Esterase electrophoretic polymorphism ofBacillus thuringiensis andBacillus cereus reference strains

2007 ◽  
Vol 57 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Ameur Cherif ◽  
Besma Ettoumi ◽  
Afef Najjari ◽  
Noura Raddadi ◽  
Abdellatif Boudabous
Keyword(s):  
Ofbacillus Thuringiensis ◽  
Electrophoretic Polymorphism ◽  
Reference Strains

GC-MS, HPLC, TLC of Dorycnium herbaceum Tincture Species and Synergistic / Antagonist Effect Testing in Combination with Antibiotics

2017 ◽  
Vol 68 (2) ◽  
pp. 228-231 ◽  
Author(s):  
Renata Maria Varut ◽  
Luciana Teodora Rotaru
Keyword(s):  
Chemical Composition ◽  
Clavulanic Acid ◽  
Carboxylic Acids ◽  
Therapeutic Effect ◽  
Antagonistic Effect ◽  
Hydroalcoholic Extract ◽  
Low Concentrations ◽  
Amoxicillin Clavulanic Acid ◽  
Reference Strains ◽  
Antagonist Effect

The study objectives were to determine the chemical composition and the synergistic / antagonistic effect of the association between hydroalcoholic extract from the Dorycnii pentaphylli herba (DPH) and the antibiotics of choice, on five reference strains. The tincture contains flavonoids and polyphenol carboxylic acids in low concentrations. DPH has an antagonistic effect on three of the drugs tested (amoxicillin + clavulanic acid, levofloxacin, amikacin), the therapeutic effect being completely canceled and has no significant effect on two of them (ceftazidime, cefotaxime).

scholarly journals Antibacterial Activity of Fluorobenzoylthiosemicarbazides and Their Cyclic Analogues with 1,2,4-Triazole Scaffold

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 170
Author(s):  
Urszula Kosikowska ◽  
Monika Wujec ◽  
Nazar Trotsko ◽  
Wojciech Płonka ◽  
Piotr Paneth ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Resistant Bacteria ◽  
Binding Affinities ◽  
Substitution Pattern ◽  
Minimal Inhibitory Concentrations ◽  
Gram Positive Bacteria ◽  
Structure Activity ◽  
Drug Resistant Bacteria ◽  
Reference Strains

The development of drug-resistant bacteria is currently one of the major challenges in medicine. Therefore, the discovery of novel lead structures for the design of antibacterial drugs is urgently needed. In this structure–activity relationship study, a library of ortho-, meta-, and para-fluorobenzoylthiosemicarbazides, and their cyclic analogues with 1,2,4-triazole scaffold, was created and tested for antibacterial activity against Gram-positive bacteria strains. While all tested 1,2,4-triazoles were devoid of potent activity, the antibacterial response of the thiosemicarbazides was highly dependent on substitution pattern at the N4 aryl position. The optimum activity for these compounds was found for trifluoromethyl derivatives such as 15a, 15b, and 16b, which were active against both the reference strains panel, and pathogenic methicillin-sensitive and methicillin-resistant Staphylococcus aureus clinical isolates at minimal inhibitory concentrations (MICs) ranging from 7.82 to 31.25 μg/mL. Based on the binding affinities obtained from docking, the conclusion can be reached that fluorobenzoylthiosemicarbazides can be considered as potential allosteric d-alanyl-d-alanine ligase inhibitors.

scholarly journals Thiazole Analogues of the Marine Alkaloid Nortopsentin as Inhibitors of Bacterial Biofilm Formation

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 81
Author(s):  
Anna Carbone ◽  
Stella Cascioferro ◽  
Barbara Parrino ◽  
Daniela Carbone ◽  
Camilla Pecoraro ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Thiazole Derivatives ◽  
Gram Positive ◽  
Lead Compounds ◽  
New Class ◽  
Marked Selectivity ◽  
Global Threat ◽  
New Compounds ◽  
Reference Strains

Anti-virulence strategy is currently considered a promising approach to overcome the global threat of the antibiotic resistance. Among different bacterial virulence factors, the biofilm formation is recognized as one of the most relevant. Considering the high and growing percentage of multi-drug resistant infections that are biofilm-mediated, new therapeutic agents capable of counteracting the formation of biofilms are urgently required. In this scenario, a new series of 18 thiazole derivatives was efficiently synthesized and evaluated for its ability to inhibit biofilm formation against the Gram-positive bacterial reference strains Staphylococcus aureus ATCC 25923 and S. aureus ATCC 6538 and the Gram-negative strain Pseudomonas aeruginosa ATCC 15442. Most of the new compounds showed a marked selectivity against the Gram-positive strains. Remarkably, five compounds exhibited BIC50 values against S. aureus ATCC 25923 ranging from 1.0 to 9.1 µM. The new compounds, affecting the biofilm formation without any interference on microbial growth, can be considered promising lead compounds for the development of a new class of anti-virulence agents.

scholarly journals Annotations from the NCTC on the list of type and reference strains of bacteria, Sneath and Skerman 1966

1967 ◽  
Vol 17 (2) ◽  
pp. 93-103 ◽  
Author(s):  
S. P. LAPAGE ◽  
L. R. HILL ◽  
J. MIDGLEY ◽  
J. E. SHELTON
Keyword(s):  
Reference Strains

scholarly journals Comparison of Ligation-Mediated PCR Methods in Differentiation ofMycobacterium tuberculosisStrains

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Anna Zaczek ◽  
Anna Brzostek ◽  
Arkadiusz Wojtasik ◽  
Anna Sajduda ◽  
Jaroslaw Dziadek
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Small Community ◽  
Discriminative Power ◽  
Epidemiological Analysis ◽  
Reference Strains

Fast and inexpensive identification of epidemiological links between limited number ofMycobacterium tuberculosisstrains is required to initially evaluate hospital outbreaks, laboratory crosscontaminations, and family or small community transmissions. The ligation-mediated PCR methods (LM-PCR) appear sufficiently discriminative and reproducible to be considered as a good candidate for such initial, epidemiological analysis. Here, we compared the discriminative power of the recently developed in our laboratory fast ligation amplification polymorphism (FLAP) method with fast ligation-mediated PCR (FLiP). Verification of the results was based on analyzing a set of reference strains and RFLP-IS6110typing. The HGDI value was very similar for both LM-PCR methods and RFLP-IS6110typing. However, only 52% of strains were correspondingly grouped by both FLiP and FLAP methods. Differentiation by FLAP method demonstrated a limited similarity to IS6110-RFLP (37,7%). As much as 78,7% of strains were grouped identically when differentiated by FLiP and IS6110-RFLP methods. The analysis differentiated 31, 35, and 36 groups when using FLAP, FLiP, and RFLP-IS6110methods, respectively.

scholarly journals Restriction Fragment Length Polymorphisms and Sequence Analysis: an Approach for Genotyping Infectious Pancreatic Necrosis Virus Reference Strains and Other Aquabirnaviruses Isolated from Northwestern Spain

2004 ◽  
Vol 70 (2) ◽  
pp. 1059-1067 ◽  
Author(s):  
J. M. Cutrín ◽  
J. L. Barja ◽  
B. L. Nicholson ◽  
I. Bandín ◽  
S. Blake ◽  
...  
Keyword(s):  
Pancreatic Necrosis ◽  
Restriction Analysis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Genotype I ◽  
Northwestern Spain ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus ◽  
Reference Strains ◽  
Restriction Fragment Length

ABSTRACT Reference strains of infectious pancreatic necrosis virus resembling the 10 recognized serotypes and local isolates of aquabirnaviruses isolated in northwestern Spain from reservoirs (mollusks) and from asymptomatic and carrier cultured fish were genotyped by restriction fragment length polymorphism (RFLP) and nucleic acid sequence analyses. The RFLP analysis yielded seven genogroups, each of which was clearly correlated with a serotype. Sequence analysis of the three open reading frames provided quite similar results in terms of genogrouping. Based on the results of this study and in order to unify the two types of assays, we propose placing aquabirnaviruses into six genogroups, four of which can be subdivided into two genotypes based on a two-step restriction analysis. The genotyping corresponds with serotyping as follows: genogroup I includes two genotypes corresponding to serotypes A9 (genotype I.1) and A1 (genotype I.2); genogroup II corresponds to serotype A3; genogroup III includes genotypes III.1 (serotype A2) and III.2 (serotype B1); genogroups IV and V include two genotypes, each corresponding to serotypes A5, A6, A7, and A8 (genotypes IV.1, IV.2, V.1, and V.2, respectively);and genogroup VI corresponds to serotype A4. As expected, most local isolates belonged to genotype III.1 and genogroup II. However, a few local isolates corresponded to the American types of genogroup I. Finally, based on the results of this study and due to its simplicity, the two-step restriction analysis assay is proposed as a method for typing new isolates of aquabirnaviruses, and the results correspond to the results of conventional serotyping.

scholarly journals Microarray-based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 88 ◽  
Author(s):  
Julien Gouré ◽  
Wendy A Findlay ◽  
Vincent Deslandes ◽  
Anne Bouevitch ◽  
Simon J Foote ◽  
...  
Keyword(s):  
Actinobacillus Pleuropneumoniae ◽  
Comparative Genomic ◽  
Genomic Profiling ◽  
Field Isolates ◽  
Reference Strains
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