scholarly journals Light-induced expression of ipt from Agrobacterium tumefaciens results in cytokinin accumulation and osmotic stress symptoms in transgenic tobacco

1995 ◽  
Vol 28 (5) ◽  
pp. 965-965 ◽  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Osmotic Stress ◽  
Transgenic Tobacco ◽  
Induced Expression ◽  
Stress Symptoms

scholarly journals Rootstocks Overexpressing StNPR1 and StDREB1 Improve Osmotic Stress Tolerance of Wild-Type Scion in Transgrafted Tobacco Plants

2021 ◽  
Vol 22 (16) ◽  
pp. 8398
Author(s):  
Yasmine S. Hezema ◽  
Mukund R. Shukla ◽  
Alok Goel ◽  
Murali M. Ayyanath ◽  
Sherif M. Sherif ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Transgenic Tobacco ◽  
Physiological Status ◽  
Wild Type ◽  
Long Distance ◽  
Graft Union ◽  
Tobacco Plants ◽  
Osmotic Stress Tolerance ◽  
And Function ◽  
Gene Expression Levels

In grafted plants, the movement of long-distance signals from rootstocks can modulate the development and function of the scion. To understand the mechanisms by which tolerant rootstocks improve scion responses to osmotic stress (OS) conditions, mRNA transport of osmotic responsive genes (ORGs) was evaluated in a tomato/potato heterograft system. In this system, Solanum tuberosum was used as a rootstock and Solanum lycopersicum as a scion. We detected changes in the gene expression levels of 13 out of the 21 ORGs tested in the osmotically stressed plants; of these, only NPR1 transcripts were transported across the graft union under both normal and OS conditions. Importantly, OS increased the abundance of StNPR1 transcripts in the tomato scion. To examine mRNA mobility in transgrafted plants, StNPR1 and StDREB1 genes representing the mobile and non-mobile transcripts, respectively, were overexpressed in tobacco (Nicotiana tabacum). The evaluation of transgenic tobacco plants indicated that overexpression of these genes enhanced the growth and improved the physiological status of transgenic plants growing under OS conditions induced by NaCl, mannitol and polyethylene glycol (PEG). We also found that transgenic tobacco rootstocks increased the OS tolerance of the WT-scion. Indeed, WT scions on transgenic rootstocks had higher ORGs transcript levels than their counterparts on non-transgenic rootstocks. However, neither StNPR1 nor StDREB1 transcripts were transported from the transgenic rootstock to the wild-type (WT) tobacco scion, suggesting that other long-distance signals downstream these transgenes could have moved across the graft union leading to OS tolerance. Overall, our results signify the importance of StNPR1 and StDREB1 as two anticipated candidates for the development of stress-resilient crops through transgrafting technology.

Precursor processing by SBT3.8 and phytosulfokine signaling contribute to drought stress tolerance in Arabidopsis

2021 ◽  
Author(s):  
Nils Stührwohldt ◽  
Eric Bühler ◽  
Margret Sauter ◽  
Andreas Schaller
Keyword(s):  
Drought Stress ◽  
Osmotic Stress ◽  
Stress Tolerance ◽  
Serine Proteases ◽  
Loss Of Function ◽  
Lateral Root Development ◽  
Osmotic Stress Tolerance ◽  
Stress Symptoms ◽  
C Terminus ◽  
Peptide Precursor

Abstract Increasing drought stress poses a severe threat to agricultural productivity. Plants, however, evolved numerous mechanisms to cope with such environmental stress. Here we report that the stress-induced production of a peptide signal contributes to stress tolerance. The expression of phytosulfokine (PSK) peptide precursor genes, and transcripts of three subtilisin-like serine proteases, SBT1.4, SBT3.7 and SBT3.8 were found to be up-regulated in response to osmotic stress. Stress symptoms were enhanced in sbt3.8 loss-of-function mutants and could be alleviated by PSK treatment. Osmotic stress tolerance was improved in plants overexpressing the precursor of PSK1 (proPSK1) or SBT3.8 resulting in higher fresh weight and improved lateral root development in the transgenic compared to wild-type plants. We further showed that SBT3.8 is involved in the biogenesis of the bioactive PSK peptide. ProPSK1 was cleaved by SBT3.8 at the C-terminus of the PSK pentapeptide. Processing by SBT3.8 depended on the aspartic acid residue directly following the cleavage site. ProPSK1 processing was impaired in the sbt3.8 mutant. The data suggest that increased expression in response to osmotic stress followed by the post-translational processing of proPSK1 by SBT3.8 leads to the production of PSK as a peptide signal for stress mitigation.

scholarly journals Induced Expression of Sarcotoxin IA Enhanced Host Resistance Against Both Bacterial and Fungal Pathogens in Transgenic Tobacco

2000 ◽  
Vol 13 (8) ◽  
pp. 860-868 ◽  
Author(s):  
Ichiro Mitsuhara ◽  
Hiroki Matsufuru ◽  
Masahiro Ohshima ◽  
Hisatoshi Kaku ◽  
Yuki Nakajima ◽  
...  
Keyword(s):  
Fungal Infection ◽  
Transgenic Tobacco ◽  
Pseudomonas Syringae ◽  
Host Resistance ◽  
Fungal Pathogens ◽  
Erwinia Carotovora ◽  
Induced Expression ◽  
Sarcotoxin Ia ◽  
Resistance To Infection

We demonstrate here that induced expression of sarcotoxin IA, a bactericidal peptide from Sarcophaga peregrina, enhanced the resistance of transgenic tobacco plants to both bacterial and fungal pathogens. The peptide was produced with a modified PR1a promoter, which is further activated by salicylic acid treatment and necrotic lesion formation by pathogen infection. Host resistance to infection of bacteria Erwinia carotovora subsp. carotovora and Pseudomonas syringae pv. tabaci was shown to be dependent on the amounts of sarcotoxin IA expressed. Since we found antifungal activity of the peptide in vitro, transgenic seedlings were also inoculated with fungal pathogens Rhizoctonia solani and Pythium aphanidermatum. Transgenic plants expressing higher levels of sarcotoxin were able to withstand fungal infection and remained healthy even after 4 weeks, while control plants were dead by fungal infection after 2 weeks.

Pathogen-induced expression of a cecropin A-melittin antimicrobial peptide gene confers antifungal resistance in transgenic tobacco

2005 ◽  
Vol 56 (416) ◽  
pp. 1685-1695 ◽  
Author(s):  
Dmytro P. Yevtushenko ◽  
Rafael Romero ◽  
Benjamin S. Forward ◽  
Robert E. Hancock ◽  
William W. Kay ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Transgenic Tobacco ◽  
Antifungal Resistance ◽  
Induced Expression ◽  
Cecropin A ◽  
Peptide Gene

Seed germination and seedling vigour of transgenic tobacco (Nicotiana tabacum L.) with increased proline accumulation under osmotic stress

2018 ◽  
Vol 94 (2) ◽  
pp. 220-228 ◽  
Author(s):  
Fabiana de Lima Abrantes ◽  
Alessandra Ferreira Ribas ◽  
Luiz Gonzaga Esteves Vieira ◽  
Nelson Barbosa Machado-Neto ◽  
Ceci Castilho Custódio
Keyword(s):  
Nicotiana Tabacum ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Transgenic Tobacco ◽  
Proline Accumulation ◽  
Seedling Vigour ◽  
Nicotiana Tabacum L
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