Identification of QTL involved in field resistance to light leaf spot (Pyrenopeziza brassicae) and blackleg resistance (Leptosphaeria maculans) in winter rapeseed (Brassica napus L.)

1998 ◽  
Vol 97 (3) ◽  
pp. 398-406 ◽  
Author(s):  
M. L. Pilet ◽  
R. Delourme ◽  
N. Foisset ◽  
M. Renard
1995 ◽  
Vol 75 (2) ◽  
pp. 437-439 ◽  
Author(s):  
G. R. Stringam ◽  
V. K. Bansal ◽  
M. R. Thiagarajah ◽  
D. F. Degenhardt ◽  
J. P. Tewari

The doubled haploid breeding method and greenhouse screening using cotyledon bio-assay were successfully applied to transfer blackleg resistance from the Australian cultivar Maluka (Brassicas napus), into susceptible advanced B. napus lines from the University of Alberta. This approach for blackleg resistance breeding was effective and efficient as several superior blackleg resistant breeding lines were identified within 4 yr from the initial cross. One of these lines (91–21864NA) was entered in the 1993 trials of the Western Canada Canola/Rapeseed Recommending Committee. Key words: Blackleg resistance, Leptosphaeria maculans, doubled haploid, Brassica napus


2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 572-574
Author(s):  
E. Plachká ◽  
L. Odstrčilová

The pure cultures of the fungus Leptosphaeria maculans were isolated from the rapeseed leaves collected in Moravia. Two isolates were collected on the basis of their ability to produce yellow colour. The isolate producing yellow colour was classified as aggressive, while the isolate without the ability to produce yellow colour was not aggressive. The sensitivity to these isolates was verified in 11 rapeseed genotypes. This test was based on the method from the document Crucifer Genetics Cooperative (information document from the Department of Plant Pathology, University of Wisconsin). This method is based on the inoculation of cruciferous leaflets with suspension from the isolate of the pathogen observed. The scale from 0 to 9 was used for evaluation (0 = no darkening around the wound, 9 = accompanied by profuse sporulation in large, more than 5 mm, lesions with diffuse margins). The infection degree of the aggressive isolate ranged in different genotypes from 1.45 to 4.5, the results of the non-aggressive isolate ranged from 1.0 to 1.5. Based on these results the aggressive isolate was used for further tests of the selected breeding lines. The tests involved selected breeding materials, registered varieties, control varieties with different sensitivity to the isolate of Leptosphaeria maculans and control varieties included in state trials. The maximal difference in the sensitivity exhibited by individual genotypes was five degrees.


2020 ◽  
Vol 22 (1) ◽  
pp. 313
Author(s):  
Aldrin Y. Cantila ◽  
Nur Shuhadah Mohd Saad ◽  
Junrey C. Amas ◽  
David Edwards ◽  
Jacqueline Batley

Among the Brassica oilseeds, canola (Brassica napus) is the most economically significant globally. However, its production can be limited by blackleg disease, caused by the fungal pathogen Lepstosphaeria maculans. The deployment of resistance genes has been implemented as one of the key strategies to manage the disease. Genetic resistance against blackleg comes in two forms: qualitative resistance, controlled by a single, major resistance gene (R gene), and quantitative resistance (QR), controlled by numerous, small effect loci. R-gene-mediated blackleg resistance has been extensively studied, wherein several genomic regions harbouring R genes against L. maculans have been identified and three of these genes were cloned. These studies advance our understanding of the mechanism of R gene and pathogen avirulence (Avr) gene interaction. Notably, these studies revealed a more complex interaction than originally thought. Advances in genomics help unravel these complexities, providing insights into the genes and genetic factors towards improving blackleg resistance. Here, we aim to discuss the existing R-gene-mediated resistance, make a summary of candidate R genes against the disease, and emphasise the role of players involved in the pathogenicity and resistance. The comprehensive result will allow breeders to improve resistance to L. maculans, thereby increasing yield.


2009 ◽  
Vol 120 (5) ◽  
pp. 921-931 ◽  
Author(s):  
Wolfgang Ecke ◽  
Rosemarie Clemens ◽  
Nora Honsdorf ◽  
Heiko C. Becker

1986 ◽  
Vol 66 (3) ◽  
pp. 425-430 ◽  
Author(s):  
R. R. DUNCAN ◽  
C. S. HOVELAND

Since rainfall generally exceeds 500 mm during the winter months and soil erosion is a problem in the southern U.S.A., a cover crop is essential to reduce soil losses. Continuous cultivation of a crop on the same land also provides the opportunity for pest problems to develop. A winter rapeseed (Brassica napus L.)-grain sorghum (Sorghum bicolor (L). Moench.) double-cropping system was investigated in the field from 1981 to 1984. Conventional tillage (CT) and no-till (NT) systems were used for both crops. Gullivar rape was planted in October and harvested in June. Winter rapeseed yields averaged 2.87 t ha−1 on CT plots in 1982. Averaged over 1983 and 1984, rape yields were 1.66 and 0.73 t ha−1 on CT and NT plots, respectively. However, the seeding method biased the rapeseed performance. Problems were encountered with winter weed control and pod shattering in the winter rape plots. Two sorghum hybrids, R. C. Young Oro Txtra and Funks G-550, were planted in June and harvested in October. Grain yields were not significantly different between hybrids (Oro 5.69 t ha−1, Funk 5.67 t ha−1) or between tillage systems (CT 5.59 t ha−1, NT 5.78 t ha−1). Grain yield differences were noted for year × hybrid × tillage interactions. Rape was a weed problem in the sorghum plots. The winter rape-sorghum double-cropping system offers an alternative to current winter small grains-sorghum/soybean (Glycine max (L.) Merr.) or winter annual legume-sorghum/soybean (Glycine max (L.) Merr.) systems; however, markets for the winter rapeseed and production economic analyses are needed prior to adoption of the system. Additional winter rapeseed research is needed to evaluate yields properly under NT conditions.Key words: Sorghum bicolor (L.) Moench, Brassica napus L., conservation tillage, multiple cropping, sorghum, rapeseed


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