Modulation of coregulatory protein expression in human breast cancer

2005 ◽  
Vol 174 (S1) ◽  
pp. 22-22
Author(s):  
D. O. Kavanagh ◽  
A. D. K. Hill ◽  
E. W. M. McDermott ◽  
N. J. O’Higgins ◽  
L. S. Young
Keyword(s):  
Breast Cancer ◽  
Protein Expression ◽  
Human Breast Cancer ◽  
Human Breast ◽  
Coregulatory Protein

scholarly journals c-erbB-4 protein expression in human breast cancer

2000 ◽  
Vol 82 (6) ◽  
pp. 1163-1170 ◽  
Author(s):  
T Y Kew ◽  
J A Bell ◽  
S E Pinder ◽  
H Denley ◽  
R Srinivasan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Protein Expression ◽  
Human Breast Cancer ◽  
Human Breast

Long Noncoding RNA LINC00261 Induces Chemosensitization to Tamoxifen in Human Breast Cancer

2020 ◽  
Vol 10 (6) ◽  
pp. 789-797
Author(s):  
Zhaoyan Shi ◽  
Weidong Xiao ◽  
Meifang Hu
Keyword(s):  
Breast Cancer ◽  
Protein Expression ◽  
Human Breast Cancer ◽  
Noncoding Rna ◽  
Quantitative Polymerase Chain Reaction ◽  
Chemotherapy Resistance ◽  
Migration And Invasion ◽  
Human Breast ◽  
Mcf 7

Breast cancer (BC) is one of the most prevalent and mortal malignancies in women worldwide, and tamoxifen is the mainstay treatment of breast cancer and the development of resistance represents a major obstacle for a cure. Long non-coding RNAs (LncRNAs) LINC00261 have been identified to serve a key role in the development of several tumors. However, the role of LINC00261 in breast cancer and chemotherapy resistance remains largely unknown. To investigate the role of LINC00261 in BC cells, LINC00261 was upregulated in MCF-7-TAM cells by transfecting with LINC00261 plasmid (pcDNA-LINCC00261). Subsequently, cell viability and drug sensitivity were measured using the CCK-8 assay. Reverse transcription-quantitative polymerase chain reaction (qRT-PCR) was performed to detect the level of LINC00261 in BC cells. Cell migration, invasion, and apoptosis were detected by Transwell, Scratch Test and Flow cytometry, respectively. Additionally, the associated protein expression was detected using Western blot. The results demonstrated that LINC00261 was significantly down-regulated in BC cells, especially in MCF-7-TAM cells. Overexpression of LINC00261 inhibited cell proliferation, migration, and invasion in MCF-7-TAM cells. Further, an abundant of LINC00261 sensitized breast cancer cells to tamoxifen and reduced tamoxifen-induced apoptosis in MCF-7-TAM cells. Finally, LINC00261 significantly regulated the protein expression of drug-resistant genes and the protein expression related to tumor metastasis and cell apoptosis. Therefore, this study revealed that LINC00261 induces chemosensitization to tamoxifen in human breast cancer, it may be a useful biomarker and potential therapeutic target.

Cyclin D1 protein expression and function in human breast cancer

1994 ◽  
Vol 57 (3) ◽  
pp. 353-361 ◽  
Author(s):  
Jirina Bartkova ◽  
Jiri Lukas ◽  
Heiko Müller ◽  
Dorrit Lützhøt ◽  
Michael Strauss ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Protein Expression ◽  
Cyclin D1 ◽  
Human Breast Cancer ◽  
D1 Protein ◽  
Human Breast ◽  
Cyclin D1 Protein ◽  
And Function

Different distributions of immunoreactive S100-α and S100-β protein expression in human breast cancer

1998 ◽  
Vol 68 (1) ◽  
pp. 25-29 ◽  
Author(s):  
Hiroomi Funahashi ◽  
Takashi Koshikawa ◽  
Shu Ichihara ◽  
Ekoh Ohike ◽  
Kanehusa Katoh
Keyword(s):  
Breast Cancer ◽  
Protein Expression ◽  
Human Breast Cancer ◽  
Human Breast ◽  
Β Protein

Progesterone receptor A and B protein expression in human breast cancer

1996 ◽  
Vol 56 (1-6) ◽  
pp. 93-98 ◽  
Author(s):  
J. Dinny Graham ◽  
Christine Yeates ◽  
Rosemary L. Balleine ◽  
Suzanna S. Harvey ◽  
Jane S. Milliken ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Progesterone Receptor ◽  
Protein Expression ◽  
Human Breast Cancer ◽  
Human Breast

Histone deacetylase-1 and -3 protein expression in human breast cancer: a tissue microarray analysis

2005 ◽  
Vol 90 (1) ◽  
pp. 15-23 ◽  
Author(s):  
Claudia A. Krusche ◽  
Pia W�lfing ◽  
Christian Kersting ◽  
Anne Vloet ◽  
Werner B�cker ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Protein Expression ◽  
Histone Deacetylase ◽  
Tissue Microarray ◽  
Microarray Analysis ◽  
Human Breast Cancer ◽  
Human Breast ◽  
Histone Deacetylase 1 ◽  
Tissue Microarray Analysis

scholarly journals Screening to Identify Commonly Used Chinese Herbs That AffectERBB2andESR1Gene Expression Using the Human Breast Cancer MCF-7 Cell Line

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Jen-Hwey Chiu ◽  
Chun-Ju Chang ◽  
Jing-Chong Wu ◽  
Hui-Ju Liu ◽  
Che-Sheng Wen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Protein Expression ◽  
Cell Line ◽  
Human Breast Cancer ◽  
Luciferase Activity ◽  
Chinese Herbs ◽  
Herbal Extracts ◽  
Human Breast ◽  
Mcf 7

Aim. Our aim the was to screen the commonly used Chinese herbs in order to detect changes inERBB2andESR1gene expression using MCF-7 cells.Methods. Using the MCF-7 human breast cancer cell line, cell cytotoxicity and proliferation were evaluated by MTT and trypan blue exclusion assays, respectively. A luciferase reporter assay was established by transient transfecting MCF-7 cells with plasmids containing either theERBB2or theESR1promoter region linked to the luciferase gene. Chinese herbal extracts were used to treat the cells at 24 h after transfection, followed by measurement of their luciferase activity. The screening results were verified by Western blotting to measure HER2 and ERαprotein expression.Results. At concentrations that induced little cytotoxicity, thirteen single herbal extracts and five compound recipes were found to increase eitherERBB2orESR1luciferase activity. By Western blotting, Si-Wu-Tang, Kuan-Shin-Yin, and Suan-Tsao-Ren-Tang were found to increase either HER2 or ERαprotein expression. In addition,Ligusticum chuanxiongwas shown to have a great effect onERBB2gene expression and synergistically with estrogen to stimulate MCF-7 cell growth.Conclusion. Our results provide important information that should affect clinical treatment strategies among breast cancer patients who are receiving hormonal or targeted therapies.

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