In vitro regeneration of chickpea (Cicer arietinum L.): Stimulation of direct organogenesis and somatic embryogenesis by thidiazuron

1996 ◽  
Vol 19 (3) ◽  
pp. 233-240 ◽  
Author(s):  
B. N. S. Murthy ◽  
Jerrin Victor ◽  
Rana P. Singh ◽  
R. A. Fletcher ◽  
Praveen K. Saxena
Keyword(s):  
Somatic Embryogenesis ◽  
Cicer Arietinum ◽  
In Vitro Regeneration ◽  
Direct Organogenesis ◽  
Cicer Arietinum L ◽  
Stimulation Of

scholarly journals In vitro regeneration and induction of multiple shooting in Cicer arietinum L. using cotyledonary nodal explants

2015 ◽  
Vol 14 (13) ◽  
pp. 1129-1138 ◽  
Author(s):  
Prema Sunil Sruthi ◽  
Philip Robinson J ◽  
S KarthickBalan S ◽  
Anandhaprabhakaran M ◽  
Balakrishnan V
Keyword(s):  
Cicer Arietinum ◽  
In Vitro Regeneration ◽  
Nodal Explants ◽  
Multiple Shooting ◽  
Cicer Arietinum L

scholarly journals In vitro Regeneration and Over Expression of Pea DNA Helicase 45 (PDH45) Gene into the Local Cultivars of Chickpea (Cicer arietinum L.) through Agrobacteriummediated Genetic Transformation

2018 ◽  
Vol 28 (1) ◽  
pp. 125-140
Author(s):  
Nuram Mubina ◽  
MI Hoque ◽  
RH Sarker
Keyword(s):  
Genetic Transformation ◽  
Cicer Arietinum ◽  
Zygotic Embryo ◽  
In Vitro Regeneration ◽  
Dna Helicase ◽  
Salt Tolerant ◽  
Agrobacterium Strain ◽  
Cicer Arietinum L ◽  
Gus Histochemical Assay

In vitro regeneration studies compatible to Agrobacterium-mediated genetic transformation were carried out using two different types of zygotic embryo derived explants namely, decapitated embryo (DE) and decapitated embryo with single cotyledon disc (DEC) from three varieties of chickpea (Cicer arietinum L.) such as BARI chhola-4, -5 and -9 cultivated in Bangladesh. The best responses towards in vitro shoot regeneration was obtained from decapitated embryo with DEC on MS containing 0.5 mg/l BAP, 0.5 mg/l Kn and 0.2 mg/l NAA. Healthy and effective roots from the regenerated shoots were developed on MS supplemented with 0.2 mg/l IBA. Genetic transformation was carried out with Agrobacterium strain LBA4404 containing the binary plasmid pCAMBIA1301- PDH45 to integrate salt tolerant PDH45 gene in locally grown varieties of chickpea. The transformed plantlets were successfully established in soil following adequate hardening. Integration of salt tolerant PDH45 gene within the genomic DNA was confirmed through GUS histochemical assay and PCR analysis.Plant Tissue Cult. & Biotech. 28(1): 125-140, 2018 (June)

scholarly journals In vitro Regeneration of Dryland Kenyan Maize Genotypes Through Somatic Embryogenesis

2006 ◽  
Vol 2 (2) ◽  
pp. 146-151 ◽  
Author(s):  
R.O. Oduor ◽  
E.N.M. Njagi ◽  
S. Ndung` u ◽  
J.S. Machuka
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Regeneration ◽  
Maize Genotypes

In vitro Regeneration of Irvingia gabonensis by Somatic Embryogenesis

2008 ◽  
Vol 11 (5) ◽  
pp. 726-732 ◽  
Author(s):  
Fotso . ◽  
Oumar . ◽  
Niemenak Nicolas ◽  
Donfagsiteli Tchinda Ne ◽  
Omokolo Ndoumou De
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Regeneration ◽  
Irvingia Gabonensis
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