Development of a rapid and sensitive bioassay device using human cells immobilized in macroporous microcarriers for the on-site evaluation of environmental waters

2000 ◽  
Vol 54 (3) ◽  
pp. 432-438 ◽  
Author(s):  
R. Shoji ◽  
Y. Sakai ◽  
A. Sakoda ◽  
M. Suzuki
Keyword(s):  
Human Cells ◽  
Environmental Waters ◽  
Site Evaluation ◽  
Macroporous Microcarriers

Preservation of human cell bioassay device for the on-site evaluation of environmental waters

2002 ◽  
Vol 46 (11-12) ◽  
pp. 355-362 ◽  
Author(s):  
R. Shoji ◽  
Y. Sakai ◽  
A. Sakoda ◽  
M. Suzuki
Keyword(s):  
River Water ◽  
Low Density Lipoprotein ◽  
Water Quality Management ◽  
Density Lipoprotein ◽  
Hep G2 ◽  
Environmental Waters ◽  
Water Toxicity ◽  
Promising Tool ◽  
Site Evaluation ◽  
Daily Water

We have already developed a novel disposable bioassay device based on the low-density lipoprotein (LDL) uptaking activity of human hepatoblastoma Hep G2 cells in our previous work. However, this device is not readily applicable to evaluate river water toxicity on-site because it cannot be preserved for more than one week. In this work, we developed the method for preservation of the device to enable it to be preserved for at least one month. The device can be supplied to individual environmental sites without any facilities for cell culture. We can evaluate river water toxicity by 2 hours of exposure after thawing. Therefore, this kind of device could be a promising tool for daily water quality management.

Alpha-Tocopherol Protects Cultured Human Cells from the Acute Lethal Cytotoxicity of Dioxin

2002 ◽  
Vol 72 (3) ◽  
pp. 147-153 ◽  
Author(s):  
Kei-Ichi Hirai ◽  
Jie-Hong Pan ◽  
Ying-Bo Shui ◽  
Eriko Simamura ◽  
Hiroki Shimada ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Damage ◽  
Smooth Endoplasmic Reticulum ◽  
Dehydroascorbic Acid ◽  
Human Cells ◽  
Alpha Tocopherol ◽  
Cervical Cancer Cells ◽  
Cultured Human Cells ◽  
Nuclear Damage ◽  
Conjunctival Epithelial Cells

The possible protection of cultured human cells from acute dioxin injury by antioxidants was investigated. The most potent dioxin, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), caused vacuolization of the smooth endoplasmic reticulum and Golgi apparatus in cultured human conjunctival epithelial cells and cervical cancer cells. Subsequent nuclear damage included a deep irregular indentation resulting in cell death. A dosage of 30–40 ng/mL TCDD induced maximal intracellular production of H2O2 at 30 minutes and led to severe cell death (0–31% survival) at two hours. A dose of 1.7 mM alpha-tocopherol or 1 mM L-dehydroascorbic acid significantly protected human cells against acute TCDD injuries (78–97% survivals), but vitamin C did not provide this protection. These results indicate that accidental exposure to fatal doses of TCDD causes cytoplasmic free radical production within the smooth endoplasmic reticular systems, resulting in severe cytotoxicity, and that vitamin E and dehydroascorbic acid can protect against TCDD-induced cell damage.

Chromium oxidation state mapping in human cells

2003 ◽  
Vol 104 ◽  
pp. 289-292 ◽  
Author(s):  
R. Ortega ◽  
B. Fayard ◽  
M. Salomé ◽  
G. Devès ◽  
J. Susini
Keyword(s):  
Oxidation State ◽  
Human Cells ◽  
Chromium Oxidation

Transport of clozapine and its major metabolites into human cells of glial and neuronal origin

2004 ◽  
Vol 36 (05) ◽  
Author(s):  
U Henning ◽  
K Krieger ◽  
S Loeffler ◽  
A Klimke
Keyword(s):  
Human Cells

Lineage conversion of human cells to an adrenocortical phenotype: a new technology to study the adrenal gland

2015 ◽  
Author(s):  
Gerard Ruiz Babot ◽  
Irene Hadjidemetriou ◽  
Sharon Jane Ajodha ◽  
David Taylor ◽  
Norman Taylor ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
New Technology ◽  
Human Cells
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