Interaction between acridine orange and sulphated polysaccharides

2007 ◽  
pp. 141-144 ◽  
Author(s):  
H. Watanabe ◽  
K. Nishida
Keyword(s):  
Acridine Orange ◽  
Sulphated Polysaccharides

Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

1990 ◽  
Vol 48 (3) ◽  
pp. 168-169
Author(s):  
M. H. Chestnut ◽  
C. E. Catrenich
Keyword(s):  
Acridine Orange ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ulcer Disease ◽  
Gastroduodenal Disease ◽  
H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

Frequent Occurrence of HIV-Inhibitory Sulphated Polysaccharides in Marine Invertebrates

1993 ◽  
Vol 4 (3) ◽  
pp. 167-172 ◽  
Author(s):  
J. A. Beutler ◽  
T. C. McKee ◽  
R. W. Fuller ◽  
M. Tischler ◽  
J. H. Cardellina ◽  
...  
Keyword(s):  
Marine Invertebrates ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Size Exclusion ◽  
Aqueous Extracts ◽  
Sulphated Polysaccharides ◽  
Anionic Polysaccharides ◽  
Gel Permeation ◽  
Anti Hiv ◽  
C Nmr

Aqueous extracts of many marine invertebrates have exhibited some activity in the National Cancer Institute's primary screen for anti-HIV cytopathicity. Using a variety of techniques, including gel permeation, size exclusion and ion exchange chromatography, toluidine blue metachromicity, 13C-NMR spectroscopy and combustion analyses, we have determined that this activity is largely due to sulphated polysaccharides. Because of the wide occurrence of this class of compounds in these organisms we sought a method for the rapid dereplication of sulphated polysaccharides. It was critical that the method selected for dereplication allow differentiation of anionic polysaccharides from other AIDS-antiviral chemotypes. After evaluating a variety of methods, we found that the most efficient strategy appeared to be precipitation of the polysaccharide fraction from aqueous ethanolic solutions of the crude aqueous extracts.

scholarly journals A Turn-ON fluorometric biosensor based on ssDNA immobilized with a metal phenolic nanomaterial for the sequential detection of Pb(ii) and epirubicin cancer drug

RSC Advances ◽  
2021 ◽  
Vol 11 (20) ◽  
pp. 12361-12373
Author(s):  
A. Arunjegan ◽  
P. Rajaji ◽  
S. Sivanesan ◽  
P. Panneerselvam
Keyword(s):  
Acridine Orange ◽  
Cancer Drug ◽  
Sequential Detection ◽  
Label Free ◽  
Fluorescent Biosensor ◽  
Turn On

In this paper, we propose a fluorescent biosensor for the sequential detection of Pb2+ ions and the cancer drug epirubicin (Epn) using the interactions between label-free guanine-rich ssDNA (LFGr-ssDNA), acridine orange (AO), and a metal–phenolic nanomaterial.

Multimode binding and stimuli responsive displacement of acridine orange dye complexed with p-sulfonatocalix[4/6]arene macrocycles

2020 ◽  
Vol 22 (23) ◽  
pp. 13306-13319
Author(s):  
Mhejabeen Sayed ◽  
Dona M. Tom ◽  
Haridas Pal
Keyword(s):  
Acridine Orange ◽  
Dynamic Quenching ◽  
Stimuli Responsive ◽  
Turn On ◽  
Pictorial Presentation ◽  
Fluorescence Turn On ◽  
Multi Mode

Pictorial presentation of the different aspects as displayed by the AOH+–SCXn systems in regard to multi-mode binding, dynamic quenching and stimuli responsive fluorescence “turn ON”, demonstrating very rich supramolecular photochemistry.

scholarly journals Radiobiological and dosimetric assessment of DNA-intercalated 99mTc-complexes bearing acridine orange derivatives

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ana Belchior ◽  
Salvatore Di Maria ◽  
Célia Fernandes ◽  
Pedro Vaz ◽  
António Paulo ◽  
...  
Keyword(s):  
Acridine Orange ◽  
Dosimetric Assessment

Acridine Orange as a Useful Chromosomal Stain

1962 ◽  
Vol 37 (6_ts) ◽  
pp. 669-670 ◽  
Author(s):  
Lewis M. Schiffer ◽  
Tiiu Vaharu
Keyword(s):  
Acridine Orange
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