Regulation of poly(β-hydroxybutyrate) synthesis in Methylobacterium rhodesianum MB 126 growing on methanol or fructose

1998 ◽  
Vol 169 (4) ◽  
pp. 360-363 ◽  
Author(s):  
G. Mothes ◽  
Jörg-Uwe Ackermann ◽  
Wolfgang Babel
Keyword(s):  
Methylobacterium Rhodesianum

Methylobacterium rhodesianum cells tend to double the DNA content under growth limitations and accumulate PHB

1995 ◽  
Vol 39 (1) ◽  
pp. 9-20 ◽  
Author(s):  
Jörg-uwe Ackermann ◽  
Susann Müller ◽  
Andreas Lösche ◽  
Thomas Bley ◽  
wolfgang Babel
Keyword(s):  
Dna Content ◽  
Methylobacterium Rhodesianum

Methylobacterium rhodesianum MB 126 possesses two acetoacetyl-CoA reductases

1994 ◽  
Vol 161 (3) ◽  
pp. 277-280
Author(s):  
Gisela Mothes ◽  
Wolfgang Babel
Keyword(s):  
Methylobacterium Rhodesianum

Accumulation of poly(3-hydroxybutyric acid) and overproduction of exopolysaccharides in a mutant of a methylotrophic bacterium

1995 ◽  
Vol 41 (13) ◽  
pp. 55-59 ◽  
Author(s):  
Uta Breuer ◽  
Jörg-Uwe Ackermann ◽  
Wolfgang Babel
Keyword(s):  
Ammonium Phosphate ◽  
Sole Source ◽  
Chemical Mutagenesis ◽  
Methylotrophic Bacterium ◽  
Wild Type ◽  
Hydroxybutyric Acid ◽  
Serine Pathway ◽  
Methylobacterium Rhodesianum

The pink-pigmented facultatively methylotrophic bacterium Methylobacterium rhodesianum MB 126 is able to grow on methanol as the sole source of carbon and energy. Under certain conditions, e.g., limitation of ammonium, phosphate, or oxygen, carbon from methanol is channeled into poly(3-hydroxybutyric acid) (PHB) whereas other polymers or metabolites are hardly overproduced. A mutant of this strain, which we isolated after chemical mutagenesis, is impaired in its ability to synthesize PHB. Under the conditions mentioned above, the mutant still accumulated PHB, but in the absence of ammonium it simultaneously synthesized PHB and a considerable amount of an exopolysaccharide. This phenomenon was surprising insofar as the wild type did not produce exopolysaccharide in such amounts. An attempt was made to elucidate and discuss the possible reasons for these findings.Key words: methylotrophy, serine pathway bacteria, PHB, exopolysaccharides.

Methylobacterium rhodesianum MB?126 possesses two acetoacetyl-CoA reductases

1994 ◽  
Vol 161 (3) ◽  
pp. 277-280
Author(s):  
Gisela Mothes ◽  
Wolfgang Babel
Keyword(s):  
Methylobacterium Rhodesianum

scholarly journals Production of the Chiral Compound (R)-3-Hydroxybutyrate by a Genetically Engineered Methylotrophic Bacterium

2010 ◽  
Vol 76 (16) ◽  
pp. 5585-5591 ◽  
Author(s):  
Tina Hölscher ◽  
Uta Breuer ◽  
Lorenz Adrian ◽  
Hauke Harms ◽  
Thomas Maskow
Keyword(s):  
Citric Acid Cycle ◽  
Sole Source ◽  
Genetically Engineered ◽  
Methylotrophic Bacterium ◽  
Cultivation Conditions ◽  
Chiral Compound ◽  
Batch Cultures ◽  
Alternative Pathways ◽  
Intracellular Degradation ◽  
Methylobacterium Rhodesianum

ABSTRACT In this study, a methylotrophic bacterium, Methylobacterium rhodesianum MB 126, was used for the production of the chiral compound (R)-3-hydroxybutyrate (R-3HB) from methanol. R-3HB is formed during intracellular degradation of the storage polymer (R)-3-polyhydroxybutyrate (PHB). Since the monomer R-3HB does not accumulate under natural conditions, M. rhodesianum was genetically modified. The gene (hbd) encoding the R-3HB-degrading enzyme, R-3HB dehydrogenase, was inactivated in M. rhodesianum. The resulting hbd mutant still exhibited low growth rates on R-3HB as the sole source of carbon and energy, indicating the presence of alternative pathways for R-3HB utilization. Therefore, transposon mutagenesis was carried out with the hbd mutant, and a double mutant unable to grow on R-3HB was obtained. This mutant was shown to be defective in lipoic acid synthase (LipA), resulting in an incomplete citric acid cycle. Using the hbd lipA mutant, we produced 3.2 to 3.5 mM R-3HB in batch and 27 mM (2,800 mg liter−1) in fed-batch cultures. This was achieved by sequences of cultivation conditions initially favoring growth, then PHB accumulation, and finally PHB degradation.

Isolation and purification of granule-associated proteins relevant for poly(3-hydroxybutyric acid) biosynthesis from methylotrophic bacteria relying on the serine pathway

1995 ◽  
Vol 41 (13) ◽  
pp. 124-130 ◽  
Author(s):  
C. G. Föllner ◽  
W. Babel ◽  
A. Steinbüchel
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Methylotrophic Bacteria ◽  
Hydroxybutyric Acid ◽  
Isolation And Purification ◽  
Small Proteins ◽  
Associated Proteins ◽  
Serine Pathway ◽  
Methylobacterium Rhodesianum ◽  
Leaky Mutants

The poly(3-hydroxybutyric acid) (PHB) granules from eight methylotrophic bacteria that use the serine pathway were isolated in a sucrose gradient (1–2 M); these bacteria included members of the genus Methylobacterium, Mycoplana rubra, and PHB-leaky mutants of Methylobacterium rhodesianum. As shown by sodium dodecyl sulfate – polyacrylamide gel electrophoresis, the granules from all investigated methylotrophic strains revealed two major bands representing small proteins. An efficient purification procedure for these two low molecular weight proteins associated with the PHB granules was developed by solubilization of the proteins with Triton X-114 and affinity chromatography on Procion Blue-H-ERD.Key words: poly(3-hydroxybutyric acid), granule-associated proteins, methylotrophic bacteria.

Effective utilization of dichloromethane by a newly isolated strain Methylobacterium rhodesianum H13

2013 ◽  
Vol 21 (2) ◽  
pp. 1010-1019 ◽  
Author(s):  
Dong-Zhi Chen ◽  
Du-Juan Ouyang ◽  
Hong-Xia Liu ◽  
Jing Chen ◽  
Qing-Feng Zhuang ◽  
...  
Keyword(s):  
Effective Utilization ◽  
Methylobacterium Rhodesianum
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