scholarly journals Mechanism and pharmacological specificity of dUTPase-mediated protection from DNA damage and cytotoxicity in human tumor cells

1998 ◽  
Vol 42 (5) ◽  
pp. 357-362 ◽  
Author(s):  
Leslie A. Parsels ◽  
Joshua D. Parsels ◽  
Lois M. Wagner ◽  
Tania L. Loney ◽  
Eric H. Radany ◽  
...  
Keyword(s):  
Dna Damage ◽  
Tumor Cells ◽  
Human Tumor ◽  
Human Tumor Cells

scholarly journals Induction of Oxidative DNA Damage in Bovine Herpesvirus 1 Infected Bovine Kidney Cells (MDBK Cells) and Human Tumor Cells (A549 Cells and U2OS Cells)

Viruses ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 393 ◽  
Author(s):  
Liqian Zhu ◽  
Xiaotian Fu ◽  
Chen Yuan ◽  
Xinyi Jiang ◽  
Gaiping Zhang
Keyword(s):  
Dna Damage ◽  
Virus Infection ◽  
Tumor Cells ◽  
Oxidative Dna Damage ◽  
A549 Cells ◽  
Human Tumor ◽  
Human Tumor Cells ◽  
Bovine Herpesvirus 1 ◽  
Mdbk Cells ◽  
Herpesvirus 1

Bovine herpesvirus 1 (BoHV-1) is an important pathogen of cattle that causes lesions in mucosal surfaces, genital tracts and nervous systems. As a novel oncolytic virus, BoHV-1 infects and kills numerous human tumor cells. However, the mechanisms underlying the virus-induced cell damages are not fully understood. In this study, we demonstrated that virus infection of MDBK cells induced high levels of DNA damage, because the percentage of comet tail DNA (tailDNA%) determined by comet assay, a direct indicator of DNA damage, and the levels of 8-hydroxyguanine (8-oxoG) production, an oxidative DNA damage marker, consistently increased following the virus infection. The expression of 8-oxoguanine DNA glycosylase (OGG-1), an enzyme responsible for the excision of 8-oxoG, was significantly decreased due to the virus infection, which corroborated with the finding that BoHV-1 infection stimulated 8-oxoG production. Furthermore, the virus replication in human tumor cells such as in A549 cells and U2OS cells also induced DNA damage. Chemical inhibition of reactive oxidative species (ROS) production by either ROS scavenger N-Acetyl-l-cysteine or NOX inhibitor diphenylene iodonium (DPI) significantly decreased the levels of tailDNA%, suggesting the involvement of ROS in the virus induced DNA lesions. Collectively, these results indicated that BoHV-1 infection of these cells elicits oxidative DNA damages, providing a perspective in understanding the mechanisms by which the virus induces cell death in both native host cells and human tumor cells.

Dose-Rate Effect for DNA Damage Induced by Ionizing Radiation in Human Tumor Cells

1994 ◽  
Vol 138 (1) ◽  
pp. S93 ◽  
Author(s):  
J. M. Ruiz de Almodóvar ◽  
C. Bush ◽  
J. H. Peacock ◽  
G. G. Steel ◽  
S. J. Whitaker ◽  
...  
Keyword(s):  
Dna Damage ◽  
Ionizing Radiation ◽  
Dose Rate ◽  
Tumor Cells ◽  
Human Tumor ◽  
Rate Effect ◽  
Human Tumor Cells ◽  
Dose Rate Effect

scholarly journals The canonical NF-κB pathway differentially protects normal and human tumor cells from ROS-induced DNA damage

2012 ◽  
Vol 24 (11) ◽  
pp. 2007-2023 ◽  
Author(s):  
Alexandros Sfikas ◽  
Christina Batsi ◽  
Evangelia Tselikou ◽  
George Vartholomatos ◽  
Nikolaos Monokrousos ◽  
...  
Keyword(s):  
Dna Damage ◽  
Tumor Cells ◽  
Human Tumor ◽  
Human Tumor Cells

scholarly journals A Novel Role for Pyruvate Kinase M2 as a Corepressor for P53 during the DNA Damage Response in Human Tumor Cells

2016 ◽  
Vol 291 (50) ◽  
pp. 26138-26150 ◽  
Author(s):  
Li Xia ◽  
Xin-Ran Wang ◽  
Xiao-Ling Wang ◽  
Su-Hui Liu ◽  
Xiao-Wei Ding ◽  
...  
Keyword(s):  
Dna Damage ◽  
Tumor Cells ◽  
Pyruvate Kinase ◽  
Dna Damage Response ◽  
Human Tumor ◽  
Human Tumor Cells ◽  
Pyruvate Kinase M2 ◽  
Damage Response
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