Demonstration of stereoselective disposition of warfarin enantiomers by whole-body autoradiography

1990 ◽  
Vol 15 (2) ◽  
pp. 103-107 ◽  
Author(s):  
I. Szinai ◽  
M. Simonyi ◽  
I. Fitos ◽  
Zs. Tegyey ◽  
A. Magyar
Keyword(s):  
Whole Body ◽  
Whole Body Autoradiography ◽  
Warfarin Enantiomers ◽  
Stereoselective Disposition

scholarly journals WHOLE-BODY AUTORADIOGRAPHY OF DISTRIBUTION OF [1-14C]-2-DEOXYGLUCOSE IN MICE

1980 ◽  
Vol 13 (2) ◽  
pp. 202-210 ◽  
Author(s):  
MASAHITO WATANABE ◽  
TAKASHI KIHARA ◽  
MASAHISA SHIMADA ◽  
KIYOHISA KURIMOTO
Keyword(s):  
Whole Body ◽  
Whole Body Autoradiography

Whole body autoradiography and microautoradiography in eels after intra-arterial administration of 125I-labeled eel ANP

1996 ◽  
Vol 271 (4) ◽  
pp. R926-R935 ◽  
Author(s):  
H. Sakaguchi ◽  
H. Suzuki ◽  
H. Hagiwara ◽  
H. Kaiya ◽  
Y. Takei ◽  
...  
Keyword(s):  
Binding Sites ◽  
Head Kidney ◽  
Dorsal Aorta ◽  
Whole Body ◽  
Whole Body Autoradiography ◽  
Target Organs ◽  
Secondary Lamellae ◽  
Ventral Aorta ◽  
Interrenal Cells ◽  
Kidney Liver

125I-labeled eel atrial natriuretic peptide (ANP) was administered into the ventral or dorsal aorta of freshwater (FW) and seawater (SW) eels, Anguilla japonica, and the major target organs were explored by whole body autoradiography. Localization of the ANP binding in the target organs was also examined at tissue and cell levels by microautoradiography using tissue sections. Whole body autoradiography revealed that the specific label was accumulated predominantly in the gill, with lesser amounts in the atrium, kidney, liver, and urinary bladder. Autoradiographic grains were most dense in the secondary lamellae of the gill, particularly on the side of the efferent filamental artery. Other binding sites in target tissues were the glomerulus of the kidney, epicardium and endocardium of the atrium, bile duct/blood vessels of the liver, and interrenal cells of the head kidney. There was no difference in the distribution and density of grains between injections into the ventral aorta and dorsal aorta, although, in the former, injected 125I-labeled eel ANP passes through the gill before reaching peripheral target tissues. There was a tendency for downregulation of ANP binding sites in SW eels, especially in the gill. These results show that specific ANP binding sites are present in organs that are implicated in osmoregulation and cardiovascular regulation in eels and further suggest that the number of ANP binding sites varies according to changes in the environmental salinity.

Whole Body Autoradiography

2004 ◽  
Author(s):  
Kenneth Brouwer ◽  
Lee Crossman ◽  
Jeanne Jarrett
Keyword(s):  
Whole Body ◽  
Whole Body Autoradiography

Distribution of 14C-labelled Atrazine, Methoxychlor, Glyphosate, and Bisphenol-A in Goldfish Studied by Whole-Body Autoradiography (WBARG)

2008 ◽  
Vol 43 (4) ◽  
pp. 265-274 ◽  
Author(s):  
Claude Rouleau ◽  
Jagmohan Kohli
Keyword(s):  
Bisphenol A ◽  
Carassius Auratus ◽  
Environmental Risks ◽  
Whole Body ◽  
Intestinal Lumen ◽  
Whole Body Autoradiography ◽  
Goldfish Carassius Auratus ◽  
Uveal Tract ◽  
Target Organs ◽  
Freeze Dried

Abstract Nonpersistent contaminants represent thousands of chemicals used as pesticides, pharmaceuticals, personal care products, additives, etc. Because of this diversity, the assessment of the environmental risks they may pose for the environment represents a formidable task. Identification of target organs is key information needed to orient further research on newlyinvestigated organic xenobiotics. We used whole-body autoradiography to visualize the distribution of 14C-labelled atrazine, methoxychlor, glyphosate, and bisphenol-A in goldfish (Carassius auratus) and identify target organs. Fish were exposed for 2 days (glyphosate and bisphenol-A) and 7 days (atrazine and methoxychlor) to the radiolabelled compounds at a concentration of 15 nM. They were then frozen, embedded in carboxymethylcellulose gel, 20-μm-thick cryosections were collected, freeze-dried, and exposed to phosphor screens to visualize the tissue distribution of radioactivity. Goldfish did not accumulate glyphosate. The three other compounds were accumulated, mostly in the gall bladder. Nevertheless, unforeseen accumulation sites were observed; atrazine accumulated in the uveal tract of the eye, high levels of radioactivity were found in the cerebrospinal fluid of goldfish exposed to methoxychlor, and an important accumulation of bisphenol-A was seen in urine, oral mucosa, esophagus, and intestinal lumen. The potential toxicological consequences of the accumulation of these chemicals at very specific locations within the fish body are discussed and further research suggested.

scholarly journals Quantitative method for whole body autoradiography and application to tissue distribution study of FO-1561 in rats.

1988 ◽  
Vol 3 (6) ◽  
pp. 747-760
Author(s):  
Eiichi NAKAJIMA ◽  
Yoshie YASUKAWA ◽  
Hayao SHINOZAKI ◽  
Yukio MATSUBARA ◽  
Shigeto FUJISHITA ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Quantitative Method ◽  
Whole Body ◽  
Distribution Study ◽  
Whole Body Autoradiography ◽  
Tissue Distribution Study
Sign in / Sign up

Export Citation Format

Share Document