Persistence and spread of mycoplasma in axenic callus tissue cultures of tobacco (Nicotiana glauca Grah.) in the presence of kinetin and IAA in nutrient medium

1975 ◽  
Vol 17 (5) ◽  
pp. 352-356 ◽  
Author(s):  
Eva Petrů ◽  
Marie Ulrychová
Keyword(s):  
Nutrient Medium ◽  
Callus Tissue ◽  
Tissue Cultures ◽  
Nicotiana Glauca

scholarly journals In vitro assessment of wheat tolerance to drought

Genetika ◽  
2005 ◽  
Vol 37 (2) ◽  
pp. 165-171 ◽  
Author(s):  
Vladislava Galovic ◽  
Zorana Kotaranin ◽  
Srbislav Dencic
Keyword(s):  
Drought Stress ◽  
Nutrient Medium ◽  
Callus Tissue ◽  
Geographic Origin ◽  
Control Group ◽  
Zygotic Embryos ◽  
Fresh Weight ◽  
Effects Of Drought ◽  
In Vitro Effects

Analyzed in this paper were the in vitro effects of drought stress in 13 genotypes of winter wheat, one genotype of spring wheat, and three Triticale genotypes of different geographic origin. Callus tissue was induced from immature zygotic embryos (10-15 days after pollination) on a modified MS nutrient medium. After two weeks, callus tissue was transplanted onto the same medium enriched with 5% high-molecular polyethylene glycol (PEG 6000), which was used as the stress agent to produce the effect of drought chemically. A control group of calluses was grown on an identical medium but without PEG. After four weeks of growing calluses on these mediums, we assessed callus mass survival ability of the genotypes before the transplantation as well as percentage reduction of callus fresh weight after the transplantation onto the nutrient medium with 5% PEG. Statistically significant differences were found among the genotypes in their response to the induced stress. The best survival ability before the transplantation was found in the genotype Mexicol20 (83%), while the lowest was recorded in Slavija (11.3%). Culture growing under stress conditions significantly reduced callus fresh weight in all of the genotypes. The lowest decrease of the callus mass relative to control was recorded in Rozofskaja (14.4%) and the highest in Miranovska (58.4%), indicating the genotypes' tolerance levels towards drought stress.

Inducing effect of plant cells on nitrogenase activity by Spirillum and Rhizobium in vitro

1978 ◽  
Vol 24 (2) ◽  
pp. 143-148 ◽  
Author(s):  
J. J. Child ◽  
W. G. W. Kurz
Keyword(s):  
Plant Cell ◽  
Callus Tissue ◽  
Nitrogenase Activity ◽  
Nutritional Requirement ◽  
Tissue Cultures ◽  
Cell Tissue ◽  
Direct Association ◽  
Pentose Sugar ◽  
Rhizobium Sp

Eleven different plant cell tissue cultures of both legume and non-legume origin have been grown in direct association, and in separate but close proximal association with both Spirillum lipoferum and Rhizobium sp. 32H1. Basic similarities were found in the nutritional requirement for the induction of nitrogenase activity (C2H2) in both organisms. In the absence of plant cell cultures both organisms need to be provided with a pentose sugar and a tricarboxylic acid to induce high levels of nitrogen-fixing activity. Plant cell callus tissue appears only capable of supplying the tricarboxylic acids needed but not the sugar component. The plant tissue, however, seems able to activate certain carbohydrates, which in themselves are incapable of substituting for the pentose additive.

Hydroxyrutacridon-Epoxid, ein neues Acridon-Alkaloid aus Ruta graveolens / Hydroxyrutacridone Epoxide, a New Acridone Alkaloid from Ruta graveolens

1982 ◽  
Vol 37 (1-2) ◽  
pp. 132-133 ◽  
Author(s):  
U. Eilert ◽  
B. Wolters ◽  
A. Nahrstedt ◽  
V. Wray
Keyword(s):  
Callus Tissue ◽  
Spectroscopic Methods ◽  
Tissue Cultures ◽  
Ruta Graveolens ◽  
Acridone Alkaloid

AbstractHydroxyrutacridone epoxide was isolated from roots and callus tissue cultures of Ruta graveolens L. and identified by spectroscopic methods by comparison to rutacridone epoxide.

Shoot production from onion callus tissue cultures

1978 ◽  
Vol 9 (2) ◽  
pp. 99-110 ◽  
Author(s):  
D.I. Dunstan ◽  
K.C. Short
Keyword(s):  
Callus Tissue ◽  
Tissue Cultures ◽  
Shoot Production

Growth Inhibition in Tobacco (Nicotiana tabacum) Callus by 2,6-Dinitroaniline Herbicides and Protection by D-α-tocopherol Acetate

Weed Science ◽  
1978 ◽  
Vol 26 (6) ◽  
pp. 527-530 ◽  
Author(s):  
J. B. Huffman ◽  
N. D. Camper
Keyword(s):  
Regression Analysis ◽  
Nicotiana Tabacum ◽  
Callus Tissue ◽  
Linear Regression Analysis ◽  
Tissue Growth ◽  
Tissue Cultures ◽  
Fresh Weight ◽  
Structural Variations ◽  
Tocopherol Acetate ◽  
Dinitroaniline Herbicides

Tobacco(Nicotiana tabacumL. ‘X-73’) callus tissue cultures were used in a bioassay system for determining the effect of the following substituted 2,6-dinitroaniline herbicides on growth: trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine); oryzalin (3,5-dinitro-N4,N4-dipropylsulfanilamide); benefin(N-butyl-N-ethyl-α,α,α-trifluoro-2,6-dinitro-p-toluidine); AC 92390(N-sec-butyl-2,6-dinitro-3,4-xylidine); penoxalin [N-(1-ethylpropyl)-2,6-dinitro-3,4-xylidine]; GS-38946(N-ethyl-N-tetrahydrofurfuryl-4-trifluoromethyl-2,6-dinitroaniline); chlornidine [N,N-di(2-chloroethyl)-4-methyl-2,6-dinitroaniline]; nitralin [4-(methylsulfonyl)2,6-dinitro-N,N-dipropylaniline]; dinitramine(N4,N4-diethyl-α,α,α-trifluoro-3,5-dinitrotoluene-2,4-diamine); isopropalin (2,6-dinitro-N,N-dipropylcumidine), and profluralin [N(cyclopropylmethyl)-α,α,α-trifluoro-2,6-dinitro-N-propyl-p-toluidine]. The molar concentration required to inhibit fresh weight gain by 50% (I50) was determined by using linear regression analysis on data from a range of five concentrations of each chemical. I50values did not correlate with structural variations or available physical constants. Herbicides listed in order of increasing activity are AC 92390< GD-38946<profluralin = isopropalin<benefin = chlornidine = trifluralin = nitralin<oryzalin = penoxalin < dinitramine. Exogenously applied D-α-tocopherol acetate at 100 times the I50concentrations decreased the inhibition of tissue growth by the herbicides.

Morphogenesis in callus tissue cultures of someMatricaria andAchillea Species

1982 ◽  
Vol 24 (6) ◽  
pp. 430-433 ◽  
Author(s):  
Eva Čellárová ◽  
Klára Greláková ◽  
M. Repčák ◽  
R. Hončariv
Keyword(s):  
Callus Tissue ◽  
Tissue Cultures
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