Imidazoacridinones arrest cell-cycle progression in the G2 phase of L1210 cells

1996 ◽  
Vol 38 (1) ◽  
pp. 39-44 ◽  
Author(s):  
E. Augustin ◽  
Denys N Wheatley ◽  
Justin Lamb ◽  
J. Konopa
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
L1210 Cells ◽  
G2 Phase ◽  
Arrest Cell Cycle Progression

scholarly journals Cubic Membranes Formation in Synchronized Human Hepatocellular Carcinoma Cells Reveals a Possible Role as a Structural Antioxidant Defense System in Cell Cycle Progression

2020 ◽  
Vol 8 ◽  
Author(s):  
Deqin Kong ◽  
Rui Liu ◽  
Jiangzheng Liu ◽  
Qingbiao Zhou ◽  
Jiaxin Zhang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Cycle ◽  
Cell Cycle Progression ◽  
S Phase ◽  
Carcinoma Cells ◽  
Human Hepatocellular Carcinoma ◽  
Cycle Progression ◽  
Hepatocellular Carcinoma Cells ◽  
G2 Phase ◽  
Human Hepatocellular Carcinoma Cells

Cubic membranes (CMs) represent unique biological membrane structures with highly curved three-dimensional periodic minimal surfaces, which have been observed in a wide range of cell types and organelles under various stress conditions (e. g., starvation, virus-infection, and oxidation). However, there are few reports on the biological roles of CMs, especially their roles in cell cycle. Hence, we established a stable cell population of human hepatocellular carcinoma cells (HepG2) of 100% S phase by thymidine treatment, and determined certain parameters in G2 phase released from S phase. Then we found a close relationship between CMs formation and cell cycle, and an increase in reactive oxygen species (ROS) and mitochondrial function. After the synchronization of HepG2 cells were induced, CMs were observed through transmission electron microscope in G2 phase but not in G1, S and M phase. Moreover, the increased ATP production, mitochondrial and intracellular ROS levels were also present in G2 phase, which demonstrated a positive correlation with CMs formation by Pearson correlation analysis. This study suggests that CMs may act as an antioxidant structure in response to mitochondria-derived ROS during G2 phase and thus participate in cell cycle progression.

scholarly journals DNA Damage during G2 Phase Does Not Affect Cell Cycle Progression of the Green Alga Scenedesmus quadricauda

PLoS ONE ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. e19626 ◽  
Author(s):  
Monika Hlavová ◽  
Mária Čížková ◽  
Milada Vítová ◽  
Kateřina Bišová ◽  
Vilém Zachleder
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Green Alga ◽  
Cell Cycle Progression ◽  
Scenedesmus Quadricauda ◽  
Cycle Progression ◽  
Affect Cell Cycle Progression ◽  
G2 Phase ◽  
Alga Scenedesmus Quadricauda

scholarly journals HIV-1 Vif promotes the G1- to S-phase cell-cycle transition

Blood ◽  
2011 ◽  
Vol 117 (4) ◽  
pp. 1260-1269 ◽  
Author(s):  
Jiangfang Wang ◽  
Emma L. Reuschel ◽  
Jason M. Shackelford ◽  
Lauren Jeang ◽  
Debra K. Shivers ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
S Phase ◽  
Phase Cell ◽  
Small Interfering Rnas ◽  
Cycle Progression ◽  
Viral Infectivity Factor ◽  
G2 Phase ◽  
Regulate Cell Cycle Progression ◽  
Hiv 1

AbstractHIV-1 depends on host-cell resources for replication, access to which may be limited to a particular phase of the cell cycle. The HIV-encoded proteins Vpr (viral protein R) and Vif (viral infectivity factor) arrest cells in the G2 phase; however, alteration of other cell-cycle phases has not been reported. We show that Vif drives cells out of G1 and into the S phase. The effect of Vif on the G1-to-S transition is distinct from its effect on G2, because G2 arrest is Cullin5-dependent, whereas the G1-to-S progression is Cullin5-independent. Using mass spectrometry, we identified 2 novel cellular partners of Vif, Brd4 and Cdk9, both of which are known to regulate cell-cycle progression. We confirmed the interaction of Vif and Cdk9 by immunoprecipitation and Western blot, and showed that small interfering RNAs (siRNAs) specific for Cdk9 inhibit the Vif-mediated G1-to-S transition. These data suggest that Vif regulates early cell-cycle progression, with implications for infection and latency.

scholarly journals Human Cytomegalovirus UL69 Protein Induces Cells To Accumulate in G1 Phase of the Cell Cycle

1999 ◽  
Vol 73 (1) ◽  
pp. 676-683 ◽  
Author(s):  
Mansuo Lu ◽  
Thomas Shenk
Keyword(s):  
Cell Cycle ◽  
Human Cytomegalovirus ◽  
Cell Cycle Progression ◽  
Human Fibroblasts ◽  
Protein A ◽  
S Phase ◽  
Cycle Progression ◽  
Multiple Points ◽  
Cell Cycle Block ◽  
Arrest Cell Cycle Progression

ABSTRACT Earlier studies have revealed that human cytomegalovirus rapidly inhibits the growth of fibroblasts, blocking cell cycle progression at multiple points, including the G1-to-S-phase transition. The present study demonstrates that the UL69 protein, a virus-encoded constituent of the virion, is able to arrest cell cycle progression when introduced into uninfected cells. Expression of the UL69 protein causes U2 OS cells and primary human fibroblasts to accumulate within the G1 compartment of the cell cycle, and serum fails to induce the progression of quiescent human fibroblasts into the S phase when the protein is present. Therefore, the UL69 protein is at least partially responsible for the cell cycle block that is instituted after infection of permissive cells with human cytomegalovirus.

The ERK-RSK1 activation by growth factors at G2 phase delays cell cycle progression and reduces mitotic aberrations

2008 ◽  
Vol 20 (7) ◽  
pp. 1349-1358 ◽  
Author(s):  
Hyun-Ja Nam ◽  
Sujeong Kim ◽  
Min-Woo Lee ◽  
Bok-Soon Lee ◽  
Toshihiro Hara ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Growth Factors ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
G2 Phase

Effect of n-butyrate on cell cycle progression and in situ chromatin structure of L1210 cells

1981 ◽  
Vol 136 (2) ◽  
pp. 279-293 ◽  
Author(s):  
Zbigniew Darzynkiewicz ◽  
Frank Traganos ◽  
Shao-Bai Xue ◽  
Myron R. Melamed
Keyword(s):  
Cell Cycle ◽  
Chromatin Structure ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
L1210 Cells

scholarly journals Transient expression of RAD51 in the late G2-phase is required for cell cycle progression in synchronousPhysarumcells

2014 ◽  
Vol 19 (10) ◽  
pp. 755-765 ◽  
Author(s):  
Anthony Le Cigne ◽  
Vanessa Menil-Philippot ◽  
Fabrice Fleury ◽  
Masayuki Takahashi ◽  
Christophe Thiriet
Keyword(s):  
Cell Cycle ◽  
Transient Expression ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
G2 Phase
Sign in / Sign up

Export Citation Format

Share Document