Protein binding to supported lecithin bilayers controlled by the lipid phase state: a new concept for highly selective protein purification

1996 ◽  
Vol 25 (2) ◽  
pp. 151-153 ◽  
Author(s):  
A. Loidl-Stahlhofen ◽  
A. S. Ulrich ◽  
S. Kaufmann ◽  
T. M. Bayerl
Keyword(s):  
Protein Binding ◽  
Protein Purification ◽  
Phase State ◽  
Lipid Phase

Raman study of lipid phase state in freezing mammalian embryos and oocytes

Cryobiology ◽  
2018 ◽  
Vol 85 ◽  
pp. 165
Author(s):  
Konstantin A. Okotrub ◽  
Valentina I. Mokrousova ◽  
Sergei Ya. Amstislavsky ◽  
Nikolay V. Surovtsev
Keyword(s):  
Phase State ◽  
Lipid Phase ◽  
Mammalian Embryos ◽  
Raman Study

scholarly journals Presence and persistence of a highly ordered lipid phase state in the avian stratum corneum

2018 ◽  
Vol 221 (11) ◽  
pp. jeb176438 ◽  
Author(s):  
Alex M. Champagne ◽  
Victoria A. Pigg ◽  
Heather C. Allen ◽  
Joseph B. Williams
Keyword(s):  
Stratum Corneum ◽  
Phase State ◽  
Lipid Phase

scholarly journals Lipid Influence on the Structure of the Light Harvesting B 800—850 Proteins

1987 ◽  
Vol 42 (1-2) ◽  
pp. 109-117
Author(s):  
Joachim Peschke ◽  
Helmuth Möhwald
Keyword(s):  
Phase State ◽  
Transient Absorption ◽  
Photosynthetic Bacterium ◽  
Head Group ◽  
Lipid Phase ◽  
Elastic Model ◽  
Structural Protein ◽  
Protein Change ◽  
Lipid Environment ◽  
Lipid Head Group

Abstract Interaction of the antenna protein B 800-850 with the lipid environment and with the reaction center of the photosynthetic bacterium Rhodopseudomonas sphaeroides is studied by fluorescence spectroscopy, transient absorption techniques, light scattering and electron microscopy. Using vesicles of synthetic phospholipids it is shown that solidification of the m em brane causes a structural protein change evident from a reduction in fluorescence quantum yield. The change occurs at a tem perature up to 5 °C below that corresponding to the gel/fluid transition tem perature and indicates local melting. The structural change is not specific for the lipid head group nor chain length (investigated for lengths of 12 to 16 CH2 groups) and can be understood applying a simple elastic model. It can also be included isothermally by changing the ionic milied and thus varying the lipid phase state. Energy transfer LHCP→RC is proven to be highly efficient in model m em branes and is not affected by the existence of a phase transition. This indicates two LHCP fractions one tightly - and one non-bound to the RC.

scholarly journals Lipid droplet phase transition in freezing cat embryos and oocytes probed by Raman spectroscopy

2018 ◽  
Author(s):  
K.A. Okotrub ◽  
V.I. Mokrousova ◽  
S.Y. Amstislavsky ◽  
N.V. Surovtsev
Keyword(s):  
Phase Transition ◽  
Raman Spectroscopy ◽  
Lipid Droplets ◽  
Phase State ◽  
Domestic Cat ◽  
Lipid Phase ◽  
Lipid Phase Transition ◽  
Lipid Molecule ◽  
Early Embryos ◽  
Lipid Unsaturation

ABSTRACTEmbryo and oocyte cryopreservation is a widely used technology for cryopreservation of genetic resources. One challenging limitation of this technology is the cell damage during freezing associated with the intracellular lipid droplets. We exploit a Raman spectroscopy to investigate the freezing of cumulus-oocyte complexes, mature oocytes and early embryos of a domestic cat. All these cells are rich in lipids. The degree of lipid unsaturation, lipid phase transition from liquid-like disordered to solid-like ordered state (main transition) and triglyceride polymorphic state are studied. For all cells examined, the average degree of lipid unsaturation is estimated about 1.3 (with ±20 % deviation) double bonds per acyl chain. The onset of the main lipid phase transition occurs in a temperature range from −10 to +4 °C and does not depend significantly on the cell type. It is found that lipid droplets in cumulus-oocyte complexes undergo an abrupt lipid crystallization, which not completely correlate with the ordering of lipid molecule acyl chains. In the case of mature oocytes and early embryos obtainedin vitrofrom cumulus-oocyte complexes, the lipid phase transition is broadened. In frozen state lipid droplets inside the cumulus-oocyte complexes have higher content of triglyceride polymorphic β and β′ phases (∼66%) than it is estimated for the mature oocytes and the early embryos (∼50%). For the first time, to our knowledge, temperature evolution of lipid droplets phase state is examined. Raman spectroscopy is proved as a prospective tool forin situmonitoring of lipid phase state in single embryo/oocyte during freezing.

scholarly journals The Biosurfactant β-Aescin: A Review on the Physico-Chemical Properties and Its Interaction with Lipid Model Membranes and Langmuir Monolayers

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 117 ◽  
Author(s):  
Ramsia Geisler ◽  
Carina Dargel ◽  
Thomas Hellweg
Keyword(s):  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Phase State ◽  
Chemical Properties ◽  
Langmuir Monolayers ◽  
Model Membranes ◽  
Biologically Active ◽  
Horse Chestnut ◽  
Lipid Phase ◽  
Gel Phase

This review discusses recent progress in physicochemical understanding of the action of the saponin β -aescin (also called β -escin), the biologically active component in the seeds of the horse chestnut tree Aesculus hippocastanum. β -Aescin is used in pharmacological and cosmetic applications showing strong surface activity. In this review, we outline the most important findings describing the behavior of β -aescin in solution (e.g., critical micelle concentration ( c m c ) and micelle shape) and special physicochemical properties of adsorbed β -aescin monolayers at the air–water and oil–water interface. Such monolayers were found to posses very special viscoelastic properties. The presentation of the experimental findings is complemented by discussing recent molecular dynamics simulations. These simulations do not only quantify the predominant interactions in adsorbed monolayers but also highlight the different behavior of neutral and ionized β -aescin molecules. The review concludes on the interaction of β -aescin with phospholipid model membranes in the form of bilayers and Langmuir monolayers. The interaction of β -aescin with lipid bilayers was found to strongly depend on its c m c . At concentrations below the c m c , membrane parameters are modified whereas above the c m c , complete solubilization of the bilayers occurs, depending on lipid phase state and concentration. In the presence of gel-phase phospholipids, discoidal bicelles form; these are tunable in size by composition. The phase behavior of β -aescin with lipid membranes can also be modified by addition of other molecules such as cholesterol or drug molecules. The lipid phase state also determines the penetration rate of β -aescin molecules into lipid monolayers. The strongest interaction was always found in the presence of gel-phase phospholipid molecules.

In Situ Monitoring of Lipid Phase State Make Target Lipid Mixtures Similar to Intercellular Lipid in the Stratum Corneum

2020 ◽  
Vol 122 (3) ◽  
pp. 1900171
Author(s):  
Mika Yoshimura Fujii ◽  
Katsuhiko Gato ◽  
Yosuke Ozawa ◽  
Hiroshi Hisada ◽  
Tatsuo Koide ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Phase State ◽  
In Situ Monitoring ◽  
Lipid Phase ◽  
Lipid Mixtures ◽  
Intercellular Lipid

Effect of Lipid Phase State and Foam Film Type on the Properties of DMPG Stabilized Foams

1997 ◽  
Vol 190 (2) ◽  
pp. 278-285 ◽  
Author(s):  
Z.I. Lalchev ◽  
P.J. Wilde ◽  
D.C. Clark
Keyword(s):  
Phase State ◽  
Lipid Phase ◽  
Film Type ◽  
Foam Film

scholarly journals The Alignment of Membrane-Active Peptides Depends on the Lipid Phase State as Viewed by solid state 19F-NMR

2009 ◽  
Vol 96 (3) ◽  
pp. 156a
Author(s):  
Sergii Afonin ◽  
Stephan L. Grage ◽  
Marco Ieronimo ◽  
Daniel Maisch ◽  
Parvesh Wadhwani ◽  
...  
Keyword(s):  
Solid State ◽  
Phase State ◽  
19F Nmr ◽  
Lipid Phase ◽  
Active Peptides ◽  
Membrane Active Peptides
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