Construction of two new vectors for transformation of laboratory, natural and industrialSaccharomyces cerevisiae strains to trifluoroleucine and G418 resistance

2004 ◽  
Vol 49 (5) ◽  
pp. 534-538 ◽  
Author(s):  
I. Bardazzi ◽  
E. Casalone
Keyword(s):  
G418 Resistance

Isolation of microcell hybrid clones containing retroviral vector insertions into specific human chromosomes

1987 ◽  
Vol 7 (8) ◽  
pp. 2814-2820
Author(s):  
T G Lugo ◽  
B Handelin ◽  
A M Killary ◽  
D E Housman ◽  
R E Fournier
Keyword(s):  
High Efficiency ◽  
Human Fibroblasts ◽  
Retroviral Vector ◽  
Human Chromosomes ◽  
G418 Resistance ◽  
Microcell Hybrid ◽  
Dominant Selectable Marker ◽  
Hybrid Clones ◽  
Microcell Fusion

We sought an efficient means to introduce specific human chromosomes into stable interspecific hybrid cells for applications in gene mapping and studies of gene regulation. A defective amphotropic retrovirus was used to insert the gene conferring G418 resistance (neo), a dominant selectable marker, into the chromosomes of diploid human fibroblasts, and the marked chromosomes were transferred to mouse recipient cells by microcell fusion. We recovered five microcell hybrid clones containing one or two intact human chromosomes which were identified by karyotype and marker analysis. Integration of the neo gene into a specific human chromosome in four hybrid clones was confirmed by segregation analysis or by in situ hybridization. We recovered four different human chromosomes into which the G418 resistance gene had integrated: human chromosomes 11, 14, 20, and 21. The high efficiency of retroviral vector transformation makes it possible to insert selectable markers into any mammalian chromosomes of interest.

A stable bovine papillomavirus hybrid plasmid that expresses a dominant selective trait

1983 ◽  
Vol 3 (11) ◽  
pp. 2110-2115
Author(s):  
M F Law ◽  
J C Byrne ◽  
P M Howley
Keyword(s):  
Resistance Gene ◽  
Mammalian Cell ◽  
Morphological Characteristics ◽  
Hybrid Plasmid ◽  
Transcriptional Unit ◽  
Bovine Papillomavirus ◽  
G418 Resistance ◽  
Extrachromosomal Element ◽  
Mouse Cells ◽  
Neomycin Resistance

We describe a bovine papillomavirus hybrid plasmid containing the neomycin resistance gene from Tn5 inserted into a mammalian cell transcriptional unit. This plasmid is maintained as a stable extrachromosomal element (20 to 100 copies per diploid genome) in mouse cells selected either for the transformed phenotype or for resistance to the aminoglycoside G418. Cells selected for G418 resistance initially display a flat, nontransformed phenotype before exhibiting the gross morphological characteristics of transformation. The delay in the appearance of the transformed phenotype indicated that some intracellular event or series of events subsequent to the establishment of transcriptionally active bovine papillomavirus 1 hybrid plasmid is required for the manifestation of the transformed phenotype.

scholarly journals Improved mammalian vectors for high expression of G418 resistance.

1987 ◽  
Vol 12 (6) ◽  
pp. 575-580 ◽  
Author(s):  
Kazuto Katoh ◽  
Yoshiko Takahashi ◽  
Sigeo Hayashi ◽  
Hisato Kondoh
Keyword(s):  
High Expression ◽  
G418 Resistance

Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn905

Gene ◽  
1984 ◽  
Vol 28 (1) ◽  
pp. 73-81 ◽  
Author(s):  
K. Sreekrishna ◽  
Thomas D. Webster ◽  
Robert C. Dickson
Keyword(s):  
Resistance Gene ◽  
Kluyveromyces Lactis ◽  
G418 Resistance

scholarly journals Hygromycin resistance as a selectable marker in Dictyostelium discoideum.

1989 ◽  
Vol 9 (5) ◽  
pp. 1965-1968 ◽  
Author(s):  
T T Egelhoff ◽  
S S Brown ◽  
D J Manstein ◽  
J A Spudich
Keyword(s):  
Dictyostelium Discoideum ◽  
High Efficiency ◽  
Selectable Marker ◽  
Hygromycin Resistance ◽  
Origin Of Replication ◽  
Transcription Terminator ◽  
G418 Resistance ◽  
Hygromycin Selection ◽  
Hygromycin Resistance Gene ◽  
Transformed Cell Lines

We have constructed an expression cartridge which has the bacterial hygromycin resistance gene (hph) fused to the Dictyostelium discoideum actin 15 promoter, with a segment of 3'-flanking DNA from the actin 15 locus placed downstream of the hph gene to serve as a transcription terminator. The plasmid pDE109, which contained this cartridge and a Dictyostelium origin of replication, transformed D. discoideum with high efficiency under hygromycin selection. The availability of this selectable marker circumvents the previous limitation of having G418 resistance as the only selectable marker for this organism; secondary transformation can now be used to introduce DNA into previously transformed cell lines.

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