Over-expression of Osmotin Induces Proline Accumulation and Confers Tolerance to Osmotic Stress in Transgenic Tobacco

2001 ◽  
Vol 10 (1) ◽  
pp. 31-37 ◽  
Author(s):  
S. Barthakur ◽  
V. Babu ◽  
K. C. Bansa
Keyword(s):  
Osmotic Stress ◽  
Transgenic Tobacco ◽  
Proline Accumulation ◽  
Over Expression

Seed germination and seedling vigour of transgenic tobacco (Nicotiana tabacum L.) with increased proline accumulation under osmotic stress

2018 ◽  
Vol 94 (2) ◽  
pp. 220-228 ◽  
Author(s):  
Fabiana de Lima Abrantes ◽  
Alessandra Ferreira Ribas ◽  
Luiz Gonzaga Esteves Vieira ◽  
Nelson Barbosa Machado-Neto ◽  
Ceci Castilho Custódio
Keyword(s):  
Nicotiana Tabacum ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Transgenic Tobacco ◽  
Proline Accumulation ◽  
Seedling Vigour ◽  
Nicotiana Tabacum L

scholarly journals Rootstocks Overexpressing StNPR1 and StDREB1 Improve Osmotic Stress Tolerance of Wild-Type Scion in Transgrafted Tobacco Plants

2021 ◽  
Vol 22 (16) ◽  
pp. 8398
Author(s):  
Yasmine S. Hezema ◽  
Mukund R. Shukla ◽  
Alok Goel ◽  
Murali M. Ayyanath ◽  
Sherif M. Sherif ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Transgenic Tobacco ◽  
Physiological Status ◽  
Wild Type ◽  
Long Distance ◽  
Graft Union ◽  
Tobacco Plants ◽  
Osmotic Stress Tolerance ◽  
And Function ◽  
Gene Expression Levels

In grafted plants, the movement of long-distance signals from rootstocks can modulate the development and function of the scion. To understand the mechanisms by which tolerant rootstocks improve scion responses to osmotic stress (OS) conditions, mRNA transport of osmotic responsive genes (ORGs) was evaluated in a tomato/potato heterograft system. In this system, Solanum tuberosum was used as a rootstock and Solanum lycopersicum as a scion. We detected changes in the gene expression levels of 13 out of the 21 ORGs tested in the osmotically stressed plants; of these, only NPR1 transcripts were transported across the graft union under both normal and OS conditions. Importantly, OS increased the abundance of StNPR1 transcripts in the tomato scion. To examine mRNA mobility in transgrafted plants, StNPR1 and StDREB1 genes representing the mobile and non-mobile transcripts, respectively, were overexpressed in tobacco (Nicotiana tabacum). The evaluation of transgenic tobacco plants indicated that overexpression of these genes enhanced the growth and improved the physiological status of transgenic plants growing under OS conditions induced by NaCl, mannitol and polyethylene glycol (PEG). We also found that transgenic tobacco rootstocks increased the OS tolerance of the WT-scion. Indeed, WT scions on transgenic rootstocks had higher ORGs transcript levels than their counterparts on non-transgenic rootstocks. However, neither StNPR1 nor StDREB1 transcripts were transported from the transgenic rootstock to the wild-type (WT) tobacco scion, suggesting that other long-distance signals downstream these transgenes could have moved across the graft union leading to OS tolerance. Overall, our results signify the importance of StNPR1 and StDREB1 as two anticipated candidates for the development of stress-resilient crops through transgrafting technology.

scholarly journals Over-expression of poplar NAC15 gene enhances wood formation in transgenic tobacco

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Wenjing Yao ◽  
Dawei Zhang ◽  
Boru Zhou ◽  
Jianping Wang ◽  
Renhua Li ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Wood Formation ◽  
Potential Candidate ◽  
Rna Seq ◽  
Populus Simonii ◽  
Over Expression ◽  
Potential Candidate Gene ◽  
Relative Expression Level ◽  
Transgenic Lines ◽  
Highly Expressed Genes

Abstract Background NAC (NAM/ATAF/CUC) is one of the largest plant-specific transcription factor (TF) families known to play significant roles in wood formation. Acting as master gene regulators, a few NAC genes can activate secondary wall biosynthesis during wood formation in woody plants. Results In the present study, firstly, we screened 110 differentially expressed NAC genes in the leaves, stems, and roots of di-haploid Populus simonii×P. nigra by RNA-Seq. Then we identified a nucleus-targeted gene, NAC15 gene, which was one of the highly expressed genes in the stem among 110 NAC family members. Thirdly, we conducted expression pattern analysis of NAC15 gene, and observed NAC15 gene was most highly expressed in the xylem by RT-qPCR. Moreover, we transferred NAC15 gene into tobacco and obtained 12 transgenic lines overexpressing NAC15 gene (TLs). And the relative higher content of hemicellulose, cellulose and lignin was observed in the TLs compared to the control lines containing empty vector (CLs). It also showed darker staining in the culms of the TLs with phloroglucinol staining, compared to the CLs. Furthermore, the relative expression level of a few lignin- and cellulose-related genes was significantly higher in the TLs than that in the CLs. Conclusions The overall results indicated that NAC15 gene is highly expressed in the xylem of poplar and may be a potential candidate gene playing an important role in wood formation in transgenic tobacco.

Over-expression of PIP2;5 aquaporin alleviates gas exchange and growth inhibition in poplars exposed to mild osmotic stress with polyethylene glycol

2017 ◽  
Vol 39 (8) ◽  
Author(s):  
Kapilan Ranganathan ◽  
Janice E. K. Cooke ◽  
Walid El Kayal ◽  
Maria A. Equiza ◽  
Maryamsadat Vaziriyeganeh ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Gas Exchange ◽  
Osmotic Stress ◽  
Growth Inhibition ◽  
Over Expression
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