Microalgal biotechnology: Carotenoid production by the green algaeDunaliella salina

2003 ◽  
Vol 8 (6) ◽  
pp. 331-337 ◽  
Author(s):  
Eon Seon Jin ◽  
Anastasios Melis
Keyword(s):  
Carotenoid Production ◽  
Microalgal Biotechnology

scholarly journals Transcriptome Analysis Reveals a Promotion of Carotenoid Production by Copper Ions in Recombinant Saccharomyces cerevisiae

2021 ◽  
Vol 9 (2) ◽  
pp. 233
Author(s):  
Buli Su ◽  
Anzhang Li ◽  
Ming-Rong Deng ◽  
Honghui Zhu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcriptome Analysis ◽  
Gene Expression Analysis ◽  
Target Genes ◽  
Copper Ions ◽  
Carotenoid Production ◽  
Carotenoid Accumulation ◽  
Nutritional Components ◽  
Differential Gene ◽  
First Time

We previously constructed a Saccharomyces cerevisiae carotenoid producer BL03-D-4 which produced much more carotenoid in YPM (modified YPD) media than YPD media. In this study, the impacts of nutritional components on carotenoid accumulation of BL03-D-4 were investigated. When using YPM media, the carotenoid yield was increased 10-fold compared to using the YPD media. To elucidate the hidden mechanism, a transcriptome analysis was performed and showed that 464 genes changed significantly in YPM media. Furthermore, inspired by the differential gene expression analysis which indicated that ADY2, HES1, and CUP1 showed the most remarkable changes, we found that the improvement of carotenoid accumulation in YPM media was mainly due to the copper ions, since supplementation of 0.08 mM CuSO4 in YPD media could increase carotenoid yield 9.2-fold. Reverse engineering of target genes was performed and carotenoid yield could be increased 6.4-fold in YPD media through overexpression of ACE1. The present study revealed for the first time the prominent promotion of carotenoid yield by copper ions in engineered S. cerevisiae and provided a new target ACE1 for genetic engineering of S. cerevisiae for the bioproduction of carotenoids.

scholarly journals Sustainable development of microalgal biotechnology in coastal zone for aquaculture and food

2021 ◽  
pp. 146369
Author(s):  
Xiangning Lu ◽  
Yulin Cui ◽  
Yuting Chen ◽  
Yupeng Xiao ◽  
Xiaojin Song ◽  
...  
Keyword(s):  
Sustainable Development ◽  
Coastal Zone ◽  
Microalgal Biotechnology

scholarly journals Evaluation of olive mill waste as substrate for carotenoid production by Rhodotorula mucilaginosa

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Carolina Ghilardi ◽  
Paola Sanmartin Negrete ◽  
Amalia Antonia Carelli ◽  
Virginia Borroni
Keyword(s):  
Liquid Fraction ◽  
Added Value ◽  
Organic Load ◽  
Integral Approach ◽  
Rhodotorula Mucilaginosa ◽  
Total Sugars ◽  
Carotenoid Production ◽  
Total Sugar Concentration ◽  
Valuable Compounds ◽  
Olive Mill

Abstract The “alperujo” is a waste from the olive oil industry with great potential for valorization. It has a high organic load, with the presence of valuable compounds such as biophenols and sugars. The use of this waste can be thought of as a biorefinery from which different compounds of high added value can be obtained, whether they are present in the “alperujo” such as biophenols or can be generated from the “alperujo”. Therefore, the production of carotenoids by Rhodotorula mucilaginosa was evaluated using the liquid fraction of ‘alperujo’ (Alperujo Water, AW) or an aqueous extract (AE) of “alperujo” at different concentrations (5, 10, 20 and 30% w/V) as substrates. The AEs had an acidic pH, a total sugar concentration ranging from 1.6 to 7.6 g/L, a polyphenols content from 0.4 to 2.9 g/L and a significant amount of proteins (0.5–3 g/L). AW is similar in composition as 30% AE, but with a higher amount of total sugars. Rh. mucilaginosa was able to grow at the different mediums with consumption of glucose and fructose, a reduction in protein content and alkalinization of the medium. Maximum total carotenoid production (7.3 ± 0.6 mg/L) was achieved at AW, while the specific production was higher when the yeast grew at AW or at 30% AE (0.78 ± 0.06 and 0.73 ± 0.10 mg/g of biomass, respectively). Torulene and torularhodin were the main carotenoids produced. Polyphenol content did not change; thus, it is still possible to recover these compounds after producing carotenoids. These results demonstrate the feasibility of using alperujo-based mediums as cheap substrates to produce torularhodin and torulene and to include this bioprocess as a step in an integral approach for alperujo valorization.

Responses of Rhodotorula mucilaginosa under Pb( II ) stress: carotenoid production and budding

2021 ◽  
Author(s):  
Zhijun Wang ◽  
Ying Zhang ◽  
Liu Jiang ◽  
Jingjing Qiu ◽  
Yanan Gao ◽  
...  
Keyword(s):  
Rhodotorula Mucilaginosa ◽  
Carotenoid Production

Carotenoid Production by Filamentous Fungi and Yeasts

2017 ◽  
pp. 225-279 ◽  
Author(s):  
Javier Avalos ◽  
Steffen Nordzieke ◽  
Obdulia Parra ◽  
Javier Pardo-Medina ◽  
M. Carmen Limón
Keyword(s):  
Filamentous Fungi ◽  
Carotenoid Production

Coordinate expression of multiple bacterial carotenoid genes in canola leading to altered carotenoid production

2003 ◽  
Vol 5 (4) ◽  
pp. 255-263 ◽  
Author(s):  
Monica P Ravanello ◽  
Dangyang Ke ◽  
Julie Alvarez ◽  
Bihua Huang ◽  
Christine K Shewmaker
Keyword(s):  
Carotenoid Production ◽  
Coordinate Expression ◽  
Carotenoid Genes

Carotenoid production from Chlorococcum littorale in photoautotrophic cultures with downstream supercritical fluid processing

2009 ◽  
Vol 32 (13) ◽  
pp. 2327-2335 ◽  
Author(s):  
Masaki Ota ◽  
Hiromoto Watanabe ◽  
Yoshitaka Kato ◽  
Masaru Watanabe ◽  
Yoshiyuki Sato ◽  
...  
Keyword(s):  
Supercritical Fluid ◽  
Carotenoid Production ◽  
Supercritical Fluid Processing
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