Heterologous Expression of the Phycomyces blakesleeanus Phytoene Dehydrogenase Gene ( carB ) in Mucor circinelloides

1999 ◽  
Vol 39 (5) ◽  
pp. 259-264 ◽  
Author(s):  
María José Ruiz-Hidalgo
Keyword(s):  
Heterologous Expression ◽  
Mucor Circinelloides ◽  
Phycomyces Blakesleeanus ◽  
Dehydrogenase Gene ◽  
Phytoene Dehydrogenase

Inhibition of Carotene Biosynthesis in Cell Extracts of Phycomyces blakesleeanus

1984 ◽  
Vol 39 (5) ◽  
pp. 460-463 ◽  
Author(s):  
Peter M. Bramley ◽  
Ian E. Clarke ◽  
Gerhard Sandmann ◽  
Peter Böger
Keyword(s):  
Competitive Inhibition ◽  
Enzymic Activity ◽  
Phycomyces Blakesleeanus ◽  
Cell Extracts ◽  
Phytoene Dehydrogenase ◽  
Β Carotene

Cell extracts of the C115 (β-carotene-accumulating) strain of Phycomyces blakesleeanus were incubated with either [2-14C]MVA or [1-14C]IPP and a range of possible inhibitors of carotenogenesis, including bleaching herbicides, biphenyl compounds and geranylacetone. Several of these compounds were potent inhibitors of β-carotene formation and caused the accumulation of phytoene. No other carotenes were found to accumulate, in vitro.The structures of these inhibitors, compared to that of phytoene, suggest that they affect the enzymic activity of “phytoene dehydrogenase”, possibly by competitive inhibition.

Cloning and characterization of a phytoene dehydrogenase gene from marine yeast Rhodosporidium diobovatum

2015 ◽  
Vol 107 (4) ◽  
pp. 1017-1027 ◽  
Author(s):  
Wenjing Guo ◽  
Yuxuan Liu ◽  
Xin Yan ◽  
Mingyi Liu ◽  
Hui Tang ◽  
...  
Keyword(s):  
Marine Yeast ◽  
Dehydrogenase Gene ◽  
Phytoene Dehydrogenase ◽  
Rhodosporidium Diobovatum

scholarly journals Blakeslea trispora Genes for Carotene Biosynthesis

2004 ◽  
Vol 70 (9) ◽  
pp. 5589-5594 ◽  
Author(s):  
M. Rodríguez-Sáiz ◽  
B. Paz ◽  
J. L. de la Fuente ◽  
M. J. López-Nieto ◽  
W. Cabri ◽  
...  
Keyword(s):  
Stop Codon ◽  
Mucor Circinelloides ◽  
Blakeslea Trispora ◽  
Wild Type ◽  
Orthologous Genes ◽  
Carotenoid Production ◽  
Phytoene Dehydrogenase ◽  
Lycopene Cyclase ◽  
Type Strains ◽  
Β Carotene

ABSTRACT We cloned the carB and carRA genes involved in β-carotene biosynthesis from overproducing and wild-type strains of Blakeslea trispora. The carB gene has a length of 1,955 bp, including two introns of 141 and 68 bp, and encodes a protein of 66.4 kDa with phytoene dehydrogenase activity. The carRA gene contains 1,894 bp, with a single intron of 70 bp, and encodes a protein of 69.6 kDa with separate domains for lycopene cyclase and phytoene synthase. The estimated transcript sizes for carB and carRA were 1.8 and 1.9 kb, respectively. CarB from the β-carotene-overproducing strain B. trispora F-744 had an S528R mutation and a TAG instead of a TAA stop codon. The overproducing strain also had a P143S mutation in CarRA. Both B. trispora genes could complement mutations in orthologous genes in Mucor circinelloides and could be used to construct transformed strains of M. circinelloides that produced higher levels of β-carotene than did the nontransformed parent. The results show that these genes are conserved across the zygomycetes and that the B. trispora carB and carRA genes are functional and potentially useable to increase carotenoid production.

Molecular characterization and heterologous expression of quinate dehydrogenase gene from Gluconobacter oxydans IFO3244

2010 ◽  
Vol 75 (4) ◽  
pp. 452-459 ◽  
Author(s):  
A. S. Vangnai ◽  
W. Promden ◽  
W. De-Eknamkul ◽  
K. Matsushita ◽  
H. Toyama
Keyword(s):  
Heterologous Expression ◽  
Molecular Characterization ◽  
Gluconobacter Oxydans ◽  
Dehydrogenase Gene

Light regulates a Phycomyces blakesleeanus gene family similar to the carotenogenic repressor gene of Mucor circinelloides

2020 ◽  
Vol 124 (5) ◽  
pp. 338-351 ◽  
Author(s):  
Víctor G. Tagua ◽  
Eusebio Navarro ◽  
Gabriel Gutiérrez ◽  
Victoriano Garre ◽  
Luis M. Corrochano
Keyword(s):  
Gene Family ◽  
Mucor Circinelloides ◽  
Phycomyces Blakesleeanus ◽  
Repressor Gene

The phytoene dehydrogenase gene of Phycomyces : regulation of its expression by blue light and vitamin A

1997 ◽  
Vol 253 (6) ◽  
pp. 734-744 ◽  
Author(s):  
M. J. Ruiz-Hidalgo ◽  
E. P. Benito ◽  
G. Sandmann ◽  
A. P. Eslava
Keyword(s):  
Vitamin A ◽  
Blue Light ◽  
Dehydrogenase Gene ◽  
Phytoene Dehydrogenase

Heterologous expression of astaxanthin biosynthesis genes in Mucor circinelloides

2005 ◽  
Vol 69 (5) ◽  
pp. 526-531 ◽  
Author(s):  
Tamás Papp ◽  
Antonio Velayos ◽  
Tibor Bartók ◽  
Arturo P. Eslava ◽  
Csaba Vágvölgyi ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Mucor Circinelloides
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