest that some integumentary ice nucleators are removed in cold-hardy larvae and that AFP promotes supercooling by inhibiting the activity of these nucleators.

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