Identification and molecular characterization of a large insertion within the repetitive domain of a high-molecular-weight glutenin subunit gene from hexaploid wheat

1996 ◽  
Vol 93 (7) ◽  
pp. 1048-1053 ◽  
Author(s):  
R. D'Ovidio ◽  
D. Lafiandra ◽  
E. Porceddu
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Molecular Characterization ◽  
Hexaploid Wheat ◽  
Glutenin Subunit ◽  
Subunit Gene ◽  
Repetitive Domain ◽  
Large Insertion

scholarly journals Characterisation of a novel high-molecular-weight glutenin subunit 1Dy12.3 from hexaploid wheat (Triticum aestivum L.)

2012 ◽  
Vol 48 (No. 4) ◽  
pp. 157-168 ◽  
Author(s):  
D. Mihálik ◽  
E. Gregová ◽  
P. Galuszka ◽  
L. Ohnoutková ◽  
T. Klempová ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Triticum Aestivum ◽  
High Molecular Weight ◽  
Hexaploid Wheat ◽  
Signal Sequence ◽  
Triticum Aestivum L ◽  
Glutenin Subunit ◽  
Repetitive Domain ◽  
Sds Page ◽  
Methionine Residues

A novel high-molecular-weight glutenin subunit encoded by the Glu-1D locus was identified in hexaploid wheat (Triticum aestivum L.) cultivar Noe and was designated as 1Dy12.3. This subunit differed in SDS-PAGE mobility from the well-known 1Dy10 and 1Dy12 subunits that are also encoded by this locus. An analysis of the gene sequences confirmed the uniqueness of 1Dy12.3 and revealed that it is most closely related to the 1Dy12 subunit. The size of the deduced protein was calculated to be 67 884 Da, which is different from the 1Dy10 and 1Dy12 subunits (67 475 Da and 68 713 Da, respectively). The 1Dy12.3 protein consists of 652 residues, with a highly conserved signal sequence and N- and C-terminal domains, although the central repetitive domain comprising motifs of hexapeptide (PGQGQQ) and nonapeptide (GYYPTSLQQ) repeats was less conserved. The 1Dy12.3 subunit demonstrates fewer QHPEQG hexapeptide motifs and exhibits an increased number of methionine residues in comparison to the other characterised high-molecular-weight glutenin subunits. The 1Dy12.3 subunit was cloned and expressed in Escherichia coli and was detected with a prolamin-specific antibody. The size of the detected immunocomplex corresponded to the native 1Dy12.3 protein isolated from grains. The existence and characterisation of this novel high-molecular-weight glutenin subunit increases the diversity of the glutenins encoded by the Glu-1D locus.

scholarly journals Molecular Characterization of Novel x-Type HMW Glutenin Subunit 1B × 6.5 in Wheat

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2108
Author(s):  
Tímea Kuťka Hlozáková ◽  
Zdenka Gálová ◽  
Svetlana Šliková ◽  
Leona Leišová-Svobodová ◽  
Jana Beinhauer ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Molecular Characterization ◽  
Glutenin Subunit ◽  
Specific Primers ◽  
Sds Page ◽  
Hmw Glutenin ◽  
Sequence Encoding ◽  
High Level

A novel high molecular weight glutenin subunit encoded by the Glu-1B locus was identified in the French genotype Bagou, which we named 1B × 6.5. This subunit differed in SDS-PAGE from well-known 1B × 6 and 1B × 7 subunits, which are also encoded at this locus. Subunit 1B × 6.5 has a theoretical molecular weight of 88,322.83 Da, which is more mobile than 1B × 6 subunit, and isoelectric point (pI) of about 8.7, which is lower than that for 1B × 6 subunit. The specific primers were designed to amplify and sequence 2476 bp of the Glu-1B locus from genotype Bagou. A high level of similarity was found between the sequence encoding 1B × 6.5 and other x-type encoding alleles of this locus.

scholarly journals Molecular characterization of high molecular weight glutenin subunit allele 1Bx23 in common wheat introduced from hexaploid triticale

Hereditas ◽  
2006 ◽  
Vol 143 (2006) ◽  
pp. 159-166 ◽  
Author(s):  
ZU-JUN YANG ◽  
GUANG-RONG LI ◽  
HUAN-LIN SHU ◽  
CHANG LIU ◽  
JUAN FENG ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Molecular Characterization ◽  
Common Wheat ◽  
Glutenin Subunit ◽  
Hexaploid Triticale
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