Effects of the antileukemic drug L-asparaginase on sex hormone-binding globulin: studies in vivo and in vitro

1989 ◽  
Vol 12 (7) ◽  
pp. 489-493 ◽  
Author(s):  
L. Bartalena ◽  
E. Martino ◽  
A. Pacchiarotti ◽  
S. Balzano ◽  
M. Falcone ◽  
...  
Keyword(s):  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding

A COMPARISON OF THE EFFECTS OF DANAZOL AND GESTRINONE ON TESTOSTERONE BINDING TO SEX HORMONE BINDING GLOBULIN IN VITRO AND IN VIVO

1986 ◽  
Vol 24 (5) ◽  
pp. 555-563 ◽  
Author(s):  
M. DOWSETT ◽  
K. L. FORBES ◽  
GILLIAN L. ROSE ◽  
JOANNE E. MUDGE ◽  
S. L. JEFFCOATE
Keyword(s):  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding

Internalization of Sex Hormone-Binding Globulin into Neurons and Brain Cells in vitro and in vivo

2007 ◽  
Vol 86 (2) ◽  
pp. 84-93 ◽  
Author(s):  
J.D. Caldwell ◽  
R.A. Shapiro ◽  
G.F. Jirikowski ◽  
F. Suleman
Keyword(s):  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Brain Cells ◽  
Hormone Binding

Influence of sex hormone binding globulin and serum albumin on the conversion of androstenedione to testosterone by human erythrocytes

1981 ◽  
Vol 96 (1) ◽  
pp. 136-140 ◽  
Author(s):  
M. Egloff ◽  
N. Savouré ◽  
J. Tardivel-Lacombe ◽  
C. Massart ◽  
M. Nicol ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Binding Sites ◽  
Sex Hormone ◽  
Human Erythrocytes ◽  
Sex Hormone Binding Globulin ◽  
Total Plasma ◽  
Hormone Binding ◽  
High Binding Affinity

Abstract. The influence of human serum albumin and sex hormone binding globulin (SHBG) on the enzymic conversion of androstenedione to testosterone in human erythrocytes was investigated in vitro. Total plasma and albumin delayed the conversion rate of androstenedione, while SHBG increased it markedly. The effect of SHBG was largely abolished by heating to 60°C for 1 h and by saturating its binding sites by DHT. The effect of both proteins was found to be related to their concentration. It appears that the binding sites of albumin provide a mechanism for retarding androstenedione uptake by the erythrocytes and that the high binding affinity of SHBG for testosterone facilitates the diffusion of this steroid out of the cell and thus, displaces the chemical equilibrium within the cell.

scholarly journals Protective Effect of Sex Hormone-Binding Globulin against Metabolic Syndrome: In Vitro Evidence Showing Anti-Inflammatory and Lipolytic Effects on Adipocytes and Macrophages

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Hiroki Yamazaki ◽  
Akifumi Kushiyama ◽  
Hideyuki Sakoda ◽  
Midori Fujishiro ◽  
Takeshi Yamamotoya ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Protective Effect ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Lipid Degradation ◽  
Protein Levels ◽  
Anti Inflammatory ◽  
20 Nm

Sex hormone-binding globulin (SHBG) is a serum protein released mainly by the liver, and a low serum level correlates with a risk for metabolic syndrome including diabetes, obesity, and cardiovascular events. However, the underlying molecular mechanism(s) linking SHBG and metabolic syndrome remains unknown. In this study, using adipocytes and macrophages, we focused on the in vitro effects of SHBG on inflammation as well as lipid metabolism. Incubation with 20 nM SHBG markedly suppressed lipopolysaccharide- (LPS-) induced inflammatory cytokines, such as MCP-1, TNFα, and IL-6 in adipocytes and macrophages, along with phosphorylations of JNK and ERK. Anti-inflammatory effects were also observed in 3T3-L1 adipocytes cocultured with LPS-stimulated macrophages. In addition, SHBG treatment for 18 hrs or longer significantly induced the lipid degradation of differentiated 3T3-L1 cells, with alterations in its corresponding gene and protein levels. Notably, these effects of SHBG were not altered by coaddition of large amounts of testosterone or estradiol. In conclusion, SHBG suppresses inflammation and lipid accumulation in macrophages and adipocytes, which might be among the mechanisms underlying the protective effect of SHBG, that is, its actions which reduce the incidence of metabolic syndrome.

scholarly journals Sex hormone-binding globulin regulation of androgen bioactivity in vivo: validation of the free hormone hypothesis

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Michaël R. Laurent ◽  
Geoffrey L. Hammond ◽  
Marco Blokland ◽  
Ferran Jardí ◽  
Leen Antonio ◽  
...  
Keyword(s):  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
In Vivo Validation
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