Conversion of mitomycin C to 2,7-diaminomitosene and 10-decarbamoyl 2,7-diaminomitosene in tumour tissue in vivo

1995 ◽  
Vol 35 (4) ◽  
pp. 318-322
Author(s):  
Linda Chirrey ◽  
J. Cummings ◽  
Gavin W. Halbert ◽  
John F. Smyth
Keyword(s):  
Mitomycin C ◽  
Tumour Tissue

Conversion of mitomycin C to 2,7-diaminomitosene and 10-decarbamoyl 2,7-diaminomitosene in tumour tissue in vivo

1995 ◽  
Vol 35 (4) ◽  
pp. 318-322 ◽  
Author(s):  
Linda Chirrey ◽  
Jeffrey Cummings ◽  
Gavin W. Halbert ◽  
John F. Smyth
Keyword(s):  
Mitomycin C ◽  
Tumour Tissue

Diagnostic imaging of the larynx: autofluorescence of laryngeal tumours using the helium-cadmium laser

1995 ◽  
Vol 109 (2) ◽  
pp. 108-110 ◽  
Author(s):  
Meredydd Lloyd Harries ◽  
Stephen Lam ◽  
Calum MacAulay ◽  
Jianan Qu ◽  
Branko Palcic
Keyword(s):  
Pilot Study ◽  
Diagnostic Imaging ◽  
Vocal Folds ◽  
Tumour Tissue ◽  
Laryngeal Tumours ◽  
Bronchial Tissue ◽  
Fluorescence Images ◽  
Detection And Localization

AbstractThe use of tissue autofluorescence for the detection and localization of cancer of the larynx is described. In this pilot study, eight patients with probable carcinoma of the vocal folds underwent laryngoscopy in which the tissue autofluorescence spectra of normal and pathologically confirmed tumour tissue were acquiredin vivo. Fluorescence images of the suspect areas were also acquired using the LIFE system (Xillix Technologies Corp.). The results suggest that the autofluorescence properties of laryngeal tissue, under 442 nm illumination, are similar to those of bronchial tissue and that the LIFE system has the potential to increase the accuracy of staging of cancer of the larynx and also to allow earlier diagnosis of tumours and theirrecurrence

β-carotene as an inhibitor of benzo(a)pyrene and mitomycin C induced chromosomal breaks in the bone marrow of mice

1985 ◽  
Vol 27 (5) ◽  
pp. 598-602 ◽  
Author(s):  
A. S. Raj ◽  
Morris Katz
Keyword(s):  
Bone Marrow ◽  
Mitomycin C ◽  
Micronucleus Assay ◽  
Leafy Vegetables ◽  
Hybrid Strain ◽  
Green Leafy Vegetables ◽  
Polychromatic Erythrocytes ◽  
Chromosomal Breaks ◽  
Β Carotene

Female mice of hybrid strain B6C3F1, 8–10 weeks old, were fed on powdered food with or without β-carotene (100 mg/kg food). After 1 week of these diets, some of each group of mice were injected i.p. with either benzo(a)pyrene (150 mg/kg) in dimethyl sulfoxide, or mitomycin C (1 mg/kg) in distilled water. In the course of separate experiments, bone marrow samples were collected at various intervals after injection for analysis in the in vivo bone marrow micronucleus assay. At the time at which the maximum induction was observed, which coincided between experiments, the frequency of micronuclei induced by benzo(a)pyrene was reduced by 41–61% and that induced by mitomycin C was reduced by 44–71% in the presence of β-carotene. β-carotene is widely distributed in plant material such as carrots and green leafy vegetables and, as such, is a component of the human diet. Our results suggest that β-carotene provides significant protection against the genotoxicity of benzo(a)pyrene and mitomycin C.Key words: β-carotene, inhibitor, chromosomal breaks, micronucleated polychromatic erythrocytes.

Phenotypic correction of Fanconi anemia group C knockout mice

Blood ◽  
2000 ◽  
Vol 95 (2) ◽  
pp. 700-704 ◽  
Author(s):  
Kimberly A. Gush ◽  
Kai-Ling Fu ◽  
Markus Grompe ◽  
Christopher E. Walsh
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Mitomycin C ◽  
Fanconi Anemia ◽  
Bone Marrow Cells ◽  
Bone Marrow Failure ◽  
Genetic Disorder ◽  
Hematopoietic Stem ◽  
Marrow Cells

Fanconi anemia (FA) is a genetic disorder characterized by bone marrow failure, congenital anomalies, and a predisposition to malignancy. FA cells demonstrate hypersensitivity to DNA cross-linking agents, such as mitomycin C (MMC). Mice with a targeted disruption of the FANCC gene (fancc −/− nullizygous mice) exhibit many of the characteristic features of FA and provide a valuable tool for testing novel therapeutic strategies. We have exploited the inherent hypersensitivity offancc −/− hematopoietic cells to assay for phenotypic correction following transfer of the FANCC complementary DNA (cDNA) into bone marrow cells. Murine fancc −/− bone marrow cells were transduced with the use of retrovirus carrying the humanfancc cDNA and injected into lethally irradiated recipients. Mitomycin C (MMC) dosing, known to induce pancytopenia, was used to challenge the transplanted animals. Phenotypic correction was determined by assessment of peripheral blood counts. Mice that received cells transduced with virus carrying the wild-type gene maintained normal blood counts following MMC administration. All nullizygous control animals receiving MMC exhibited pancytopenia shortly before death. Clonogenic assay and polymerase chain reaction analysis confirmed gene transfer of progenitor cells. These results indicate that selective pressure promotes in vivo enrichment offancc-transduced hematopoietic stem/progenitor cells. In addition, MMC resistance coupled with detection of the transgene in secondary recipients suggests transduction and phenotypic correction of long-term repopulating stem cells.

scholarly journals In vivo Role of NAD(P)H:Quinone Oxidoreductase 1 in Metabolic Activation of Mitomycin C and Bone Marrow Cytotoxicity

2007 ◽  
Vol 67 (17) ◽  
pp. 7966-7971 ◽  
Author(s):  
Anbu Karani Adikesavan ◽  
Roberto Barrios ◽  
Anil K. Jaiswal
Keyword(s):  
Bone Marrow ◽  
Mitomycin C ◽  
Metabolic Activation

STEROID BIOSYNTHESIS IN VITRO BY A VIRILIZING SUPRARENAL TUMOUR

1963 ◽  
Vol 44 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Roversi G. D. ◽  
Polvani F. ◽  
Bompiani A. ◽  
Neher R.
Keyword(s):  
Adrenal Adenoma ◽  
Tumour Tissue ◽  
Side Chain ◽  
Steroid Biosynthesis ◽  
Before And After

ABSTRACT A case of virilizing adrenal adenoma is described. The tumour was incubated with progesterone-4-14C. In the extract the following steroids were identified chromatographically, in order of decreasing quantity and radioactivity: 17α-hydroxyprogesterone, androst-4-ene-3,17-dione, corticosterone (11β,21 -dihydroxy-pregn-4-ene-3,20-dione), cortisol (11β,17,21-trihydroxy-pregn-4-ene-3,20-dione) and 11-deoxycortisol. This indicates either an increase in 17α-hydroxylation and side chain split, or a partial blockage of 21-hydroxylation, or a combination of both in the tumour tissue. The absence of the 3β-hydroxy-dehydrogenase demonstrated histochemically in the tumour and the examination of the urinary 17-ketosteroids before and after removal of the neoplasm, suggested the same abnormal biosynthetic pattern in vivo with regard to the level of the endogenous Δ5-precursors.

Autoradiographic study of chick limb cartilage in vivo and in vitro and its response to mitomycin C (MMC)

1975 ◽  
Vol 93 (3) ◽  
pp. 440-446 ◽  
Author(s):  
Shamer Singh ◽  
D.N. Sinha ◽  
G.C. Prasad
Keyword(s):  
Mitomycin C ◽  
Autoradiographic Study
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