Antibodies against striated muscle, connective tissue and nuclear antigens in patients with thyroid-associated ophthalmopathy: Should Graves’ disease be considered a collagen disorder?

1997 ◽  
Vol 20 (10) ◽  
pp. 585-591 ◽  
Author(s):  
J. I. Kiljanski ◽  
K. Peele ◽  
I. Stachura ◽  
J. Pickeral ◽  
C. Stolarski ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Striated Muscle ◽  
Graves Disease ◽  
Nuclear Antigens ◽  
Thyroid Associated Ophthalmopathy

scholarly journals Detection of Specific Antinuclear Reactivities in Patients with Negative Anti-nuclear Antibody Immunofluorescence Screening Tests

2002 ◽  
Vol 48 (12) ◽  
pp. 2171-2176 ◽  
Author(s):  
Ilse EA Hoffman ◽  
Isabelle Peene ◽  
Eric M Veys ◽  
Filip De Keyser
Keyword(s):  
Connective Tissue ◽  
Confidence Interval ◽  
Connective Tissue Disease ◽  
Indirect Immunofluorescence ◽  
Connective Tissue Diseases ◽  
Clinical Suspicion ◽  
Screening Tests ◽  
Strongly Correlated ◽  
Nuclear Antigens

Abstract Background: For detection of anti-nuclear antibodies (ANAs) and antibodies to extractable nuclear antigens (ENAs), samples frequently are screened with indirect immunofluorescence (IIF); further determination of anti-ENA antibodies is performed only when the result is positive. However, because anti-ENA reactivities are found in samples with low fluorescence intensities, we determined anti-ENA antibodies in samples with negative IIF and thus calculated the sensitivity of IIF for specific ANAs. Methods: We collected 494 samples consecutively referred by rheumatologists for routine ANA testing. IIF on HEp-2 and HEp-2000 (HEp-2 cells transfected with Ro60 cDNA) and line immunoassay (LIA) for the detection of specific ANAs were performed on all samples. Results: Fluorescence intensities and patterns on HEp-2 were strongly correlated with those on HEp-2000 [Spearman ρ = 0.852 (P <0.001) and 0.838 (P <0.001), respectively]. Sixty-eight of 494 samples were positive on LIA, of which only 72% (confidence interval, 68–76%) were detected with HEp-2 and 75% (confidence interval, 70–78%) with HEp-2000. Of 291 samples negative on both substrates, 12 were positive on LIA. Connective tissue diseases were diagnosed in four of these patients and suspected in at least three others. Conclusion: The HEp-2 and HEp-2000 substrates perform comparably for fluorescence intensities and patterns and for detecting specific ANAs, but some patients with negative IIF show reactivity on LIA. We recommend testing for fine reactivities, regardless of the IIF result, when the clinical suspicion for rheumatic connective tissue disease is high.

Polymorphisms of IRAK1 Gene on X Chromosome Is Associated with Hashimoto Thyroiditis in Korean Children

Endocrinology ◽  
2020 ◽  
Vol 161 (8) ◽  
Author(s):  
Hye-Ri Shin ◽  
Won Kyoung Cho ◽  
In-Cheol Baek ◽  
Na Yeong Lee ◽  
Yoon Ji Lee ◽  
...  
Keyword(s):  
X Chromosome ◽  
Hashimoto Thyroiditis ◽  
Categorical Variables ◽  
Graves Disease ◽  
Strong Linkage Disequilibrium ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Korean Children ◽  
Autoimmune Thyroid ◽  
Thyroid Associated Ophthalmopathy

Abstract Autoimmune thyroid disease (AITD) is predominant in females and has been focused on the sexual diploid in immune response. The IL-1 receptor-associated kinase 1 (IRAK1) gene on the X chromosome was recently suggested as strong autoimmune disease-susceptible loci, second to the major histocompatibility complex region. We investigated the frequency of IRAK1 single-nucleotide polymorphisms (SNPs) in children with AITD. In this study, we observed that SNPs of IRAK1 including rs3027898, rs1059703, and rs1059702 in 115 Korean AITD pediatric patients (Graves’ disease = 74 [females = 52/males = 22]; Hashimoto disease [HD] = 41 [females = 38/males = 3]; thyroid-associated ophthalmopathy [TAO] = 40 (females = 27/males = 13); without TAO = 75 (females = 63/males = 12); total males = 25, total females = 90; mean age = 11.9 years) and 204 healthy Korean individuals (males = 104/females = 100). The data from cases and controls were analyzed from separate sex-stratified or all combined by χ 2 test for categorical variables and Student t test for numerical variables. Our study revealed that SNPs of IRAK1-associated HD and without TAO but Graves’ disease and TAO were not found significant. When cases and controls were analyzed by separate sex, we found that rs3027898 AA, rs1059703 AA, and rs1059702 GG showed disease susceptibility in female AITD, HD, and without TAO. Also, all rs3027898, rs1059703, and rs1059702 were found to be in strong linkage disequilibrium (D′ = 0.96-0.98, r2 = 0.83–0.97). The haplotype of 3 SNPs was higher in AITD than in controls (CGA, r2 = 5.42, P = 0.019). Our results suggest that IRAK1 polymorphisms may contribute to the pathogenesis of HD, AITD, and without thyroid-associated ophthalmopathy for females.

Staining of antinuclear antibodies and antibodies against removable nuclear antigens in connective tissue diseases

2020 ◽  
Vol 48 (1) ◽  
pp. 18-25
Author(s):  
José Enrique Oliva Menacho ◽  
Jorge Luis Arroyo-Acevedo ◽  
José Arturo Oliva-Candela ◽  
Marco Antonio García-Hjarles ◽  
Lester Domínguez-Huarcaya
Keyword(s):  
Connective Tissue ◽  
Antinuclear Antibodies ◽  
Connective Tissue Diseases ◽  
Nuclear Antigens

scholarly journals Immunocytochemical localization of fibronectin in limb tissues of the adult newt, Notophthalmus viridescens.

1981 ◽  
Vol 29 (8) ◽  
pp. 937-945 ◽  
Author(s):  
L A Repesh ◽  
L T Furcht ◽  
D Smith
Keyword(s):  
Connective Tissue ◽  
Striated Muscle ◽  
Basement Membranes ◽  
Similar Distribution ◽  
Immunocytochemical Localization ◽  
Tissue Scaffold ◽  
Cell Matrix ◽  
Tissue Organization ◽  
Adult Newt ◽  
Cell Matrix Interactions

This study describes the immunocytochemical localization of fibronectin, a defined connective tissue and plasma glycoprotein, and its relationship to collagen and reticulin in adult newt limb tissues. We have also isolated the plasma form of fibronectin in a related species, the adult mudpuppy. The insoluble form of fibronectin was detected with immunoperoxidase stain in basement membranes and loose connective tissue. The endoneurium and perineurium of nerve bundles and the connective tissue elements of striated muscle stained heavily for fibronectin. The dermis and blood vessel walls also reached positively with the immunoperoxidase stain. A similar distribution was observed for reticulin with conventional histologic techniques with the exception of the dermis where only trace amounts of the protein were observed. Fibronectin and collagen were codistributed in the tissues studies. Fibronectin appeared to be intercalated among larger collagenous fibers. Collagen and fibronectin form an extracellular connective tissue scaffold that abuts against many types of adherent cells in different tissues. This supports the possible role of fibronectin in cell-matrix interactions and normal cell and tissue organization.

Studies on the Sm and RNP nuclear antigens of human autoimmune rheumatic and connective-tissue diseases

1981 ◽  
Vol 9 (1) ◽  
pp. 144-145
Author(s):  
SOHRAB DAHI ◽  
GEORGE NAXAKIS ◽  
CHRISTINE M. WALLIS ◽  
ALEXANDER J. MacGILLIVRAY
Keyword(s):  
Connective Tissue ◽  
Connective Tissue Diseases ◽  
Nuclear Antigens

Differentially expressed circular RNAs in orbital adipose/connective tissue from patients with thyroid-associated ophthalmopathy

2020 ◽  
Vol 196 ◽  
pp. 108036 ◽  
Author(s):  
Lianqun Wu ◽  
Rongmei Zhou ◽  
Jiale Diao ◽  
Xinxin Chen ◽  
Jiancheng Huang ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Differentially Expressed ◽  
Circular Rnas ◽  
Thyroid Associated Ophthalmopathy

Functional morphology of the membranous lobe within the preoral cavity of Thenus orientalis (Crustacea: Scyllaridae)

1994 ◽  
Vol 74 (4) ◽  
pp. 787-800
Author(s):  
D.J. Johnston
Keyword(s):  
Connective Tissue ◽  
Functional Morphology ◽  
Cell Nucleus ◽  
Striated Muscle ◽  
Peripheral Cell ◽  
Food Items ◽  
Morphology And Structure ◽  
Oral Surface ◽  
Spherical Cells

The membranous lobe of Thenus orientalis (Lund) is a broad fleshy protuberance arising midventrally from the sternal skeleton between the second maxillipeds. Its position, morphology and structure are described and its association with the metastome of other crustaceans discussed. Several roles during ingestion can be postulated. The lobe extends anteriorly towards the mandibles and forms the roof of the preoral cavity and posterior boundary of the mouth. It has a flattened strengthening rod extending the width of the aboral surface, which is covered by three types of anteriorly-directed annulate setae and densely arranged clusters of microscales and microspines. Histologically it is characterized by large amounts of longitudinal and circular striated muscle and tegumental glands beneath the oral surface. These glands secrete sulphated acidic mucopolysaccharides which lubricate food during ingestion. Spherical cells are dispersed throughout the connective tissue beneath the aboral surface. These are characterized by a granular homogeneous cytoplasm, a peripheral cell nucleus and few organelles. Their contents stain positively for protein and neutral mucopolysaccharides and their role is most likely storage of these compounds, although the significance of this is not known. During ingestion, food is passed over the lobe surface and its anterior lip retracts ventro-posteriorly, dilating the preoral cavity to allow quick and efficient swallowing of large food items.

scholarly journals Cytokines, Graves' Disease, and Thyroid-Associated Ophthalmopathy

Thyroid ◽  
2008 ◽  
Vol 18 (9) ◽  
pp. 953-958 ◽  
Author(s):  
Andrew G. Gianoukakis ◽  
Nicole Khadavi ◽  
Terry J. Smith
Keyword(s):  
Graves Disease ◽  
Thyroid Associated Ophthalmopathy

Alteration of the connective tissue network of striated muscle in copper deficient rats

1985 ◽  
Vol 17 (12) ◽  
pp. 1173-1183 ◽  
Author(s):  
T BORG ◽  
L KLEVAY ◽  
R GAY ◽  
R SIEGEL ◽  
M BERGIN
Keyword(s):  
Connective Tissue ◽  
Striated Muscle
Sign in / Sign up

Export Citation Format

Share Document