Purification and Characterization of Bone-Specific Alkaline Phosphatase from a Human Osteosarcoma Cell Line

1998 ◽  
Vol 62 (1) ◽  
pp. 67-73 ◽  
Author(s):  
M. Nakayama ◽  
I. Gorai ◽  
H. Minaguchi ◽  
C. Rosenquist ◽  
P. Qvist
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Line ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Purification And Characterization ◽  
Specific Alkaline Phosphatase ◽  
Bone Specific Alkaline Phosphatase ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cell

scholarly journals Effect of Orento, a Traditional Japanese Medicine, on IL-6, IL-8 Secretion, Type 1 Collagen Production and Alkaline Phosphatase Secretion in the Human Osteosarcoma Cell Line Saos-2

Medicines ◽  
2020 ◽  
Vol 7 (10) ◽  
pp. 61
Author(s):  
Hourei Oh ◽  
Kazuya Masuno ◽  
Nobutaka Okusa ◽  
Yoshimasa Makita ◽  
Shin-ichi Fujiwara ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Collagen Production ◽  
Human Osteosarcoma ◽  
Traditional Japanese Medicine ◽  
Human Osteosarcoma Cell

Background: Orento, a traditional Japanese medicine, is known as Kampo medicine in Japan. We investigated the possible efficacy of Kampo medicine for periodontal disease. In this study, we examined the in vitro effects of orento on the proliferation of the inflammatory cytokines interleukin (IL)-6 and IL-8, the production of type 1 collagen, and the secretion of alkaline phosphatase (ALP) in the human osteosarcoma cell line Saos-2 (Saos-2 cells). Methods: The proliferation of Saos-2 cells was assessed by MTT assay. IL-6 and IL-8 levels, type 1 collagen production and ALP secretion were evaluated using enzyme-linked immunosorbent assay and ALP assays. Saos-2 cells were treated with or without 0.1, 1, 10, 100 and 1000 μg/mL of orento for 24 h. Results: Orento (10 μg/mL) significantly induced the proliferation of Saos-2 cells. At this concentration, orento suppressed IL-6 and IL-8 and enhanced type 1 collagen production and ALP secretion. Conclusions: These results indicate that orento controls the IL-6 and IL-8 secretion and cellular metabolism of osteoblasts, resulting in the secretion of early bone-related biomarkers.

Comparison of Mechanosensitivity of Human Primary-Cultured Osteoblast Cells and Human Osteosarcoma Cell Line Under Hydrostatic Pressure

2012 ◽  
Author(s):  
Yasaman Ganji ◽  
Mehran Kasra
Keyword(s):  
Alkaline Phosphatase ◽  
Hydrostatic Pressure ◽  
Cell Line ◽  
Cell Model ◽  
Bone Cell ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Osteoblast Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cell

Regarding to the advances in mechanical stimulation of cells, this study aims to address important issues in bone generation and therapy at cellular level as it relates to understanding of bone cell response to hydrostatic pressure as well as choosing a proper cell model in studies of bone cell response to mechanical stimulation. G292 human osteosarcoma cell line and human primary osteoblast cells were tested under cyclic hydrostatic pressure. Monolayer culture of cells were divided into three groups of control without loading, static with the pressure of 0.5 MPa, and dynamic with the pressure of 0.5 MPa and the frequency of 1 Hz. The cells were analyzed with measurement of alkaline phosphatase activity. Higher level of alkaline phosphatase activity in all groups of primary cell model compared with cell line model indicated more sensitivity of response of primary cells to this kind of loading.

Characterization of newly established human osteosarcoma cell line, LM-1

In Vitro ◽  
1981 ◽  
Vol 17 (4) ◽  
pp. 308-314 ◽  
Author(s):  
Kwong-Y. Tsang ◽  
G. Shashidhar Pai ◽  
H. Hugh Fudenberg
Keyword(s):  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cell

scholarly journals Establishment and characterization of a cisplatin-resistant human osteosarcoma cell line

2014 ◽  
Vol 32 (3) ◽  
pp. 1133-1139 ◽  
Author(s):  
TAO HAN ◽  
XIAOMING ZHU ◽  
JULEI WANG ◽  
HAIEN ZHAO ◽  
QIONG MA ◽  
...  
Keyword(s):  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cell
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