Assessment of the micronucleus assay as a biological dosimeter using cytokinesis-blocked lymphocytes from cancer patients receiving fractionated partial body-radiotherapy

1998 ◽  
Vol 174 (2) ◽  
pp. 75-81 ◽  
Author(s):  
J. le Roux ◽  
J. Slabbert ◽  
B. Smit ◽  
G. Blekkenhorst
Keyword(s):  
Cancer Patients ◽  
Micronucleus Assay ◽  
Partial Body

Radiation Morbidity in Cancer Patients May Be Predicted by the Micronucleus Assay

2005 ◽  
Vol 63 ◽  
pp. S486-S487
Author(s):  
T. Lee ◽  
R.R. Allison ◽  
K.F. O′Brien ◽  
R.M. Johnke ◽  
U.L. Karlsson ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Micronucleus Assay

scholarly journals Biological Dosimetry Using Micronucleus Assay in Simulated Partial-Body Exposure to Ionizing Radiation

2017 ◽  
Vol 43 (2) ◽  
pp. 75 ◽  
Author(s):  
S. Purnami ◽  
S. Nurhayati ◽  
M. Syaifudin ◽  
D. Ramadhani
Keyword(s):  
Ionizing Radiation ◽  
Micronucleus Assay ◽  
Biological Dosimetry ◽  
Partial Body

Correlation between Mitotic Delay and Micronucleus Assay in Breast cancer patients

2008 ◽  
Vol 68 (S 01) ◽  
Author(s):  
DP Varga ◽  
H Surowy ◽  
W Vogel ◽  
Z Atassi ◽  
R Kreienberg ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Micronucleus Assay ◽  
Breast Cancer Patients ◽  
Mitotic Delay

scholarly journals Molecular contribution of BRCA1 and BRCA2 to genome instability in breast cancer patients: review of radiosensitivity assays

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Fatemeh Sadeghi ◽  
Marzieh Asgari ◽  
Mojdeh Matloubi ◽  
Maral Ranjbar ◽  
Nahid Karkhaneh Yousefi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Dna Repair ◽  
Cancer Patients ◽  
Micronucleus Assay ◽  
Breast Cancer Patients ◽  
Brca1 And Brca2 ◽  
Repair Capacity ◽  
Dna Repair Capacity ◽  
History Of

Abstract Background DNA repair pathways, cell cycle arrest checkpoints, and cell death induction are present in cells to process DNA damage and prevent genomic instability caused by various extrinsic and intrinsic ionizing factors. Mutations in the genes involved in these pathways enhances the ionizing radiation sensitivity, reduces the individual’s capacity to repair DNA damages, and subsequently increases susceptibility to tumorigenesis. Body BRCA1 and BRCA2 are two highly penetrant genes involved in the inherited breast cancer and contribute to different DNA damage pathways and cell cycle and apoptosis cascades. Mutations in these genes have been associated with hypersensitivity and genetic instability as well as manifesting severe radiotherapy complications in breast cancer patients. The genomic instability and DNA repair capacity of breast cancer patients with BRCA1/2 mutations have been analyzed in different studies using a variety of assays, including micronucleus assay, comet assay, chromosomal assay, colony-forming assay, γ -H2AX and 53BP1 biomarkers, and fluorescence in situ hybridization. The majority of studies confirmed the enhanced spontaneous & radiation-induced radiosensitivity of breast cancer patients compared to healthy controls. Using G2 micronucleus assay and G2 chromosomal assay, most studies have reported the lymphocyte of healthy carriers with BRCA1 mutation are hypersensitive to invitro ionizing radiation compared to non-carriers without a history of breast cancer. However, it seems this approach is not likely to be useful to distinguish the BRCA carriers from non-carrier with familial history of breast cancer. Conclusion In overall, breast cancer patients are more radiosensitive compared to healthy control; however, inconsistent results exist about the ability of current radiosensitive techniques in screening BRCA1/2 carriers or those susceptible to radiotherapy complications. Therefore, developing further radiosensitivity assay is still warranted to evaluate the DNA repair capacity of individuals with BRCA1/2 mutations and serve as a predictive factor for increased risk of cancer mainly in the relatives of breast cancer patients. Moreover, it can provide more evidence about who is susceptible to manifest severe complication after radiotherapy.

The Role of Micronucleus Assay to Detect Genetic Instability in Respiratory Cancer Patients

2019 ◽  
Vol 38 (4) ◽  
pp. 345-352
Author(s):  
Claudia Bolognesi ◽  
Marco Bruzzone ◽  
Vincenzo Fontana ◽  
Donatella Ugolini ◽  
Andrea Compalati ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Genetic Instability ◽  
Micronucleus Assay ◽  
Respiratory Cancer
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