Sero-epidemiological studies of epstein-barr virus (EBV) infection by testing antibodies against EBV specific dnase (EDAb) as a method for early detection of nasopharyngeal carcinoma (NPC)

1997 ◽  
Vol 9 (3) ◽  
pp. 195-202
Author(s):  
Di Huang ◽  
Haifeng Chen ◽  
Luling Cheng ◽  
Siming Fang ◽  
Jianshan Ma ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Early Detection ◽  
Epstein Barr Virus ◽  
Epidemiological Studies ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

scholarly journals Early detection of nasopharyngeal carcinoma by plasma Epstein-Barr virus DNA analysis in a surveillance program

Cancer ◽  
2013 ◽  
Vol 119 (10) ◽  
pp. 1838-1844 ◽  
Author(s):  
K. C. Allen Chan ◽  
Emily C. W. Hung ◽  
John K. S. Woo ◽  
Paul K. S. Chan ◽  
Sing-Fai Leung ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Early Detection ◽  
Epstein Barr Virus ◽  
Dna Analysis ◽  
Surveillance Program ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals The initial study of EBNA-2 polymorphisms in nasopharyngeal carcinoma in Vietnam

2020 ◽  
Vol 8 (1) ◽  
pp. 60-67
Author(s):  
Ngo Dong Kha ◽  
Huynh Thi Mong Tuyen ◽  
Lam Hong Ngoc ◽  
Thieu Hong Hue ◽  
Le Quang Anh Tuan ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Initial Data ◽  
Epstein Barr Virus ◽  
Initial Study ◽  
Nucleotide Sequencing ◽  
Potential Contribution ◽  
Barr Virus ◽  
Ebv Infection ◽  
Vietnamese Population ◽  
Epstein Barr

Epstein-Barr virus (EBV) infection is the main cause of Nasopharyngeal Carcinoma (NPC). EBNA-2, one of the most important genes participating in the formation of NPC, also helps EBV evade an attack on the immune system. EBNA-2 has 4 variants including E2-A, E2-B, E2-C and E-2D, of which E2-A and E2-C are the characterized variants for NPC. This study aimed to evaluate the variations of EBNA-2 in NPC biopsy samples of Vietnamese patients. This initial study used 10 biopsy samples, which were positively confirmed to NPC, collected from Cho Ray Hospital. Nested PCR – nucleotide sequencing was applied to analyze the variants of EBNA-2. The results showed that 8 out of 10 samples, accounting for 80%, were positive to EBNA-2. Additionally, only two variants, E-2A and E-2C were detected in our study, in which, E2-A subtype was identified as the predominant subtype. These findings would provide initial data about potential contribution of EBNA-2 polymorphisms to etiology of NPC in Vietnamese population.

scholarly journals Epstein-Barr Virus Mediated Signaling in Nasopharyngeal Carcinoma Carcinogenesis

Cancers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2441 ◽  
Author(s):  
Timmy Richardo ◽  
Pongphol Prattapong ◽  
Chawalit Ngernsombat ◽  
Nurulfitri Wisetyaningsih ◽  
Hisashi Iizasa ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Southeast Asia ◽  
Signaling Pathways ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Ebv Infection ◽  
Non Coding Rnas ◽  
Epstein Barr ◽  
Exact Relationship

Nasopharyngeal carcinoma (NPC) is one of the most common tumors occurring in China and Southeast Asia. Etiology of NPC seems to be complex and involves many determinants, one of which is Epstein-Barr virus (EBV) infection. Although evidence demonstrates that EBV infection plays a key role in NPC carcinogenesis, the exact relationship between EBV and dysregulation of signaling pathways in NPC needs to be clarified. This review focuses on the interplay between EBV and NPC cells and the corresponding signaling pathways, which are modulated by EBV oncoproteins and non-coding RNAs. These altered signaling pathways could be critical for the initiation and progression of NPC.

scholarly journals Early Detection of Nasopharyngeal Carcinoma

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Keiji Tabuchi ◽  
Masahiro Nakayama ◽  
Bungo Nishimura ◽  
Kentaro Hayashi ◽  
Akira Hara
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Early Detection ◽  
Epstein Barr Virus ◽  
Clinical Presentation ◽  
Southern China ◽  
Squamous Cell Carcinomas ◽  
Treatment Results ◽  
Barr Virus ◽  
Associated Malignancy ◽  
Epstein Barr

Nasopharyngeal carcinoma (NPC) is a unique disease with a clinical presentation, epidemiology, and histopathology differing from other squamous cell carcinomas of the head and neck. NPC is an Epstein-Barr virus-associated malignancy with a marked racial and geographic distribution. Specifically, it is highly prevalent in southern China, Southeast Asia, and the Middle East. To date, most NPC patients have been diagnosed in the advanced stage, but the treatment results for advanced NPC are not satisfactory. This paper provides a brief overview regarding NPC, with the focus on the early detection of initial and recurrent NPC lesions.

scholarly journals Link of Nasopharyngeal Carcinoma and Epstein-Barr Virus

2018 ◽  
Vol 7 (2) ◽  
pp. 51-55
Author(s):  
Sugiyanto Sugiyanto ◽  
Lina Aryati ◽  
Fajar Adi Kusumo ◽  
Mardiah Suci Hardianti
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Carcinoma Cells ◽  
Lymphoid Cells ◽  
Epithelial Tissue ◽  
Barr Virus ◽  
Ebv Infection ◽  
Ebv Dna ◽  
Epstein Barr ◽  
Basic Level

Nasopharyngeal Carcinoma (NPC) is a cancer that occurs in nasopharynx which is associated with Epstein-Barr Virus (EBV). Mutation agents in nasopharyngeal neoplasms occur because of EBV infection. Transformation of B-cells due to EBV causes hormone imbalance in lymphoid cells or nasopharyngeal epithelial tissue. Rates of EBV infection have been shown to be prognostic to NPC. The basic level of EBV DNA can be used for stratification prognosis, with higher titers showing greater disease severity and worse outcomes. With mathematical models, there is a correlation between the increase in Epstein-Barr Virus and the increase in Invasive Carcinoma Cells or increase in Nasopharyngeal Carcinoma Cells.

scholarly journals Methylation Marker has a Complementary Value to Epstein-Barr Virus-Based Tests in Early Detection of Nasopharyngeal Carcinoma

2012 ◽  
Vol 23 ◽  
pp. ix88
Author(s):  
S.H. Hutajulu ◽  
S.R. Indrasari ◽  
L.P.L. Indrawati ◽  
A. Brahmadhi ◽  
S. Duin ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Early Detection ◽  
Epstein Barr Virus ◽  
Methylation Marker ◽  
Barr Virus ◽  
Epstein Barr ◽  
Complementary Value

scholarly journals Role of Epstein-Barr virus in the development of nasopharyngeal carcinoma

Open Medicine ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 171-176 ◽  
Author(s):  
Hui Zhang ◽  
Jing Wang ◽  
Dan Yu ◽  
Yan Liu ◽  
Kai Xue ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Southern China ◽  
Latent Membrane Protein 1 ◽  
Barr Virus ◽  
Ebv Infection ◽  
Micro Rnas ◽  
Total Cancer ◽  
Epstein Barr

AbstractSouthern China experiences larger extent of total cancer pathologies, of which nasopharyngeal carcinoma has the highest incidence under otorhinolaryngeal malignant carcinomas. Risk factor of nasopharyngeal carcinoma varies from hereditary causes to virus infection, among which Epstein-Barr virus (EBV) infection is the mostly investigated. The study into mechanism of EBV in occurrence, development and prognosis of nasopharyngeal carcinoma has been studied for several decades. The pathophysiology in making of EBV into a cancerogen includes proteins as latent membrane protein 1 (LMPs) and nucleic acids as micro-RNAs. In this paper, we reviewed till date studies focusing on relationship between EBV and nasopharyngeal carcinoma.

scholarly journals Regulation of DNA Damage Signaling and Cell Death Responses by Epstein-Barr Virus Latent Membrane Protein 1 (LMP1) and LMP2A in Nasopharyngeal Carcinoma Cells

2015 ◽  
Vol 89 (15) ◽  
pp. 7612-7624 ◽  
Author(s):  
Laura R. Wasil ◽  
Leizhen Wei ◽  
Christopher Chang ◽  
Li Lan ◽  
Kathy H. Y. Shair
Keyword(s):  
Dna Damage ◽  
Cell Death ◽  
Dna Repair ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Combined Effects ◽  
Barr Virus ◽  
Ebv Infection ◽  
Dna Damage Signaling ◽  
Epstein Barr

ABSTRACTNasopharyngeal carcinoma (NPC) is closely associated with latent Epstein-Barr virus (EBV) infection. Although EBV infection of preneoplastic epithelial cells is not immortalizing, EBV can modulate oncogenic and cell death mechanisms. The viral latent membrane proteins 1 (LMP1) and LMP2A are consistently expressed in NPC and can cooperate in bitransgenic mice expressed from the keratin-14 promoter to enhance carcinoma development in an epithelial chemical carcinogenesis model. In this study, LMP1 and LMP2A were coexpressed in the EBV-negative NPC cell line HK1 and examined for combined effects in response to genotoxic treatments. In response to DNA damage activation, LMP1 and LMP2A coexpression reduced γH2AX (S139) phosphorylation and caspase cleavage induced by a lower dose (5 μM) of the topoisomerase II inhibitor etoposide. Regulation of γH2AX occurred before the onset of caspase activation without modulation of other DNA damage signaling mediators, including ATM, Chk1, or Chk2, and additionally was suppressed by inducers of DNA single-strand breaks (SSBs) and replication stress. Despite reduced DNA damage repair signaling, LMP1-2A coexpressing cells recovered from cytotoxic doses of etoposide; however, LMP1 expression was sufficient for this effect. LMP1 and LMP2A coexpression did not enhance cell growth, with a moderate increase of cell motility to fibronectin. This study supports that LMP1 and LMP2A jointly regulate DNA repair signaling and cell death activation with no further enhancement in the growth properties of neoplastic cells.IMPORTANCENPC is characterized by clonal EBV infection and accounts for >78,000 annual cancer cases with increased incidence in regions where EBV is endemic, such as southeast Asia. The latent proteins LMP1 and LMP2A coexpressed in NPC can individually enhance growth or survival properties in epithelial cells, but their combined effects and potential regulation of DNA repair and checkpoint mechanisms are relatively undetermined. In this study, LMP1-2A coexpression suppressed activation of the DNA damage response (DDR) protein γH2AX induced by selective genotoxins that promote DNA replication stress or SSBs. Expression of LMP1 was sufficient to recover cells, resulting in outgrowth of LMP1 and LMP1-2A-coexpressing cells and indicating distinct LMP1-dependent effects in the restoration of replicative potential. These findings demonstrate novel properties for LMP1 and LMP2A in the cooperative modulation of DDR and apoptotic signaling pathways, further implicating both proteins in the progression of NPC and epithelial malignancies.

scholarly journals Epstein-Barr Virus Latent Membrane Protein-1 Expression in Nasopharyngeal Carcinoma

2021 ◽  
pp. 1406-1412
Author(s):  
Valerie E. Salano ◽  
Amos R. Mwakigonja ◽  
Ashfaq Abdulshakoor ◽  
Aveline A. Kahinga ◽  
Enica M. Richard
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Membrane Protein ◽  
Epstein Barr Virus ◽  
Latent Membrane Protein ◽  
Latent Membrane Protein 1 ◽  
Female Ratio ◽  
Histologic Subtype ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

PURPOSE Nasopharyngeal carcinoma (NPC), a malignant neoplasm of the epithelium covering the nasopharynx, is a rare disease in most parts of the world. Epstein-Barr virus (EBV), the most potent oncogenic virus, coupled with environmental and genetic factors has been identified to play a role in the development of NPC. An array of methods for detecting the virus do exist, from serologic detection of antibodies to DNA amplification. There is paucity of local data on the status of EBV infection in relation to NPC within the region, and this study attempts to shed more light on the subject. METHODS This was a retrospective cross-sectional laboratory-based study on histologically confirmed, archived tissues from July 2015 to June 2019. Immunohistochemistry expression of latent membrane protein-1 (LMP-1) was used to detect EBV infection in the tissues. RESULTS A total of 71 cases were enrolled in this study. The mean age was 47.87 years ± 16.84 years with a male-to-female ratio of 1.5:1. There was a unimodal distribution of EBV detection, with the peak (26.8%) at 36-45 years. About 45.1% of the 71 samples tested positive for LMP-1, all of which were nonkeratinizing carcinoma. Nonkeratinizing carcinoma was the most common histopathologic subtype (n = 67; 94.4%), with the majority (38 of 67; 56.7%) being undifferentiated and 29 of 67 (43.3%) differentiated. Keratinizing and basaloid subtypes had two cases each, representing 2.8%. CONCLUSION A significant proportion of NPC, particularly nonkeratinizing histologic subtype, seems to show LMP-1 positivity by immunohistochemistry, which may be adopted in resource-constrained settings to detect EBV infection in these tissue biopsies.

scholarly journals Faktor-faktor penyebab terjadinya karsinoma nasofaring (KNF)

2020 ◽  
Vol 2 (1) ◽  
pp. 113-120
Author(s):  
Nur Suci Amanah
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cells ◽  
Epstein Barr Virus ◽  
Dietary Habits ◽  
Common Disease ◽  
Salted Fish ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr ◽  
Nasopharyngeal Epithelial

Nasopharyngeal carcinoma is a common disease in southern China. The etiologies of the main factors proposed for the pathogenesis of KNF include genetic factors, environmental factors and Epstein Barr Virus (EBV) infection. Other causes besides preserved food consumption include salted fish which have been involved in the etiology of NPC. The downward trend in the incidence of NPC has occurred in Hong Kong for the past 20 years, which is caused by changes in dietary habits. Despite the close relationship of EBV infection with NPC, the etiological role of EBV in the pathogenesis of NPC remains an interaction. EBV infection in primary nasopharyngeal epithelial cells occurs. Epstein Barr virus does not convert primary nasopharyngeal epithelial cells into proliferative clones, which is in sharp contrast to the well-documented ability of EBV to alter and perpetuate primary B cells. Genetic changes that are supported in the nasopharyngeal epithelium may be needed to support stable EBV infection. Non-viral factors as a cause of nasopharyngeal carcinoma still cannot be resolved with certainty. Non-viral factors are one of the risk factors that can increase the number of events arising from nasopharyngeal malignancies such as smoke, salted fish, formaldehyde, genetic, as soon as possible firewood, wood dust, chronic infection, throat protector, alcohol and traditional medicine.

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