Morphogenic competence of Vitis rupestris S. secondary somatic embryos with a long culture history

2001 ◽  
Vol 20 (4) ◽  
pp. 279-284 ◽  
Author(s):  
L. Martinelli ◽  
E. Candioli ◽  
D. Costa ◽  
V. Poletti ◽  
N. Rascio
Keyword(s):  
Somatic Embryos ◽  
Vitis Rupestris ◽  
Culture History ◽  
Secondary Somatic Embryos

scholarly journals The evaluation of java fine flavor cocoa propagation through somatic embryogenesis technique for germplasm preservation

2021 ◽  
Vol 306 ◽  
pp. 01056
Author(s):  
Sulistyani Pancaningtyas
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Basic Structure ◽  
Embryonic Cells ◽  
Regeneration Rate ◽  
Germplasm Preservation ◽  
Embryogenic Callus Formation ◽  
Secondary Somatic Embryos

Somatic embryogenesis is one of the newest technology that applied for the mass production of cocoa. This research aims to evaluate the regeneration rate of somatic embryos through somatic embryogenesis propagation techniques on java fine flavor cocoa. Cultivars in this study are ICCRI 01, ICCRI 02, DR 1, DR 2, DRC 16, DR 38, PNT 16, and PNT 30. Observations include parameters to determine the percentage of primary callus and embryogenic callus formation and the number of somatic embryos produced. Based on data, the ability of callus to produce primary embryos is highly dependent on plant cultivars and explant sources. Five cultivars showed a higher regeneration rate using explants from the petal part, while the rest showed a higher regeneration rate using explants from the staminode section. Embryogenic callus from each cacao cultivar has the same basic structure: a nodular friable structure consisting of many embryonic cells. Some fine flavor cacao cultivars that were able to produce callus and primary somatic embryos could not produce secondary somatic embryos and plantlets. However, two cultivars, which had low potential in producing primary embryos, had the high ability to produce secondary somatic embryos and develop into plantlets.

scholarly journals EFFECTS OF EXPLANT TYPE, CULTURE MEDIA AND PICLORAM AND DICAMBA GROWTH REGULATORS ON INDUCTION AND PROLIFERATION OF SOMATIC EMBRYOS IN EUCALYPTUS GRANDIS X E. UROPHYLLA1

2017 ◽  
Vol 41 (5) ◽  
Author(s):  
Luciana Coelho de Moura ◽  
Aloisio Xavier ◽  
Ana Claudia Ferreira da Cruz ◽  
Ricardo Gallo ◽  
Kellen Cristina Gatti ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Somatic Embryos ◽  
Plant Material ◽  
Culture Media ◽  
Eucalyptus Grandis ◽  
Induction Phase ◽  
Explant Type ◽  
Liquid Media ◽  
Embryogenic Calli ◽  
Secondary Somatic Embryos

ABSTRACT The objective of the present study was to test the effects of explant type, auxin concentrations, culture media, and auxin concentrations on the induction and proliferation of somatic embryos of Eucalyptus grandis x E. urophylla. Seeds and cotyledons were used as explants and inoculated in culture media containing 1.13, 2.26, 3.39 and 4.52 µM dicamba or 4.14, 10.35, 20.71 and 31.06 µM picloram. Embryogenic calli induced in the picloram treatments were used as explants and inoculated in semisolid or liquid media containing 4.14, 10.35, 20.71 and 31.06 µM picloram and keeping the origin of the embryogenic callus (seeds or cotyledons) and the concentration of picloram in those who were in the induction phase. Statistical, descriptive and anatomical analyses were performed. Induction of somatic pro-embryos into the juvenile plant material of Eucalyptus grandis x E. urophylla was performed using seeds or cotyledons as the source of explants, with the addition of dicamba and picloram as growth regulators. The use of cotyledons as a source of explants and the concentration of 4.1 µM picloram added to the culture media resulted in a higher induction of somatic pro-embryos. Proliferation of secondary somatic embryos was achieved using liquid medium added with picloram.

scholarly journals Morpho-anatomical characterization of secondary somatic embryogenesis in Azadirachta indica (Meliaceae)

2018 ◽  
pp. 7-20
Author(s):  
Maria Daniela Artigas Ramirez ◽  
Rafael Fernandez Da Silva
Keyword(s):  
Somatic Embryogenesis ◽  
Azadirachta Indica ◽  
Somatic Embryos ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Secondary Somatic Embryogenesis ◽  
Efficient System ◽  
Whole Process ◽  
Secondary Somatic Embryos

Background and Aims: Meliaceae species are extremely recalcitrant during germination and in vitro processes. Therefore, this research focuses on characterization and optimization of a highly efficient system by secondary somatic embryogenesis in Azadirachta indica, which is an important step for enhancing secondary metabolite production and regeneration in recalcitrant species.Material and Methods: Leaf and cotyledon sections were induced in MS medium supplemented with benzylaminopurine (BAP) alone, or combined with 1-naphthaleneacetic acid (NAA) and, abscisic acid (BA) with thidiazuron (TDZ).Key results: Azadirachta indica developed primary somatic embryos with BAP. Shoot and root formation occurred at low concentrations of BAP, while somatic embryogenesis was favored under high levels of BAP or TDZ. Primary and secondary somatic embryos were evidenced continuously and asynchronously. The highest amount of somatic embryos was obtained with cytokinins. However, the concentration might be significant to differentiate between primary and secondary embryos. Moreover, the auxins are key for inducing histodifferentiation in embryos. Shoot induction occurred after transfer of the embryos to hormone-free MS medium. The shoots were rooted in MS1/2.Conclusions: The secondary somatic embryos were distinguished and characterized during the whole process and the efficient system was established with cotyledon sections at short term, which offers several advantages such as the production of metabolites.

Acclimatization of secondary somatic embryos derived plants of Eucalyptus globulus Labill.: an ultrastructural approach

Trees ◽  
2010 ◽  
Vol 25 (3) ◽  
pp. 383-392 ◽  
Author(s):  
Glória Pinto ◽  
Sónia Silva ◽  
João Loureiro ◽  
Armando Costa ◽  
Maria Celeste Dias ◽  
...  
Keyword(s):  
Somatic Embryos ◽  
Eucalyptus Globulus ◽  
Secondary Somatic Embryos
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