Pilot scale production of poly(3-hydroxybutyrate-co-3-hydroxy-valerate) by fed-batch culture of recombinantEscherichia coli

2002 ◽  
Vol 7 (6) ◽  
pp. 371-374 ◽  
Author(s):  
Jong-il Choi ◽  
Sang Yup Lee ◽  
Kyungsup Shin ◽  
Woo Gi Lee ◽  
Si Jae Park ◽  
...  
Keyword(s):  
Batch Culture ◽  
Pilot Scale ◽  
Scale Production ◽  
Fed Batch ◽  
Pilot Scale Production ◽  
Fed Batch Culture

Optimization of the pilot-scale production of an ice-binding protein by fed-batch culture of Pichia pastoris

2012 ◽  
Vol 97 (8) ◽  
pp. 3383-3393 ◽  
Author(s):  
Jun Hyuck Lee ◽  
Sung Gu Lee ◽  
Hackwon Do ◽  
Jong Chan Park ◽  
Eunjung Kim ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Batch Culture ◽  
Binding Protein ◽  
Pilot Scale ◽  
Scale Production ◽  
Fed Batch ◽  
Ice Binding ◽  
Pilot Scale Production ◽  
Ice Binding Protein ◽  
Fed Batch Culture

scholarly journals Segmented linear modeling of CHO fed-batch culture and its application to large scale production

2016 ◽  
Vol 114 (4) ◽  
pp. 785-797 ◽  
Author(s):  
Bassem Ben Yahia ◽  
Boris Gourevitch ◽  
Laetitia Malphettes ◽  
Elmar Heinzle
Keyword(s):  
Batch Culture ◽  
Large Scale ◽  
Scale Production ◽  
Fed Batch ◽  
Linear Modeling ◽  
Large Scale Production ◽  
Fed Batch Culture

scholarly journals Pilot-Scale Production of Chito-Oligosaccharides Using an Innovative Recombinant Chitosanase Preparation Approach

Polymers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 290
Author(s):  
Chih-Yu Cheng ◽  
Chia-Huang Tsai ◽  
Pei-Jyun Liou ◽  
Chi-Hang Wang
Keyword(s):  
Cell Density ◽  
Scale Up ◽  
Cost Effective ◽  
Pilot Scale ◽  
Ionic Concentration ◽  
Dihydrogen Phosphate ◽  
Scale Production ◽  
Sodium Dihydrogen Phosphate ◽  
Selective Precipitation ◽  
Pilot Scale Production

For pilot-scale production of chito-oligosaccharides, it must be cost-effective to prepare designable recombinant chitosanase. Herein, an efficient method for preparing recombinant Bacillus chitosanase from Escherichia coli by elimination of undesirable substances as a precipitate is proposed. After an optimized culture with IPTG (Isopropyl β-d-1-thiogalactopyranoside) induction, the harvested cells were resuspended, disrupted by sonication, divided by selective precipitation, and stored using the same solution conditions. Several factors involved in these procedures, including ion types, ionic concentration, pH, and bacterial cell density, were examined. The optimal conditions were inferred to be pH = 4.5, 300 mM sodium dihydrogen phosphate, and cell density below 1011 cells/mL. Finally, recombinant chitosanase was purified to >70% homogeneity with an activity recovery and enzyme yield of 90% and 106 mg/L, respectively. When 10 L of 5% chitosan was hydrolyzed with 2500 units of chitosanase at ambient temperature for 72 h, hydrolyzed products having molar masses of 833 ± 222 g/mol with multiple degrees of polymerization (chito-dimer to tetramer) were obtained. This work provided an economical and eco-friendly preparation of recombinant chitosanase to scale up the hydrolysis of chitosan towards tailored oligosaccharides in the near future.

Pilot-scale production of cloudy juice from low-quality pear fruit under low-oxygen conditions

2015 ◽  
Vol 173 ◽  
pp. 827-837 ◽  
Author(s):  
Domien De Paepe ◽  
Katleen Coudijzer ◽  
Bart Noten ◽  
Dirk Valkenborg ◽  
Kelly Servaes ◽  
...  
Keyword(s):  
Pilot Scale ◽  
Scale Production ◽  
Low Oxygen ◽  
Pear Fruit ◽  
Pilot Scale Production ◽  
Oxygen Conditions
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