Characterization of extracellular polysaccharides fromNostoc flagelliforme cells in liquid suspension culture

2007 ◽  
Vol 12 (3) ◽  
pp. 271-275 ◽  
Author(s):  
Shiru Jia ◽  
Haifeng Yu ◽  
Yongxian Lin ◽  
Yujie Dai
Keyword(s):  
Suspension Culture ◽  
Extracellular Polysaccharides ◽  
Liquid Suspension ◽  
Liquid Suspension Culture

scholarly journals Studies on lolium multiflorum endosperm in tissue culture I. Nutrition

1973 ◽  
Vol 26 (1) ◽  
pp. 123 ◽  
Author(s):  
M MeryI Smith ◽  
BA Stone
Keyword(s):  
Tissue Culture ◽  
Suspension Culture ◽  
Doubling Time ◽  
Lolium Multiflorum ◽  
Carbon Sources ◽  
Equimolar Mixture ◽  
Liquid Suspension ◽  
The Mean ◽  
Liquid Suspension Culture

Stocks of L. multiflorum endosperm callus have been maintained in liquid suspension culture on a modified White's medium for 5 years. The mean doubling time under the conditions used is 3� 2 days. Best growth is obtained on sucrose; fructose and glucose are good carbon sources, whereas growth is only moderate on an equimolar mixture of both.

scholarly journals TEMPERATURE-SENSITIVE VARIANTS OF NICOTIANA TABACUM ISOLATED FROM SOMATIC CELL CULTURE

Genetics ◽  
1979 ◽  
Vol 92 (1) ◽  
pp. 215-221
Author(s):  
Russell L Malmberg
Keyword(s):  
Nicotiana Tabacum ◽  
Suspension Culture ◽  
Selection Procedure ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Permissive Temperature ◽  
Temperature Growth ◽  
Liquid Suspension ◽  
Slow Growing ◽  
Liquid Suspension Culture

ABSTRACT Temperature-sensitive variants of Nicotiana tabacum were isolated from a liquid suspension culture of somatic cells by a negative selection procedure, using bromodeoxyuridine and light. A total of nine such variants have been recovered, with an estimated rate of 2 × 10-7 per cell division. The appearance of the variants at the permissive temperature varied from nearly wild type, white and friable, to brown, compact and slow growing. Two of the variants adapted from growth on solid medium to growth in a liquid suspension culture; these were further characterized for chromosome number, growth rate, cell death rate at the restrictive temperature, growth on nutritionally modified media, and RNA and protein synthesis. The variants have been placed on regeneration media, and one of them has produced plantlets. Leaves from a plantlet have been placed on callus-inducing media, and the resulting callus displayed the temperature-sensitive phenotype.

All-Trans Retinoic Acid Delays the Differentiation of Primitive Hematopoietic Precursors (lin−c-kit+Sca-1+) While Enhancing the Terminal Maturation of Committed Granulocyte/Monocyte Progenitors

Blood ◽  
1999 ◽  
Vol 94 (2) ◽  
pp. 483-495 ◽  
Author(s):  
Louise E. Purton ◽  
Irwin D. Bernstein ◽  
Steven J. Collins
Keyword(s):  
Retinoic Acid ◽  
Suspension Culture ◽  
Terminal Differentiation ◽  
Potent Inducer ◽  
Cell Production ◽  
Trans Retinoic Acid ◽  
Liquid Suspension ◽  
All Trans Retinoic Acid ◽  
Colony Forming Cell ◽  
Liquid Suspension Culture

All-trans retinoic acid (ATRA) is a potent inducer of terminal differentiation of malignant promyelocytes, but its effects on more primitive hematopoietic progenitors and stem cells are less clear. In this study, we investigated the effect of ATRA on highly enriched murine hematopoietic precursor cells (lin−c-kit+Sca-1+) grown in liquid suspension culture for 28 days. ATRA initially slowed the growth of these hematopoietic precursors but prolonged and markedly enhanced their colony-forming cell production compared with the hematopoietic precursors cultured in its absence. At 7 and 14 days of culture, a substantially greater percentage of cells cultured with ATRA did not express lineage-associated antigens (55.4% at day 7 and 68.6% at day 14) and retained expression of Sca-1 (44.7% at day 7 and 79.9% at day 14) compared with cells grown in its absence (lin−cells: 31.5% at day 7 and 4% at day 14; Sca-1+: 10.4% at day 7 and 0.7% at day 14). Moreover, a marked inhibition of granulocyte production was observed in cultures continuously incubated with ATRA. Significantly, ATRA markedly prolonged and enhanced the production of transplantable colony-forming unit-spleen (CFU-S) during 14 days of liquid suspension culture. In contrast with its effects on primitive lin−c-kit+Sca-1+hematopoietic precursors, ATRA did not exert the same effects on the more committed lin−c-kit+Sca-1−progenitor cells. Moreover, the late addition of ATRA (7 days post-culture initiation) to cultures of primitive hematopoietic precursors resulted in a marked decrease in colony-forming cell production in these cultures, which was associated with enhanced granulocyte differentiation. These observations indicate that ATRA has different effects on hematopoietic cells depending on their maturational state, preventing and/or delaying the differentiation of primitive hematopoietic precursors while enhancing the terminal differentiation of committed granulocyte/monocyte progenitors.

scholarly journals Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Bo-Kook Jang ◽  
Ju-Sung Cho ◽  
Shin-Ho Kang ◽  
Cheol Hee Lee
Keyword(s):  
Suspension Culture ◽  
Culture Method ◽  
Culture Period ◽  
Pteridium Aquilinum ◽  
Fresh Weight ◽  
High Accumulation ◽  
Liquid Suspension ◽  
Immature Development ◽  
Endogenous Phytohormone ◽  
Liquid Suspension Culture

Abstract Background Liquid suspension culture efficiently proliferates plant cells and can be applied to ferns because it rapidly increases the fresh weight of gametophytes. This study assessed gametophyte proliferation and sporophyte production of Pteridium aquilinum var. latiusculum using a suspension culture method. Results The growth curve linear phase of gametophyte cells was confirmed between 9 and 18 days of culture, and the subculture cycle was determined to be 2 weeks. A double-strength MS medium (fresh weight, 18.0 g) containing 2% sucrose and NH4+:NO3− (120 mM, 40:80) was found to be the optimal liquid medium. Gametophytes obtained after suspension culture for 18 days did not normally form sporophytes in an ex vitro soil environment. However, this issue was resolved after changing the culture type or extending the culture period to 6 weeks. A short suspension culture period increased the fresh weight of fragmented and homogenized gametophytes but yielded numerous relatively immature gametophytes (globular forms of branching gametophytes, BG). Furthermore, differences in gametophyte morphogenesis and development were indicated by changes in endogenous phytohormone content. BG with immature development exhibited high accumulation of zeatin, jasmonic acid, and salicylic acid, and relatively low levels of abscisic acid and indole-3-acetic acid. The immature development of gametophytes directly affected sporophyte formation. Conclusions This study maximized the advantages of liquid suspension culture using eastern bracken gametophytes and provides data to resolve any associated issues, thus facilitating efficient bracken production.

Sign in / Sign up

Export Citation Format

Share Document