Preparation of an organic solvent-tolerant strain from baker's yeast

2001 ◽  
Vol 55 (4) ◽  
pp. 476-479 ◽  
Author(s):  
T. Kawamoto ◽  
T. Kanda ◽  
A. Tanaka
Keyword(s):  
Organic Solvent ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Tolerant Strain ◽  
Organic Solvent Tolerant ◽  
Solvent Tolerant

Organic solvent stable protease isolation and characterization from organic solvent tolerant strain of Lysinibacillus sphaericus PAP02

Biologia ◽  
2016 ◽  
Vol 71 (9) ◽  
Author(s):  
Periasamy Anbu ◽  
Jae-Seong So ◽  
Byung-Ki Hur ◽  
Hyun-Shik Yun
Keyword(s):  
Organic Solvent ◽  
Lysinibacillus Sphaericus ◽  
Tolerant Strain ◽  
Isolation And Characterization ◽  
Organic Solvent Tolerant ◽  
Solvent Tolerant ◽  
Solvent Stable

AbstractIn this study, a bacterium producing solvent-stable protease was isolated from salt-enriched soil after incubation in benzene and toluene enriched-media and identified as

Efficient glycosylation of puerarin by an organic solvent-tolerant strain of Lysinibacillus fusiformis

2014 ◽  
Vol 57 ◽  
pp. 42-47 ◽  
Author(s):  
Siyuan Wang ◽  
Guiyou Liu ◽  
Wen Zhang ◽  
Nan Cai ◽  
Cheng Cheng ◽  
...  
Keyword(s):  
Organic Solvent ◽  
Tolerant Strain ◽  
Lysinibacillus Fusiformis ◽  
Organic Solvent Tolerant ◽  
Solvent Tolerant

scholarly journals Biochemical and Structural Characterization of Cross-Linked Enzyme Aggregates (CLEAs) of Organic Solvent Tolerant Protease

Catalysts ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 55 ◽  
Author(s):  
Muhammad Syafiq Mohd Razib ◽  
Raja Noor Zaliha Raja Abd Rahman ◽  
Fairolniza Mohd Shariff ◽  
Mohd Shukuri Mohamad Ali
Keyword(s):  
Structural Analysis ◽  
Organic Solvent ◽  
Average Diameter ◽  
Relative Activity ◽  
Biochemical Properties ◽  
Biochemical Interaction ◽  
Organic Solvent Tolerant ◽  
Random Aggregate ◽  
Solvent Tolerant ◽  
The Stability

Cross-linked enzyme aggregates (CLEAs) is an immobilization technique that can be used to customize enzymes under an optimized condition. Structural analysis on any enzyme treated with a CLEA remains elusive and has been less explored. In the present work, a method for preparing an organic solvent tolerant protease using a CLEA is disclosed and optimized for better biochemical properties, followed by an analysis of the structure of this CLEA-treated protease. The said organic solvent tolerant protease is a metalloprotease known as elastase strain K in which activity of the metalloprotease is measured by a biochemical interaction with azocasein. Results showed that when a glutaraldehyde of 0.02% (v/v) was used under a 2 h treatment, the amount of recovered activity in CLEA-elastase was highest. The recovered activity of CLEA-elastase and CLEA-elastase-SB (which was a CLEA co-aggregated with starch and bovine serum albumin (BSA)) were at an approximate 60% and 80%, respectively. The CLEA immobilization of elastase strain K allowed the stability of the enzyme to be enhanced at high temperature and at a broader pH. Both CLEA-elastase and CLEA-elastase-SB end-products were able to maintain up to 67% enzyme activity at 60 °C and exhibiting an enhanced stability within pH 5–9 with up to 90% recovering activity. By implementing a CLEA on the organic solvent tolerant protease, the characteristics of the organic solvent tolerant were preserved and enhanced with the presence of 25% (v/v) acetonitrile, ethanol, and benzene at 165%, 173%, and 153% relative activity. Structural analysis through SEM and dynamic light scattering (DLS) showed that CLEA-elastase had a random aggregate morphology with an average diameter of 1497 nm.

Thermostable and organic solvent-tolerant acid pectinase from Aspergillus terreus FP6: purification, characterization and evaluation of its phytopigment extraction potential

3 Biotech ◽  
2021 ◽  
Vol 11 (11) ◽  
Author(s):  
Rajrupa Bhattacharyya ◽  
Dibbyangana Mukhopadhyay ◽  
V. K. Nagarakshita ◽  
Sourav Bhattacharya ◽  
Arijit Das
Keyword(s):  
Organic Solvent ◽  
Aspergillus Terreus ◽  
Organic Solvent Tolerant ◽  
Solvent Tolerant
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