scholarly journals Comparison of Ligation-Mediated PCR Methods in Differentiation ofMycobacterium tuberculosisStrains

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Anna Zaczek ◽  
Anna Brzostek ◽  
Arkadiusz Wojtasik ◽  
Anna Sajduda ◽  
Jaroslaw Dziadek
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Small Community ◽  
Discriminative Power ◽  
Epidemiological Analysis ◽  
Reference Strains

Fast and inexpensive identification of epidemiological links between limited number ofMycobacterium tuberculosisstrains is required to initially evaluate hospital outbreaks, laboratory crosscontaminations, and family or small community transmissions. The ligation-mediated PCR methods (LM-PCR) appear sufficiently discriminative and reproducible to be considered as a good candidate for such initial, epidemiological analysis. Here, we compared the discriminative power of the recently developed in our laboratory fast ligation amplification polymorphism (FLAP) method with fast ligation-mediated PCR (FLiP). Verification of the results was based on analyzing a set of reference strains and RFLP-IS6110typing. The HGDI value was very similar for both LM-PCR methods and RFLP-IS6110typing. However, only 52% of strains were correspondingly grouped by both FLiP and FLAP methods. Differentiation by FLAP method demonstrated a limited similarity to IS6110-RFLP (37,7%). As much as 78,7% of strains were grouped identically when differentiated by FLiP and IS6110-RFLP methods. The analysis differentiated 31, 35, and 36 groups when using FLAP, FLiP, and RFLP-IS6110methods, respectively.

scholarly journals Complete Genome Sequences of Three Representative Mycobacterium tuberculosis Beijing Family Strains Belonging to Distinct Genotype Clusters in Hanoi, Vietnam, during 2007 to 2009

2017 ◽  
Vol 5 (27) ◽  
Author(s):  
Takayuki Wada ◽  
Minako Hijikata ◽  
Shinji Maeda ◽  
Nguyen Thi Le Hang ◽  
Pham Huu Thuong ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Complete Genome ◽  
Population Based ◽  
Beijing Family ◽  
Genome Sequences ◽  
Population Based Study ◽  
Content Type ◽  
Asian Region ◽  
Distinct Genotype ◽  
Reference Strains

ABSTRACT We present here three complete genome sequences of Mycobacterium tuberculosis Beijing family strains isolated in Hanoi, Vietnam. These three strains were selected from major genotypic clusters (15-MIRU-VNTR) identified in a previous population-based study. We emphasize their importance and potential as reference strains in this Asian region.

scholarly journals Epidemiological Analysis of Mycobacterium tuberculosis Strains Isolated from Patients of Small Communities Living in the South-East of Poland

2015 ◽  
Vol 64 (3) ◽  
pp. 289-293
Author(s):  
Anna Zaczek ◽  
Iwona Szwaja ◽  
Monika Skiba ◽  
Anna Brzostek ◽  
Czeslaw Puchalski ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Population Size ◽  
Hybridization Technique ◽  
Great Chance ◽  
Epidemiological Analysis ◽  
Small Communities ◽  
Resistance Patterns ◽  
Dna Profiles ◽  
Pcr Techniques

The diversity of Mycobacterium tuberculosis clinical isolates, collected from a single hospital, was analyzed by ligation-mediated PCR techniques: FLiP and FLAP, and hybridization technique, IS6110-RFLP. The isolated strains were divided in terms of location (3 towns of Podkarpackie voivodeship differing in population size) and relationship (8 members of 4 families, each represented by 2 patients). Within each family identical DNA profiles, as well as drug resistance patterns were identified indicating a great chance of transmission of strains within the same family. Identical, or very similar patterns were also shared by strains isolated from unrelated patients living in a very small town (1 200 inhabitants) or hospitalized in the same place and time.

scholarly journals Modeling Bacterial Evolution with Comparative-Genome-Based Marker Systems: Application to Mycobacterium tuberculosis Evolution and Pathogenesis

2003 ◽  
Vol 185 (11) ◽  
pp. 3392-3399 ◽  
Author(s):  
David Alland ◽  
Thomas S. Whittam ◽  
Megan B. Murray ◽  
M. Donald Cave ◽  
Manzour H. Hazbon ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Population Based ◽  
Mycolic Acid ◽  
Genetic Distances ◽  
Snp Markers ◽  
Comparative Genomic ◽  
Sequence Evolution ◽  
Single Nucleotide ◽  
Comparative Genome ◽  
Reference Strains

ABSTRACT The comparative-genomic sequencing of two Mycobacterium tuberculosis strains enabled us to identify single nucleotide polymorphism (SNP) markers for studies of evolution, pathogenesis, and epidemiology in clinical M. tuberculosis. Phylogenetic analysis using these “comparative-genome markers” (CGMs) produced a highly unusual phylogeny with a complete absence of secondary branches. To investigate CGM-based phylogenies, we devised computer models to simulate sequence evolution and calculate new phylogenies based on an SNP format. We found that CGMs represent a distinct class of phylogenetic markers that depend critically on the genetic distances between compared “reference strains.” Properly distanced reference strains generate CGMs that accurately depict evolutionary relationships, distorted only by branch collapse. Improperly distanced reference strains generate CGMs that distort and reroot outgroups. Applying this understanding to the CGM-based phylogeny of M. tuberculosis, we found evidence to suggest that this species is highly clonal without detectable lateral gene exchange. We noted indications of evolutionary bottlenecks, including one at the level of the PHRI “C” strain previously associated with particular virulence characteristics. Our evidence also suggests that loss of IS6110 to fewer than seven elements per genome is uncommon. Finally, we present population-based evidence that KasA, an important component of mycolic acid biosynthesis, develops G312S polymorphisms under selective pressure.

scholarly journals Survival and Replication of Clinical Mycobacterium tuberculosis Isolates in the Context of Human Innate Immunity

2005 ◽  
Vol 73 (5) ◽  
pp. 2595-2601 ◽  
Author(s):  
Ernestas Janulionis ◽  
Carolina Sofer ◽  
Stephan K. Schwander ◽  
Denarra Nevels ◽  
Barry Kreiswirth ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Mycobacterium Tuberculosis ◽  
Blood Culture ◽  
Whole Blood ◽  
Host Response ◽  
Host Cells ◽  
Clinical Isolates ◽  
The Face ◽  
Mycobacterial Growth ◽  
Reference Strains

ABSTRACT The initial host response to Mycobacterium tuberculosis is driven by innate immunity. For this study, we examined the ability of 18 recent clinical isolates and 5 reference strains to survive and replicate in the context of host innate immunity by using whole blood culture. Six healthy tuberculin-negative volunteers served as subjects. H37Ra showed the least capacity to replicate of any of the strains tested, decreasing in viability 1.3 log CFU during 72 h of whole blood culture, whereas H37Rv increased 0.32 log. Clinical isolates varied greatly in their ability to replicate in blood cells, ranging from −0.4 to +0.8 log (P < 0.001). Four showed significantly more growth than H37Rv, and one showed significantly reduced growth. Host mechanisms for restricting intracellular mycobacterial growth were more effective during the first 24 h of whole blood culture than during the 24- to 72-h period. Certain mycobacterial isolates appeared preferentially able to withstand host defenses during each of these intervals. Although there was relatively more homogeneity among subjects than among strains, one of the six subjects showed a reduced capacity to restrict intracellular mycobacterial growth due to a defect expressed during the first 24 h of culture. Our findings indicate substantial variability in the capacity of clinical tuberculosis isolates to replicate in host cells in the face of innate host immunity.

Epidemiological analysis of Mycobacterium tuberculosis strains isolated in Lodz, Poland

2011 ◽  
Vol 15 (9) ◽  
pp. 1252-1258 ◽  
Author(s):  
M. Krawczyk ◽  
A. Brzostek ◽  
A. Gorna ◽  
K. Knapska ◽  
M. Ziolkiewicz ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Epidemiological Analysis
Sign in / Sign up

Export Citation Format

Share Document