In Vitro Regeneration from Internodal Explants and Somaclonal Variation in Chickpea (Cicer arietinum L)

2001 ◽  
Vol 10 (2) ◽  
pp. 107-112 ◽  
Author(s):  
P. K. Roy ◽  
M. L. Lodha ◽  
S. L. Mehta
Keyword(s):  
Somaclonal Variation ◽  
Cicer Arietinum ◽  
In Vitro Regeneration ◽  
Cicer Arietinum L

scholarly journals In vitro regeneration and induction of multiple shooting in Cicer arietinum L. using cotyledonary nodal explants

2015 ◽  
Vol 14 (13) ◽  
pp. 1129-1138 ◽  
Author(s):  
Prema Sunil Sruthi ◽  
Philip Robinson J ◽  
S KarthickBalan S ◽  
Anandhaprabhakaran M ◽  
Balakrishnan V
Keyword(s):  
Cicer Arietinum ◽  
In Vitro Regeneration ◽  
Nodal Explants ◽  
Multiple Shooting ◽  
Cicer Arietinum L

In vitro regeneration of chickpea (Cicer arietinum L.): Stimulation of direct organogenesis and somatic embryogenesis by thidiazuron

1996 ◽  
Vol 19 (3) ◽  
pp. 233-240 ◽  
Author(s):  
B. N. S. Murthy ◽  
Jerrin Victor ◽  
Rana P. Singh ◽  
R. A. Fletcher ◽  
Praveen K. Saxena
Keyword(s):  
Somatic Embryogenesis ◽  
Cicer Arietinum ◽  
In Vitro Regeneration ◽  
Direct Organogenesis ◽  
Cicer Arietinum L ◽  
Stimulation Of

scholarly journals In vitro Regeneration and Over Expression of Pea DNA Helicase 45 (PDH45) Gene into the Local Cultivars of Chickpea (Cicer arietinum L.) through Agrobacteriummediated Genetic Transformation

2018 ◽  
Vol 28 (1) ◽  
pp. 125-140
Author(s):  
Nuram Mubina ◽  
MI Hoque ◽  
RH Sarker
Keyword(s):  
Genetic Transformation ◽  
Cicer Arietinum ◽  
Zygotic Embryo ◽  
In Vitro Regeneration ◽  
Dna Helicase ◽  
Salt Tolerant ◽  
Agrobacterium Strain ◽  
Cicer Arietinum L ◽  
Gus Histochemical Assay

In vitro regeneration studies compatible to Agrobacterium-mediated genetic transformation were carried out using two different types of zygotic embryo derived explants namely, decapitated embryo (DE) and decapitated embryo with single cotyledon disc (DEC) from three varieties of chickpea (Cicer arietinum L.) such as BARI chhola-4, -5 and -9 cultivated in Bangladesh. The best responses towards in vitro shoot regeneration was obtained from decapitated embryo with DEC on MS containing 0.5 mg/l BAP, 0.5 mg/l Kn and 0.2 mg/l NAA. Healthy and effective roots from the regenerated shoots were developed on MS supplemented with 0.2 mg/l IBA. Genetic transformation was carried out with Agrobacterium strain LBA4404 containing the binary plasmid pCAMBIA1301- PDH45 to integrate salt tolerant PDH45 gene in locally grown varieties of chickpea. The transformed plantlets were successfully established in soil following adequate hardening. Integration of salt tolerant PDH45 gene within the genomic DNA was confirmed through GUS histochemical assay and PCR analysis.Plant Tissue Cult. & Biotech. 28(1): 125-140, 2018 (June)

Über den Abbau von Flavonolen in pflanzlichen Zellsuspensionskulturen / Degradation of Flavonols in Plant Suspension Cultures

1972 ◽  
Vol 27 (8) ◽  
pp. 946-954 ◽  
Author(s):  
Wolfgang Hösel ◽  
Paul D. Shaw ◽  
Wolfgang Barz
Keyword(s):  
Salicylic Acid ◽  
Glycine Max ◽  
Cell Suspension ◽  
Cicer Arietinum ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Enzyme Preparations ◽  
Cicer Arietinum L

The flavonols kaempferol, quercetin and isorhamnetin were labelled with 14C by keeping seven day old Cicer arietinum L. plants in an atmosphere of 14CO2 for five days. The purified (U-14C) flavonols were applied to cell suspension cultures of Cicer arietinum L., Phaseolus aureus Roxb., Glycine max and Petroselinum hortense. Based on the rates of 14CO2 formation and distribution of radioactivity after fractionation of the cells, the flavonols were shown to be catabolized to a very high extent.All four cell suspension cultures possess the enzymatic activity transforming flavonols to the recently discovered 2,3-dihydroxyflavanones. Upon incubation of the flavonols datiscetin and kaempferol with enzyme preparations from Cicer arietinum L. cell suspension cultures, it was demonstrated that the enzymatically formed 2,3-dihydroxyflavanones are further transformed in an enzyme catalyzed reaction. Salicylic acid was found as a degradation fragment of ring B of the 2,3,5,7,2′-pentahydroxyflavanone derived from datiscetin. Neither phloroglucinol nor phloroglucinol carboxylic acid were observed as metabolites of ring A. These in vitro findings were further substantiated by in vivo data because the flavonols kaempferol, quercetin and datiscetin when applied to cell suspension cultures of Cicer arietinum L. and Glycine max gave rise to para-hydroxybenzoic acid, protocatechuic acid and salicylic acid, respectively. It was thus concluded that flavonols are catabolized via 2,3-dihydroxyflavanones with the B-ring liberated as the respective benzoic acid. The data are discussed in connection with earlier findings on the catabolism of chalcones, cinnamic and benzoic acids.

scholarly journals Date palm micropropagation: Advances and applications

2017 ◽  
Vol 41 (4) ◽  
pp. 347-358 ◽  
Author(s):  
Jameel Mohammed Al-Khayri ◽  
Poornananda Madhava Naik
Keyword(s):  
Somaclonal Variation ◽  
Large Scale ◽  
Date Palm ◽  
In Vitro Regeneration ◽  
Phoenix Dactylifera ◽  
Climatic Conditions ◽  
Chemical Components ◽  
Palm Trees ◽  
Adult Trees

ABSTRACT Date palm (Phoenix dactylifera L.) is a fruit tree resilient to adverse climatic conditions predominating in hot arid regions of the Middle East and North Africa. The date fruit contains numerous chemical components that possess high nutritional and medicinal values. Traditional propagation by offshoots is inefficient to satisfy current demands for date palm trees. Alternatively, micropropagation provides an efficient means for large-scale propagation of date palm cultivars. Both somatic embryogenesis and organogenesis, either directly or indirectly though the callus phase, have been demonstrated in date palm in vitro regeneration. Culture initiation commonly utilizes shoot-tip explants isolated from young offshoots. Recently, the immature inflorescences of adult trees were utilized as an alternative nondestructive source of explants. In addition to the nature of the explant used, successful plant regeneration depends on the cultivar, composition of the culture medium and physical status. Challenges of date palm micropropagation include long in vitro cycle, latent contamination, browning, somaclonal variation as well as ex vitro acclimatization and transplanting. A remarkable amount of research investigating these factors has led to optimized protocols for the micropropagation of numerous commercially important cultivars. This has encouraged the development of several international commercial tissue culture laboratories. Molecular characterization provides an assurance of genetic conformity of regenerated plantlets, a key feature for commercial production. This article describes date palm micropropagation protocols and also discusses recent achievements with respect to somaclonal variation, molecular markers, cryopreservation and future prospects.

scholarly journals Caracterização e hidrólise in vitro da globulina principal de grão-de-bico (Cicer arietinum L.), var. IAC-Marrocos

2004 ◽  
Vol 24 (1) ◽  
pp. 139-145 ◽  
Author(s):  
Valdir A. Neves ◽  
Maraiza A. da Silva ◽  
Euclides J. Lourenço
Keyword(s):  
Cicer Arietinum ◽  
Cicer Arietinum L
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