Essential oils enhanced by ultra-high carbon dioxide levels from Lamiaceae species grown in vitro and in vivo

2001 ◽  
Vol 20 (4) ◽  
pp. 361-368 ◽  
Author(s):  
B. Tisserat ◽  
S.F. Vaughn
1969 ◽  
Vol 22 (4) ◽  
pp. 1061
Author(s):  
NG Nair ◽  
NH White ◽  
DM Griffin ◽  
Suzanne Blair§

The number of mitochondria apparently vary with the energy requirements of the cell (see Rouiller 1960). Although swelling and multiplication of mitochondria have been observed to occur in cells, a quantitative analysis of these changes has not been recorded. Matile and Bahr (1968) have recently provided electron micro-scopic evidence of the heterogeneity of density, mass, and volume of the mitochon-drial population in respiring baker's yeast. There are reports in the literature of the complete absence of mitochondria in yeast cells growing under anaerobic conditions (see Marchant and Smith 1968). Griffin and Nair (1968) demonstrated that the growth of Sclerotium rolfsii was inhibited by concentrations of oxygen below 4% and by concentrations of carbon dioxide above 0�03 %. It was, therefore, thought worthwhile to study the changes in mitochondria of this fungus when the cells are subjected to external stresses of low oxygen and high carbon dioxide levels.


2001 ◽  
Vol 79 (10) ◽  
pp. 1129-1133
Author(s):  
Marie-Claire Héloir ◽  
Jean-Claude Fournioux

This study reveals that morphogenesis of grapevine plants (Vitis vinifera L.) produced in vitro under carbon dioxide enriched atmosphereis largely determined by the morphogenetic pattern of vitroplants on which microcuttings were harvested. The presence of tendrils on shoots or shoot parts from which microcuttings were made was essential to obtain a high percentage of adult vitroplants with ternary sequence of tendrils. Therefore, under in vitro culture conditions, the ability of the axillary meristem to produce tendrils is closely correlated with the ability of the apical meristem from which it originated. The interest of these results is discussed from a fundamental standpoint. It allows to suggest an improved process of adult vitroplants production under high carbon dioxide level in which the relative importance of the selection of microcuttings and the number of subcultures is defined.Key words: maturation, micropropagation, tendrils, Vitis vinifera.


1997 ◽  
Vol 7 (3) ◽  
pp. 282-289 ◽  
Author(s):  
Brent Tisserat ◽  
Christopher Herman ◽  
Robert Silman ◽  
Rodney J. Bothast

A continuous CO2 flow system was used to study the growth of carrot (Daucus carota L.), citrus (Citrus macrophylla L.), kale (Brassica oleracea L.), lettuce (Lactuca sativa L.), radish (Raphanus sativus L.), and tomato (Lycopersicum esculentum L.) cultures in vitro under photoautotrophic, photomixotrophic, and heterotrophic conditions. Lettuce plantlets were grown on Murashige and Skoog medium with 0%, 0.3%, 1%, and 3% sucrose within flow chambers containing 350, 750, 1500, 3000, 10,000, 30,000, and 50,000 μL·L−1 CO2. Increasing the levels of CO2, especially at the ultra-high levels (i.e., ≥3,000 μL·L−1 CO2), increased fresh weight, shoot length, leaf number, leaf length, leaf width, root number, and root length for plantlets grown regardless of sucrose levels tested compared to plantlets grown at normal atmospheric CO2 levels, i.e., 350 μL·L-1. For example, fresh weights of lettuce plantlets grown on medium containing 0% or 3% sucrose increased 11- and 13-fold, respectively, when supplemented with 30,000 μL·L-1 CO2 compared to growth of lettuce plantlets grown on the same media without CO2 enrichment. Similar fold increases in growth responses were obtained with carrot, citrus, kale, radish, and tomato plantlets grown in atmospheres enriched with high CO2 levels, elevated from 3000 to 30,000 μL·L-1. Optimum CO2 concentration varied among species, suggesting a species-related response. Varying the rate of CO2 application between 250, 500, 1500, or 2000 mL·min-1 did not effect the rate of growth of lettuce plantlets. The passive diffusion continuous flow-through system presented in this paper is inexpensive, easily constructed, and allows for testing ultra-high CO2 levels on plant culture growth in vitro.


HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 539B-539
Author(s):  
Mary E. Mangrich ◽  
Mikal E. Saltveit

Crops with origins in tropics and subtropics undergo physiological injury when subjected to nonfreezing temperatures below 12°C. Application of heat and chemical shocks to tissue prior to chilling induces chilling tolerance. This study was undertaken to investigate the effects of low oxygen and high carbon dioxide atmospheres on subsequent chilling tolerance. Cucumber seedlings (Cucumis sativus L., cv. Poinsett 76) with radicles 8 to 12 mm long were subjected to 0% to 21% oxygen and/or 0% to 20% CO2 atmospheres for 0 to 72 hr at 2.5 or 15°C. After chilling, they were placed at 25°C for three additional days. Radicle growth was used to assess chilling injury. Modification of the individual germination plates was necessary to ensure seedling exposure to the desired atmospheres. Chilling injury was reduced by exposure to oxygen levels below 1% and to carbon dioxide levels above 5%. Effects of brief exposures were small in comparison to prolonged exposures during chilling. Seed to seed variability was high and obscured some results. The effects of the various atmospheres were greater with the less vigorous seedlings.


1969 ◽  
Vol 28 (4) ◽  
pp. 554-556 ◽  
Author(s):  
P. H. Knowlton ◽  
W. H. Hoover ◽  
B. R. Poulton

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