Analysis of amino acids using paper chromatography (Horizontal migration)

1952 ◽  
Vol 36 (5) ◽  
Author(s):  
P. S. Rao ◽  
R. M. Beri
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Horizontal Migration

PAPER CHROMATOGRAPHY OF COMPLEX BIOLOGICAL MATERIALS BY SOLVENT REDEVELOPMENT WITHOUT PRIOR REMOVAL OF SALTS OR PROTEINS

1960 ◽  
Vol 38 (10) ◽  
pp. 1137-1147 ◽  
Author(s):  
Arthur E. Pasieka
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper ◽  
Complex Mixtures ◽  
Two Dimensional ◽  
One Dimensional ◽  
Equivalent Time ◽  
Biological Compounds ◽  
High Concentrations ◽  
Subsequent Staining

A solvent redeveloping technique has been devised by which amino acids, peptides, and sugars can be separated from complex mixtures in the presence of high concentrations of salts and proteins. The separations are effected by two to four successive 18-hour solvent developments with drying between each 18-hour period before subsequent staining of the chromatograms. Better separations and resolutions are obtained by such successive 18-hour solvent developments than by one continuous solvent development for an equivalent time. The effect of these redevelopments on the separations and resolutions of biological compounds is illustrated at various stages by photographs of one- and two-dimensional chromatograms. The redevelopment technique requires filter paper sheets up to 4 ft in length for one-dimensional analytical and preparative types of chromatograms.

PAPER CHROMATOGRAPHY OF THE AMINO ACIDS OF CEREBROSPINAL FLUID

1954 ◽  
Vol 58 (5) ◽  
pp. 595-601 ◽  
Author(s):  
Burnham S. Walker ◽  
Norman Telles ◽  
Edward Pastore
Keyword(s):  
Amino Acids ◽  
Cerebrospinal Fluid ◽  
Paper Chromatography

Paper chromatography of urinary amino acids

1956 ◽  
Vol 1 (1) ◽  
pp. 75-79 ◽  
Author(s):  
Jorge Awapara ◽  
Yukiko Sato
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Urinary Amino Acids ◽  
Urinary Amino

RESOLUTION INTO OPTICAL ISOMERS OF SOME AMINO ACIDS BY PAPER CHROMATOGRAPHY

1951 ◽  
Vol 73 (6) ◽  
pp. 2973-2974 ◽  
Author(s):  
M. Kotake ◽  
T. Sakan ◽  
N. Nakamura ◽  
S. Senoh
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Optical Isomers

QUANTITATIVE CHROMATOGRAPHIC METHODS: PART 7. ISOLATION OF AMINO ACIDS FROM SERUM AND OTHER FLUIDS

1961 ◽  
Vol 39 (2) ◽  
pp. 439-451 ◽  
Author(s):  
C. K. Harris ◽  
E. Tigane ◽  
C. S. Hanes
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Ion Exchange ◽  
Paper Chromatography ◽  
Biological Fluids ◽  
Inorganic Cations ◽  
Chromatographic Methods ◽  
Tissue Extracts

A method based on the use of miniature ion-exchange columns has been devised for isolating small amounts of amino acids from biological fluids and tissue extracts. The amino acids are isolated virtually free from proteins, sugars, and inorganic cations in a single treatment.Techniques are described for the preparation of solutions of the isolated amino acids for quantitative analysis by paper chromatography.

Use of n-Butanol - Formic Acid - Water Mixture in the Paper Chromatography of Amino-Acids and Sugars

Nature ◽  
1952 ◽  
Vol 170 (4320) ◽  
pp. 279-280 ◽  
Author(s):  
L. F. WIGGINS ◽  
J. HOWARTH WILLIAMS
Keyword(s):  
Amino Acids ◽  
Formic Acid ◽  
Paper Chromatography ◽  
Acid Water ◽  
Water Mixture
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