Construction and characterisation of a hexaploid wheat (Triticum aestivum L.) BAC library from the reference germplasm ‘Chinese Spring’

2003 ◽  
Vol 31 (3-4) ◽  
pp. 331-338 ◽  
Author(s):  
S. Allouis ◽  
G. Moore ◽  
A. Bellec ◽  
R. Sharp ◽  
P. Faivre Rampant ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Hexaploid Wheat ◽  
Chinese Spring ◽  
Bac Library ◽  
Triticum Aestivum L

scholarly journals Genetic Analysis of Hexaploid Wheat (Triticum aestivum L.) Using the Complete Sequencing of Chloroplast DNA and Haplotype Analysis of the Wknox1 Gene

2021 ◽  
Vol 22 (23) ◽  
pp. 12723
Author(s):  
Mari Gogniashvili ◽  
Yoshihiro Matsuoka ◽  
Tengiz Beridze
Keyword(s):  
Triticum Aestivum ◽  
Chloroplast Dna ◽  
Hexaploid Wheat ◽  
Chinese Spring ◽  
Haplotype Analysis ◽  
Triticum Aestivum L ◽  
Three Samples ◽  
Two Samples ◽  
Complete Sequencing ◽  
Exon Region

The aim of the presented study is a genetic characterization of the hexaploid wheat Triticum aestivum L. Two approaches were used for the genealogical study of hexaploid wheats—the complete sequencing of chloroplast DNA and PCR-based haplotype analysis of the fourth intron of Wknox1d and of the fifth-to-sixth-exon region of Wknox1b. The complete chloroplast DNA sequences of 13 hexaploid wheat samples were determined: Free-threshing—T. aestivum subsp. aestivum, one sample; T. aestivum subsp. compactum, two samples; T. aestivum subsp. sphaerococcum, one sample; T. aestivum subsp. carthlicoides, four samples. Hulled—T. aestivum subsp. spelta, three samples; T. aestivum subsp. vavilovii jakubz., two samples. The comparative analysis of complete cpDNA sequences of 20 hexaploid wheat samples (13 samples in this article plus 7 samples sequenced in this laboratory in 2018) was carried out. PCR-based haplotype analysis of the fourth intron of Wknox1d and of the fifth-to-sixth exon region of Wknox1b of all 20 hexaploid wheat samples was carried out. The 20 hexaploid wheat samples (13 samples in this article plus 7 samples in 2018) can be divided into two groups—T. aestivum subsp. spelta, three samples and T. aestivum subsp. vavilovii collected in Armenia, and the remaining 16 samples, including T. aestivum subsp. vavilovii collected in Europe (Sweden). If we take the cpDNA of Chinese Spring as a reference, 25 SNPs can be identified. Furthermore, 13–14 SNPs can be identified in T. aestivum subsp. spelta and subsp. vavilovii (Vav1). In the other samples up to 11 SNPs were detected. 22 SNPs are found in the intergenic regions, 2 found in introns, and 10 SNPs were found in the genes, of which seven are synonymous. PCR-based haplotype analysis of the fourth intron of Wknox1d and the fifth-to-sixth-exon region of Wknox1b provides an opportunity to make an assumption that hexaploid wheats T. aestivum subsp. macha var. palaeocolchicum and var. letshckumicum differ from other macha samples by the absence of a 42 bp insertion in the fourth intron of Wknox1d. One possible explanation for this observation would be that two Aegilops tauschii Coss. (A) and (B) participated in the formation of hexaploids through the D genome: Ae. tauschii (A)—macha (1–5, 7, 8, 10–12), and Ae. tauschii (B)—macha M6, M9, T. aestivum subsp. aestivum cv. ‘Chinese Spring’ and cv. ‘Red Doly’.

Chromosomal localization of intergenomic RFLP loci in hexaploid wheat

Genome ◽  
1993 ◽  
Vol 36 (5) ◽  
pp. 913-918 ◽  
Author(s):  
Michael E. Devey ◽  
Gary E. Hart
Keyword(s):  
Triticum Aestivum ◽  
Genetic Mapping ◽  
Hexaploid Wheat ◽  
Chinese Spring ◽  
Genomic Dna ◽  
Chromosomal Localization ◽  
Triticum Aestivum L ◽  
Dna Probes ◽  
Dna Fragments ◽  
Homoeologous Chromosomes

Hybridization of radiolabeled wheat DNA probes to genomic DNA digests of compensating nullisomic-tetrasomic lines and ditelosomic lines of hexaploid wheat (Triticum aestivum L. cv. Chinese Spring) can be used to identify intergenomic RFLPs. Sixty-three PstI/BamHI genomic DNA probes and eight cDNA probes were used to determine the chromosomal locations of 223 DNA fragments that define a minimum of 189 RFLP loci. Eighty-four percent of the genomic DNA clones hybridize to fragments located in homoeologous chromosomes and 16% hybridize to fragments located in one chromosome only or to fragments located in nonhomoeologous chromosomes. All of the cDNA probes hybridize to fragments located in homoeologous chromosomes.Key words: aneuploids, genetic mapping.

scholarly journals CHROMOSOMAL LOCATION OF GENES CONTROLLING FLAVONOID PRODUCTION IN HEXAPLOID WHEAT

Genetics ◽  
1983 ◽  
Vol 103 (2) ◽  
pp. 313-321
Author(s):  
Paula R Neuman ◽  
J G Waines ◽  
K W Hilu ◽  
D Barnhart
Keyword(s):  
Triticum Aestivum ◽  
Hexaploid Wheat ◽  
Chinese Spring ◽  
Chromosomal Location ◽  
Triticum Aestivum L ◽  
Phenolic Glycoside ◽  
Two Dimensional ◽  
Chromosome Arm ◽  
The Individual ◽  
Homoeologous Chromosomes

ABSTRACT Two-dimensional paper chromatography was performed on methanol extracts of leaves of hexaploid bread wheat, Triticum aestivum L. em. Thell. cultivar Chinese Spring, and of the available nullisomic-tetrasomic compensating lines, the tetrasomic lines and the ditelocentric lines. The chromatograms had 27 spots identified as flavonoids and six representing phenolic acids. Some of the areas were complex and contained more than one compound. Four flavonoids were identified as under the control of gene(s) on chromosome arms 1DS, 4DL, 5AS and 6BS. A phenolic glycoside was concluded to be controlled by a gene(s) on chromosome arm 7BL. Gene(s) on chromosome arm 4DL affected the amount of compounds in two other spots, and gene(s) on chromosome arm 4BS reduced the level of all flavonoid compounds. The individual compounds in some of the complex spots may be under the control of gene(s) on homoeologous chromosomes.

Construction and characterization of a bacterial artificial chromosome (BAC) library of hexaploid wheat (Triticum aestivum L.) and validation of genome coverage using locus-specific primers

Genome ◽  
2003 ◽  
Vol 46 (5) ◽  
pp. 870-878 ◽  
Author(s):  
Sasanda D Nilmalgoda ◽  
Sylvie Cloutier ◽  
Andrzej Z Walichnowski
Keyword(s):  
Triticum Aestivum ◽  
Hexaploid Wheat ◽  
Bac Library ◽  
Triticum Aestivum L ◽  
Resistance Locus ◽  
Insert Size ◽  
Specific Primers ◽  
Hindiii Site ◽  
Genome Coverage ◽  
Hmw Glutenin

A BAC library of hexaploid wheat was constructed using the spring wheat cultivar Triticum aestivum L. 'Glenlea'. Fresh shoot tissue from 7- to 10-day-old seedlings was used to obtain HMW DNA. The library was constructed using the HindIII site of pIndigoBAC-5 and the BamHI site of pIndigoBAC-5 and pECBAC1. A total of 12 ligations were used to construct the entire library, which contains over 650 000 clones. Ninety-six percent of the clones had inserts. The insert size ranged from 5 to 189 kb with an average of 79 kb. The entire library was gridded onto 24 high-density filters using a 5 × 5 array. A subset of these membranes was hybridized with two intergenic chloroplast probes and the percentage of clones containing chloroplast DNA (cpDNA) was calculated to be 2.2%. The genome coverage was estimated to be 3.1 × haploid genome equivalents, giving a 95.3% probability of identifying a clone corresponding to any wheat DNA sequence. BAC pools were constructed and screened using markers targeting the Glu-B1 locus (1BL), the hardness loci (5AS, 5BS, 5DS), the leaf rust resistance locus Lr1 (5DL), and the major fusarium head blight QTL locus located on 3BS. These markers were either locus-specific amplicons or microsatellites. A total of 49 BAC clones were identified for 14 markers giving an average of 3.5 clones/marker, thereby corroborating the estimated 3.1× genome coverage. An example using the gene encoding the HMW glutenin Bx7 is illustrated.Key words: BAC library, BAC pools, hexaploid wheat, locus-specific primers, HMW glutenin.

Chilling injury and chlorotic reactions of euploids and aneuploids of the group 6 homoeologues of common wheat

Genome ◽  
1992 ◽  
Vol 35 (3) ◽  
pp. 468-473 ◽  
Author(s):  
Ernest D. P. Whelan ◽  
G. B. Schaalje
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Chinese Spring ◽  
Prolonged Exposure ◽  
Chilling Injury ◽  
Triticum Aestivum L ◽  
Recessive Trait ◽  
Cytoplasmic Effects ◽  
Group 6 ◽  
Winter Wheat Cultivars

Aneuploid seedlings of the common wheat (Triticum aestivum L.) cv. Chinese Spring (CS) that are nullisomic or telosomic for the long arm of chromosome 6D are susceptible to chilling injury under prolonged exposure to 6 °C; normal euploids or telosomics for the short arm are not. Studies of seedling grown for various durations at 20 °C prior to growth at 6 °C showed that chilling injury was a juvenile phenomenon and that the extent of injury was inversely proportional to the duration of growth at 20 °C to a maximum of about 14 days. When reciprocal crosses were made between susceptible 6D nullisomics or long-arm ditelocentrics of CS and resistant 6D nullisomics of three spring and one winter wheat cultivars, progenies from aneuploid F1 hybrids all segregated for susceptibility as a recessive trait and at a frequency approximating a dihybrid ratio; no cytoplasmic effects were detected. Aneuploids of the group 6 homoeologues of the spring wheat cvs. Cadet and Rescue were resistant, as were group 6 whole-chromosome substitutions of eight different donor wheats in the recipient parent CS and 56 other euploids tested. Genes for resistance to chilling injury appear to involve the group 6 chromosomes and the short arm of 6D in Chinese Spring. In contrast with chilling injury, all aneuploid lines with only four doses of the "corroded" loci on group 6 chromosomes exhibited chlorotic symptoms.Key words: Triticum aestivum, chilling injury.

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