Isolation and Characterization of a Carbonic Anhydrase Homologue from the Zebrafish (Danio rerio)

1997 ◽  
Vol 44 (4) ◽  
pp. 432-439 ◽  
Author(s):  
Robert E. Peterson ◽  
Chingkuang Tu ◽  
Paul J. Linser
Keyword(s):  
Carbonic Anhydrase ◽  
Danio Rerio ◽  
Isolation And Characterization

The mouse carbonic anhydrase I gene contains two tissue-specific promoters

1989 ◽  
Vol 9 (8) ◽  
pp. 3308-3313
Author(s):  
P Fraser ◽  
P Cummings ◽  
P Curtis
Keyword(s):  
Carbonic Anhydrase ◽  
Splice Acceptor Site ◽  
Acceptor Site ◽  
Exon 2 ◽  
Tissue Specific ◽  
Isolation And Characterization ◽  
Carbonic Anhydrase I ◽  
Erythroleukemia Cells ◽  
Exon 1

We report the isolation and characterization of the mouse carbonic anhydrase I (CAI) gene. Direct RNA sequence analysis of the 5' nontranslated regions of CAI mRNA from mouse colon and mouse erythroleukemia cells demonstrated tissue specificity in the lengths and sequences of CAI transcripts. Analysis of several mouse CAI genomic clones showed that the transcripts arose from a single CAI gene with two tissue-specific promoters and eight exons. CAI transcripts in the colon were found to initiate just upstream of the erythroid exon 2 of the CAI gene region sequence. Erythroid transcripts originated from a novel promoter upstream of exon 1, which was located more than 10 but less than 250 kilobases upstream of exon 2. Erythroid exon 1 contained only a nontranslated sequence, which was spliced to exon 2 via a cryptic splice acceptor site located in the region that encoded the colon mRNA 5' nontranslated sequence. The remaining exon-intron junctions were conserved in comparison with those of the CAII and CAIII genes.

scholarly journals Identification, Isolation and Origin of Potential Dimer Impurities of Dichlorphenamide: A Carbonic Anhydrase Inhibitor Drug (Antiglaucoma)

2021 ◽  
Vol 33 (6) ◽  
pp. 1249-1252
Author(s):  
T. Purendar Reddy ◽  
Nageshwar Dussa ◽  
Srinivas Mamidi ◽  
Mahesh Panasa ◽  
Ramadas Chavakula ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Drug Product ◽  
Carbonic Anhydrase Inhibitor ◽  
Drug Substance ◽  
Substantial Impact ◽  
Isolation And Characterization ◽  
New Process ◽  
Inhibitor Drug

Present work describes the identification, isolation and characterization of two new process related dimeric impurities of dichlorphenamide (1). During the synthesis of dichlorphenamide, the formation of two new dimeric impurities namely 2,3-dichloro-5-(3,4-dichloro-5-sulfamoyl-phenyl)sulfonylbenzenesulfonamide (2) (1,1Œ-dichlorphenamide dimer) and 2,3-dichloro-5-(2,3-dichloro-5-sulfamoylphenyl) sulfonyl-benzenesulfonamide (3) (1,3Œ-dichlorphenamide dimer) was observed. In addition, origin and the strategies adapted to control these potential dimer impurities were also described. These impurities have a substantial impact on the quality of the drug substance as well as drug product.

scholarly journals Isolation and characterization of monoclonal antibodies against human carbonic anhydrase-IX

mAbs ◽  
2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Anne E.G. Lenferink ◽  
Paul C. McDonald ◽  
Christiane Cantin ◽  
Suzanne Grothé ◽  
Mylene Gosselin ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Carbonic Anhydrase ◽  
Carbonic Anhydrase Ix ◽  
Isolation And Characterization ◽  
Human Carbonic Anhydrase

Isolation and characterization of LIGHT (TNFSF14) gene homologue in zebrafish (Danio rerio)

2012 ◽  
Vol 14 (4) ◽  
pp. 629-634 ◽  
Author(s):  
Ai-ying Tian ◽  
Hui-juan Yang ◽  
Shi-cheng Zhu ◽  
Yu-shun Zhang ◽  
Zheng-an Jiang ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Isolation And Characterization ◽  
Gene Homologue
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