Synergistic effects of stem cell factor and interleukin 6 or interleukin 11 on the expansion of murine hematopoietic progenitors in liquid suspension culture

Stem Cells ◽  
1995 ◽  
Vol 13 (4) ◽  
pp. 404-413 ◽  
Author(s):  
Yuko Ariyama ◽  
Shinichi Misawa ◽  
Yoshiaki Sonoda
Keyword(s):  
Stem Cell ◽  
Suspension Culture ◽  
Interleukin 6 ◽  
Stem Cell Factor ◽  
Synergistic Effects ◽  
Hematopoietic Progenitors ◽  
Liquid Suspension ◽  
Interleukin 11 ◽  
Liquid Suspension Culture

scholarly journals Hematopoietic stem cells in the blood after stem cell factor and interleukin-11 administration: evidence for different mechanisms of mobilization

Blood ◽  
1995 ◽  
Vol 86 (12) ◽  
pp. 4674-4680 ◽  
Author(s):  
P Mauch ◽  
C Lamont ◽  
TY Neben ◽  
C Quinto ◽  
SJ Goldman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Progenitor Cells ◽  
Stem Cell Factor ◽  
Cytotoxic Agents ◽  
Peripheral Blood Stem Cells ◽  
Hematopoietic Stem ◽  
Blood Stem Cells ◽  
Interleukin 11

Peripheral blood stem cells and progenitor cells, collected during recovery from exposure to cytotoxic agents or after cytokine administration, are being increasingly used in clinical bone marrow transplantation. To determine factors important for mobilization of both primitive stem cells and progenitor cells to the blood, we studied the blood and splenic and marrow compartments of intact and splenectomized mice after administration of recombinant human interleukin-11 (rhlL-11), recombinant rat stem cell factor (rrSCF), and IL-11 + SCF. IL-11 administration increased the number of spleen colony- forming units (CFU-S) in both the spleen and blood, but did not increase blood long-term marrow-repopulating ability (LTRA) in intact or splenectomized mice. SCF administration increased the number of CFU- S in both the spleen and blood and did not increase the blood or splenic LTRA of intact mice, but did increase blood LTRA to normal marrow levels in splenectomized mice. The combination of lL-11 + SCF syngeristically enhanced mobilization of long-term marrow-repopulating cells from the marrow to the spleen of intact mice and from the marrow to the blood of splenectomized mice. These data, combined with those of prior studies showing granulocyte colony-stimulating factor mobilization of long-term marrow repopulating cells from the marrow to the blood of mice with intact spleens, suggest different cytokine- induced pathways for mobilization of primitive stem cells.

scholarly journals Recruitment of primitive peripheral blood cells: synergism of interleukin 12 with interleukin 6 and stem cell factor

1999 ◽  
Vol 105 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Stephanie Grafte-Faure ◽  
Catherine Leveque ◽  
Marc Vasse ◽  
Claudine Soria ◽  
Jean-Pierre Vannier
Keyword(s):  
Stem Cell ◽  
Interleukin 6 ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Stem Cell Factor ◽  
Interleukin 12 ◽  
Peripheral Blood Cells

scholarly journals Autocrine insulin-like growth factor 1 and stem cell factor but not interleukin 6 support self-renewal of human myeloma cells

2013 ◽  
Vol 3 (6) ◽  
pp. e120-e120 ◽  
Author(s):  
D Chiron ◽  
S Maïga ◽  
S Surget ◽  
G Descamps ◽  
P Gomez-Bougie ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Interleukin 6 ◽  
Stem Cell Factor ◽  
Insulin Like Growth Factor ◽  
Self Renewal ◽  
Myeloma Cells ◽  
Human Myeloma Cells

scholarly journals Mechanisms of Stem Cell Factor and Erythropoietin Proliferative Co-signaling in FDC2-ER Cells

Blood ◽  
1997 ◽  
Vol 90 (9) ◽  
pp. 3533-3545 ◽  
Author(s):  
Bhavana Joneja ◽  
Hong-Chi Chen ◽  
Dhaya Seshasayee ◽  
Amy L. Wrentmore ◽  
Don M. Wojchowski
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Stem Cell Factor ◽  
Progenitor Cell ◽  
Egf Receptor ◽  
Synergistic Effects ◽  
Ectopic Expression ◽  
Hematopoietic Progenitor Cell ◽  
Detectable Effect ◽  
Epo Receptor

Abstract Studies of hematopoietic progenitor cell development in vivo, ex vivo, and in factor-dependent cell lines have shown that c-kit promotes proliferation through synergistic effects with at least certain type 1 cytokine receptors, including the erythropoietin (Epo) receptor. Presently, c-kit is shown to efficiently support both mitogenesis and survival in the FDCP1 cell subline, FDC2. In this system, mitogenic synergy with c-kit was observed for ectopically expressed wild-type Epo receptors (wt-ER), an epidermal growth factor (EGF) receptor/Epo receptor chimera, and a highly truncated Epo receptor construct ER-Bx1. Thus, the Epo receptor cytoplasmic box 1 subdomain appears, at least in part, to mediate mitogenic synergy with c-kit. In studies of potential effectors of this response, Jak2 tyrosine phosphorylation was shown to be induced by Epo, but not by stem cell factor (SCF). In addition and in contrast to signaling in Mo7e and BM6 cell lines, in FDC2-ER cells SCF and Epo each were shown to rapidly activate Pim 1 gene expression. Recently, roles also have been suggested for the nuclear trans-factor GATA-1 in regulating progenitor cell proliferation. In FDC2-ER cells, the ectopic expression of GATA-1 had no detectable effect on Epo inhibition of apoptosis. However, GATA-1 expression did result in a selective and marked inhibition in mitogenic responsiveness to SCF and to a decrease in c-kit transcript expression. These studies of SCF and Epo signaling in FDC2–wt-ER cells serve to functionally map the ERB1 region as a c-kit–interactive domain, suggest that Pim1 might contribute to SCF and Epo mitogenic synergy and support the notion that SCF and Epo may act in opposing ways during red cell differentiation.

scholarly journals Synergistic actions of stem cell factor and other burst-promoting activities on proliferation of CD34+ highly purified blood progenitors expressing HLA-DR or different levels of c-kit protein

Blood ◽  
1994 ◽  
Vol 84 (12) ◽  
pp. 4099-4106 ◽  
Author(s):  
Y Sonoda ◽  
H Sakabe ◽  
Y Ohmisono ◽  
S Tanimukai ◽  
S Yokota ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Early Stage ◽  
Human Peripheral Blood ◽  
Synergistic Effects ◽  
Target Population ◽  
Additive Effect ◽  
Gm Csf ◽  
Stage Of Development ◽  
Hla Dr

We studied the synergistic effects of stem cell factor (SCF) and other burst-promoting activities (BPAs) such as interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), or IL-9 on proliferation of human peripheral blood-derived highly purified progenitors. SCF, IL-3, GM-CSF, and IL-9 showed significant BPA when CD34+HLA-DR+ cells were used as the target population. IL-3 exerted the most potent BPA, and GM-CSF supported approximately 40% to 70% of the erythroid burst-forming units that are responsive to IL-3. SCF and IL-9 showed much weaker BPA than that of IL-3 or GM-CSF. Combinations of IL- 3 with other BPAs did not show synergistic actions supporting erythroid- burst formation. However, GM-CSF showed a significant additive effect with IL-9 or SCF. When CD34+c-kithigh cells were used as the target, SCF showed a much stronger BPA. Also, a distinct additive effect between SCF and IL-3 or GM-CSF on erythrocyte-containing mixed colony formation was observed. On the other hand, when CD34+c-kitlow cells were used as the target, SCF, IL-3, and GM-CSF could express BPA. In contrast, IL-9 alone failed to support erythroid-burst formation. Because CD34+c-kithigh cells weakly expressed CD34 antigen, these cells appeared to be more mature progenitors than CD34+c-kitlow cells. These results suggest that IL-9 acts on more mature progenitors than those of SCF, IL-3, or GM-CSF and that the primary target of SCF is multipotential progenitors at the very early stage of development.

Eotaxin Modulates Myelopoiesis and Mast Cell Development From Embryonic Hematopoietic Progenitors

Blood ◽  
1998 ◽  
Vol 92 (6) ◽  
pp. 1887-1897 ◽  
Author(s):  
Elizabeth J. Quackenbush ◽  
Barry K. Wershil ◽  
Vincent Aguirre ◽  
Jose-Carlos Gutierrez-Ramos
Keyword(s):  
Mast Cell ◽  
Stem Cell ◽  
Mast Cells ◽  
Stem Cell Factor ◽  
Fetal Liver ◽  
Hematopoietic Progenitors ◽  
Embryonic Tissues ◽  
Pathological Conditions ◽  
Gi Protein ◽  
American Society

Abstract Eotaxin is a potent chemoattractant for eosinophils during inflammation and allergic reactions in the adult, but its role in the embryonic development of the hematopoietic system has not been examined. We report here that eotaxin and its receptor, CCR-3, are expressed by embryonic tissues responsible for blood development, such as fetal liver (FL), yolk sac (YS), and peripheral blood. We found that eotaxin acts synergistically with stem cell factor to accelerate the differentiation of embryonic mast cell progenitors, and this response can be suppressed by pertussis toxin, an inhibitor of chemokine-induced signaling through Gi protein and chemotaxis. Eotaxin promotes the differentiation of fetal mast cell progenitors into differentiated mast cells as defined by the expression of mast cell specific proteases. Furthermore, in combination with stem cell factor (SCF), it promotes the growth of Mac-1+myeloid cells from embryonic progenitors. These studies suggest that eotaxin may be involved in the growth of granulocytic progenitors and the differentiation and/or function of mast cells during embryogenesis and/or pathological conditions that induce high levels of eotaxin, such as allergic responses. © 1998 by The American Society of Hematology.

scholarly journals Ex vivo expansion with stem cell factor and interleukin-11 augments both short-term recovery posttransplant and the ability to serially transplant marrow

Blood ◽  
1996 ◽  
Vol 87 (11) ◽  
pp. 4589-4595 ◽  
Author(s):  
TL Holyoake ◽  
MG Freshney ◽  
L McNair ◽  
AN Parker ◽  
PJ McKay ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor ◽  
Ex Vivo ◽  
Culture Conditions ◽  
Ex Vivo Expansion ◽  
Short Term ◽  
Ex Vivo Culture ◽  
Interleukin 11 ◽  
Transplantation Model

The characterization of many cytokines involved in the control of hematopoiesis has led to intense investigation into their potential use in ex vivo culture to expand progenitor numbers. We have established the optimum ex vivo culture conditions that allow substantial amplification of transient engrafting murine stem cells and which, simultaneously, augment the ability to sustain serial bone marrow transplantation (BMT). Short-term incubation of unfractionated BM cells in liquid culture with stem cell factor (SCF) and interleukin-11 (IL- 11) produced a 50-fold amplification of clonogenic multipotential progenitors (CFU-A). Following such ex vivo expansion, substantially fewer cells were required to rescue lethally irradiated mice. When transplanted in cell doses above threshold for engraftment, BM cells expanded ex vivo resulted in significantly more rapid hematopoietic recovery. In a serial transplantation model, unmanipulated BM was only able to consistently sustain secondary BMT recipients, but BM expanded ex vivo has sustained quaternary BMT recipients that remain alive and well more than 140 days after 4th degree BMT. These results show augmentation of both short-term recovery posttransplant and the ability to serially transplant marrow by preincubation in culture with SCF and IL-11.

scholarly journals Studies on lolium multiflorum endosperm in tissue culture I. Nutrition

1973 ◽  
Vol 26 (1) ◽  
pp. 123 ◽  
Author(s):  
M MeryI Smith ◽  
BA Stone
Keyword(s):  
Tissue Culture ◽  
Suspension Culture ◽  
Doubling Time ◽  
Lolium Multiflorum ◽  
Carbon Sources ◽  
Equimolar Mixture ◽  
Liquid Suspension ◽  
The Mean ◽  
Liquid Suspension Culture

Stocks of L. multiflorum endosperm callus have been maintained in liquid suspension culture on a modified White's medium for 5 years. The mean doubling time under the conditions used is 3� 2 days. Best growth is obtained on sucrose; fructose and glucose are good carbon sources, whereas growth is only moderate on an equimolar mixture of both.

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