Detection of 8-hydroxydeoxyguanosine, a marker of oxidative DNA damage, in white blood cells of workers occupationally exposed to styrene

1997 ◽  
Vol 71 (8) ◽  
pp. 496-500 ◽  
Author(s):  
B. Marczynski ◽  
P. Rozynek ◽  
H.-J. Elliehausen ◽  
M. Korn ◽  
X. Baur
Keyword(s):  
Dna Damage ◽  
Blood Cells ◽  
Oxidative Dna Damage ◽  
White Blood Cells ◽  
Occupationally Exposed

scholarly journals Healthcare Workers Occupationally Exposed to Ionizing Radiation Exhibit Altered Levels of Inflammatory Cytokines and Redox Parameters

Antioxidants ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 12 ◽  
Author(s):  
Iman M. Ahmad ◽  
Maher Y. Abdalla ◽  
Tiffany A. Moore ◽  
Lisa Bartenhagen ◽  
Adam J. Case ◽  
...  
Keyword(s):  
Dna Damage ◽  
Ionizing Radiation ◽  
Healthcare Workers ◽  
Immune Modulation ◽  
Oxidative Dna Damage ◽  
Underlying Mechanism ◽  
Increased Risk ◽  
Exposed Workers ◽  
Immune Alterations ◽  
Occupationally Exposed

Studies have shown an increased risk for a variety of cancers, specifically brain cancer, in healthcare workers occupationally exposed to ionizing radiation. Although the mechanisms mediating these phenomena are not fully understood, ionizing radiation-mediated elevated levels of reactive oxygen species (ROS), oxidative DNA damage, and immune modulation are likely involved. A group of 20 radiation exposed workers and 40 sex- and age-matched non-exposed control subjects were recruited for the study. We measured superoxide (O2•−) levels in whole blood of healthcare workers and all other measurements of cytokines, oxidative DNA damage, extracellular superoxide dismutase (EcSOD) activity and reduced/oxidized glutathione ratio (GSH/GSSG) in plasma. Levels of O2•− were significantly higher in radiation exposed workers compared to control. Similarly, a significant increase in the levels of interleukin (IL)-6, IL-1α and macrophage inflammatory protein (MIP)-1α in radiation exposed workers compared to control was observed, while there was no significance difference in the other 27 screened cytokines. A significant positive correlation was found between MIP-1α and O2•− levels with no correlation in either IL-6 or IL-1α. Further, a dose-dependent relationship with significant O2•− production and immune alterations in radiation exposed workers was demonstrated. There was no statistical difference between the groups in terms of oxidative DNA damage, GSH/GSSG levels, or EcSOD activity. Although the biologic significance of cytokines alterations in radiation exposed workers is unclear, further studies are needed for determining the underlying mechanism of their elevation.

Vitamin C supplementation decreases oxidative DNA damage in mononuclear blood cells of smokers

2004 ◽  
Vol 43 (5) ◽  
pp. 267-274 ◽  
Author(s):  
Peter M�ller ◽  
Michael Viscovich ◽  
Jens Lykkesfeldt ◽  
Steffen Loft ◽  
Annie Jensen ◽  
...  
Keyword(s):  
Dna Damage ◽  
Vitamin C ◽  
Blood Cells ◽  
Oxidative Dna Damage ◽  
Vitamin C Supplementation ◽  
Mononuclear Blood Cells

scholarly journals ROS-Induced DNA Damage Associates with Abundance of Mitochondrial DNA in White Blood Cells of the Untreated Schizophrenic Patients

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
I. V. Chestkov ◽  
E. M. Jestkova ◽  
E. S. Ershova ◽  
V. G. Golimbet ◽  
T. V. Lezheiko ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Paranoid Schizophrenia ◽  
Venous Blood ◽  
White Blood Cells ◽  
Mtdna Copy Number ◽  
Antipsychotic Medications ◽  
Significant Difference ◽  
Schizophrenic Patients ◽  
Male Patients

Objective. The aim of this study was (1) to examine the leukocyte mtDNA copy number (CN) in unmedicated (SZ (m−)) and medicated (SZ (m+)) male patients with paranoid schizophrenia (SZ) in comparison with the healthy male controls (HC) and (2) to compare the leukocyte mtDNA CN with the content of an oxidation marker 8-oxodG in lymphocytes of the SZ (m−) patients. Methods. We evaluated leukocyte mtDNA CN of 110 subjects with SZ in comparison with 60 male HC by the method qPCR (ratio mtDNA/nDNA (gene B2M) was detected). SZ patients were divided into two subgroups. The patients of the subgroups SZ (m+) (N=55) were treated with standard antipsychotic medications in the hospital. The patients of the subgroup SZ (m−) (N=55) were not treated before venous blood was sampled. To evaluate oxidative DNA damage, we quantified the levels of 8-oxodG in lymphocytes (flow cytometry) of SZ (m−) patients (N=55) and HC (N=30). Results. The leukocyte mtDNA CN showed no significant difference in SZ (m+) patients and HC. The mtDNA CN in the unmedicated subgroup SZ (m−) was significantly higher than that in the SZ (m+) subgroup or in HC group. The level of 8-oxodG in the subgroup SZ (m−) was significantly higher than that in HC group. Conclusion. The leukocytes of the unmedicated SZ male patients with acute psychosis contain more mtDNA than the leukocytes of the male SZ patients treated with antipsychotic medications or the healthy controls. MtDNA content positively correlates with the level of 8-oxodG in the unmedicated SZ patients.

The effects of strawberry tree water leaf extract, arbutin and hydroquinone on haematological parameters and levels of primary DNA damage in white blood cells of rats

2018 ◽  
Vol 215 ◽  
pp. 83-90
Author(s):  
Karlo Jurica ◽  
Irena Brčić Karačonji ◽  
Nevenka Kopjar ◽  
Ana Shek-Vugrovečki ◽  
Tihana Cikač ◽  
...  
Keyword(s):  
Dna Damage ◽  
Blood Cells ◽  
Leaf Extract ◽  
White Blood Cells ◽  
Haematological Parameters ◽  
Strawberry Tree ◽  
Primary Dna Damage

DNA damage in human white blood cells after inhalative exposure to methylenediphenyl diisocyanate (MDI) — case report

1992 ◽  
Vol 60 (2) ◽  
pp. 131-138 ◽  
Author(s):  
Boleslaw Marczynski ◽  
Adam B. Czuppon ◽  
Hans-Peter Hoffarth ◽  
Wolfgang Marek ◽  
Xaver Baur
Keyword(s):  
Dna Damage ◽  
Case Report ◽  
Blood Cells ◽  
White Blood Cells ◽  
Methylenediphenyl Diisocyanate ◽  
Human White Blood Cells ◽  
Inhalative Exposure

scholarly journals DNA Damaging Effects, Oxidative Stress Responses and Cholinesterase Activity in Blood and Brain of Wistar Rats Exposed to Δ9-Tetrahydrocannabinol

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1560 ◽  
Author(s):  
Nevenka Kopjar ◽  
Nino Fuchs ◽  
Suzana Žunec ◽  
Anja Mikolić ◽  
Vedran Micek ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Comet Assay ◽  
Stress Responses ◽  
Blood Cells ◽  
Genome Stability ◽  
White Blood Cells ◽  
Brain Cells ◽  
Alkaline Comet Assay

Currently we are faced with an ever-growing use of Δ9-tetrahydrocannabinol (THC) preparations, often used as supportive therapies for various malignancies and neurological disorders. As some of illegally distributed forms of such preparations, like cannabis oils and butane hash oil, might contain over 80% of THC, their consumers can become intoxicated or experience various detrimental effects. This fact motivated us for the assessments of THC toxicity in vivo on a Wistar rat model, at a daily oral dose of 7 mg/kg which is comparable to those found in illicit preparations. The main objective of the present study was to establish the magnitude and dynamics of DNA breakage associated with THC exposure in white blood and brain cells of treated rats using the alkaline comet assay. The extent of oxidative stress after acute 24 h exposure to THC was also determined as well as changes in activities of plasma and brain cholinesterases (ChE) in THC-treated and control rats. The DNA of brain cells was more prone to breakage after THC treatment compared to DNA in white blood cells. Even though DNA damage quantified by the alkaline comet assay is subject to repair, its elevated level detected in the brain cells of THC-treated rats was reason for concern. Since neurons do not proliferate, increased levels of DNA damage present threats to these cells in terms of both viability and genome stability, while inefficient DNA repair might lead to their progressive loss. The present study contributes to existing knowledge with evidence that acute exposure to a high THC dose led to low-level DNA damage in white blood cells and brain cells of rats and induced oxidative stress in brain, but did not disturb ChE activities.

scholarly journals The Effect of Selenium Supplementation in the Prevention of DNA Damage in White Blood Cells of Hemodialyzed Patients: A Pilot Study

2010 ◽  
Vol 142 (3) ◽  
pp. 274-283 ◽  
Author(s):  
Bronislaw A. Zachara ◽  
Jolanta Gromadzinska ◽  
Jadwiga Palus ◽  
Zbigniew Zbrog ◽  
Rafal Swiech ◽  
...  
Keyword(s):  
Dna Damage ◽  
Pilot Study ◽  
Blood Cells ◽  
White Blood Cells ◽  
Selenium Supplementation
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