Determination of residual water in freeze-dried bacterial cultures by Fischer’s method

1962 ◽  
Vol 7 (4) ◽  
pp. 247-252 ◽  
Author(s):  
J. FabiÀn ◽  
L. ŠindelÀŘ
Keyword(s):  
Residual Water ◽  
Bacterial Cultures ◽  
Freeze Dried

Reduction of Potassium in Kidney Proximal Tubules to Aid in Electron Probe X-Ray Microanalysis of Calcium

1985 ◽  
Vol 43 ◽  
pp. 474-475
Author(s):  
A. LeFurgey ◽  
P. Ingram ◽  
L.J. Mandel
Keyword(s):  
Smooth Muscle ◽  
Proximal Tubule ◽  
Electron Probe ◽  
Clinical Applications ◽  
Proximal Tubules ◽  
Rabbit Kidney ◽  
Target Cells ◽  
X Ray ◽  
Freeze Dried

For quantitative determination of subcellular Ca distribution by electron probe x-ray microanalysis, decreasing (and/or eliminating) the K content of the cell maximizes the ability to accurately separate the overlapping K Kß and Ca Kα peaks in the x-ray spectra. For example, rubidium has been effectively substituted for potassium in smooth muscle cells, thus giving an improvement in calcium measurements. Ouabain, a cardiac glycoside widely used in experimental and clinical applications, inhibits Na-K ATPase at the cell membrane and thus alters the cytoplasmic ion (Na,K) content of target cells. In epithelial cells primarily involved in active transport, such as the proximal tubule of the rabbit kidney, ouabain rapidly (t1/2= 2 mins) causes a decrease2 in intracellular K, but does not change intracellular total or free Ca for up to 30 mins. In the present study we have taken advantage of this effect of ouabain to determine the mitochondrial and cytoplasmic Ca content in freeze-dried cryosections of kidney proximal tubule by electron probe x-ray microanalysis.

Determination of reservoir rock residual water using X-ray computed microtomography

2018 ◽  
pp. 38-42 ◽  
Author(s):  
I.V. Yazynina ◽  
◽  
E.V. Shelyago ◽  
A.A. Abrosimov ◽  
N.E. Grachev ◽  
...  
Keyword(s):  
Reservoir Rock ◽  
Residual Water ◽  
X Ray ◽  
Computed Microtomography ◽  
X Ray Computed

Colorimetric Determination of Hydroxyproline as Measure of Collagen Content in Meat and Meat Products: NMKL Collaborative Study

1990 ◽  
Vol 73 (1) ◽  
pp. 54-57 ◽  
Author(s):  
Kurt Kolar
Keyword(s):  
Collaborative Study ◽  
Colorimetric Method ◽  
Meat Products ◽  
Acid Oxidation ◽  
Colorimetric Determination ◽  
Mean Values ◽  
Collaborative Studies ◽  
Freeze Dried ◽  
Analytical Result

Abstract A colorimetric method for the determination of hydroxyproline as a measure of collagen in meat and meat products has been collaboratively studied in 18 laboratories. The method includes hydrolysis with sulfuric acid, oxidation with chloramine- T, and formation of a reddish purple complex with 4- dimethylaminobenzaldehyde. Five frozen and 3 freeze-dried samples were tested, ranging in content from 0.11 to 0.88% and from 0.39 to 4.0% hydroxyproline, respectively. The mean values of 2 identical samples were 0.245 and 0.251 %. The average recovery from a spiked sample was 96.1 %. The hydroxyproline content of a known sample (a mixture of 2 samples in the ratio 5:2) was calculated to 1.42%, which agrees well with the analytical result, 1.40%. In comparison with other collaborative studies, based on the ISO analytical method, the repeatability and reproducibility of this method agree well with the other results. This method was accepted as an official NMKL method by all national Committees, and has been adopted official first action by AOAC as an NMKLAOAC method.

scholarly journals Assessment and determination of lyoprotectant for survival of freeze-dried Lactobacillus rhamnosus

2016 ◽  
Vol 20 (1) ◽  
pp. 105-113
Author(s):  
Zongcheng Miao ◽  
Yang Zhao ◽  
Xiaoping Huo
Keyword(s):  
Amino Acids ◽  
Fractional Factorial Design ◽  
Biological Activities ◽  
Lactobacillus Rhamnosus ◽  
Fractional Factorial ◽  
Practical Application ◽  
Steepest Ascent ◽  
Freeze Dried ◽  
Viable Bacteria

Abstract Currently research of lactic acid bacteria focus primarily on the functional probiotics, which are major beneficial biota in the gastrointestinal tract, have been industrial manufactured. Probiotics confer health benefits on the host need adequate amounts. However, the absence of data makes it difficult to ensure the maintenance biological activities and population of probiotic. In this research, a fractional factorial design and steepest ascent experiment were used to analyze the influence of lyoprotectant as carbohydrates, prebiotics and amino acids on the survival of the probiotic Lactobacillus rhamnosus. The results indicated a maximum survival rate and population of viable bacteria of L. rhamnosus to be 55.84 % and 1.60 ×1011 CFU/g after freeze-dried by using a combination of 10 g/100mL Sucrose, 2.5 g/100mL Isomaltooligosaccharide, 12 g/100mL Hydroxyproline. To a large extent, the survival and viability were dependent on the cryoprotectant used and make probiotics more attractive from a practical application in industrial viewpoint.

Determination of readiness for laying based on material moisture, corresponding relative humidity, and water release

2020 ◽  
Vol 62 (10) ◽  
pp. 1033-1040
Author(s):  
Christoph Strangfeld ◽  
Sabine Kruschwitz
Keyword(s):  
Relative Humidity ◽  
Moisture Content ◽  
Embedded Sensors ◽  
Residual Water ◽  
Floor Covering ◽  
Water Release ◽  
Content Determination ◽  
Two Samples ◽  
Moisture Content Determination

Abstract The moisture content of the subfloor has to be determined before installation to avoid damage to the floor covering. Only if readiness for layering is reached, can an installation without damage be expected in all cases. In general, three approaches exist to measure residual water content: determination of moisture content, determination of water release, or determination of the corresponding relative humidity. All three approaches are tested under laboratory conditions at eight screed types including two samples thicknesses in each case. Moisture content and water release are measured by sample weighing, the corresponding relative humidity is measured by embedded sensors. All three approaches are compared and correlated. The evaluations show only a weak correlation and, in several cases, contradicting results. Samples are considered ready for layering and not ready for layering at the same time, depending on the chosen approach. Due to these contradicting results, a general threshold for a risk of damage cannot be derived based on these measurements. Furthermore, the experiment demonstrates that the measurement of corresponding relative humidity is independent of the screed type or screed composition considered. This makes humidity measurement a potentially very promising approach for the installation of material moisture monitoring systems.

Determination of the minimum suppressive concentration of photosensitizers of the second generation in relation to sanitaryindicative microorganisms

2021 ◽  
Vol 1 (11) ◽  
pp. 42-46
Author(s):  
Vadim Yu. Shlenskiy ◽  
◽  
Tatiana I. Pashnik ◽  
Svetlana Yu. Pigina ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Salmonella Enteritidis ◽  
Second Generation ◽  
Research Work ◽  
Protoporphyrin Ix ◽  
Bactericidal Effect ◽  
Disodium Salt ◽  
Bacterial Cultures

In the studies described in the article, the presence of antimicrobial activity in photosensitizers in experiments on bacterial cultures was confirmed. In the experiments, photosensitizers of the second generation were used: «Photoditazine», the active substance of which is dimeglumin chloride E6, and «Dimegin», which includes a derivative of protoporphyrin IX-2,4-di(1-methoxyethyl)- deuteroporphyrin IX disodium salt. In the described research work, the minimum concentrations of the studied drugs were determined by the method of sequential tenfold dilutions to obtain a bactericidal effect on strains of sanitary-indicative microorganisms Enterococcus faecalis, Staphylococcus aureus, Salmonella enteritidis.

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