The isolation of potato virus S from the leaves of potato plants using precipitation by polyethylene glycol

1968 ◽  
Vol 10 (4) ◽  
pp. 271-274 ◽  
Author(s):  
Liana Albrechtová ◽  
O. Klír
Keyword(s):  
Polyethylene Glycol ◽  
Potato Virus ◽  
Potato Virus S ◽  
Potato Plants

scholarly journals Monoclonal antibody-based serological assays for detection of Potato virus S in potato plants

2017 ◽  
Vol 18 (12) ◽  
pp. 1075-1082 ◽  
Author(s):  
Ge Song ◽  
Jia-yu Wu ◽  
Yan Xie ◽  
Yong Liu ◽  
Ya-juan Qian ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Potato Virus ◽  
Potato Virus S ◽  
Potato Plants

scholarly journals Tomato severe rugose virus: Another Begomovirus Causing Leaf Deformation and Mosaic Symptoms on Potato in Brazil

Plant Disease ◽  
2008 ◽  
Vol 92 (3) ◽  
pp. 487-487 ◽  
Author(s):  
J. A. C. Souza-Dias ◽  
H. E. Sawazaki ◽  
P. C. A. Pernambuco-Fo ◽  
L. M. Elias ◽  
H. Maluf
Keyword(s):  
Solanum Tuberosum ◽  
Seed Potato ◽  
Potato Virus ◽  
Mechanical Transmission ◽  
Streak Virus ◽  
Potato Leaf ◽  
Potato Virus S ◽  
Potato Plants ◽  
Datura Metel ◽  
Test Plants

Over the past 10 years, Tomato yellow vein streak virus (ToYVSV) has been a major begomovirus in the main solanaceous crop region of Campinas, São Paulo, which includes counties of Sumaré, Monte Mor, Elias Fausto, and Indaiatuba. The top leaves of potato plants (Solanum tuberosum) having deforming mosaic symptoms (dms), which includes a yellow mosaic or mottling on distorted and deformed leaflets, were associated with this geminivirus (4). Recently, a table potato crop (cv. Agata) from Sumaré, with a record of a few or no white flies (Bemisia tabaci), during the winter season of June to September 2006 had 5 to 7% dms, suggestive of seed potato tuber borne virus infection. Double-antibody sandwich (DAS)-ELISA for Potato virus Y (PVY), Potato leaf roll virus (PLRV), Potato virus X (PVX), and Potato virus S (PVS) (SASA kits and protocols, Edinburgh, Scotland) gave negative results for four field collected potato plants showing dms. Bioassays (mechanical transmission from potato leaf extracts in phosphate buffered saline, 1:5 w/v) with test plants of Nicotiana tabaccum cvs. Turkish and TNN, Gomphrena globosa, Chenopodium quinoa, Datura metel, Solanum tuberosum, and a Physalis sp. were all negative. Inoculated D. stramonium developed symptoms resembling ToYVSV infection including vein clearing and mild mottling on new leaves 2 to 3 weeks postinoculation. Using primers PAC1v1978/PAV1c715 for begomovirus detection (3), the predicted PCR amplified fragment of 1,320 bp was obtained from leaf DNA extracted from all four of the dms field potato plants, as well as the inoculated and symptomatic D. stramoniium test plants. Sequence analysis indicated 100% nt identity among the 1.3-kb PCR fragments obtained from potato and D. stramonium infected plants. Sequences of 96 cloned amplicons (pGEM-T Easy Kit; Promega, Madison, WI) from symptomatic plants in the Sumaré potato field were 98 to 99% identical to Tomato severe rugose virus (ToSRV). BLAST analysis of a consensus sequence (Sequencher 3.1; Gene Codes Corporation, Ann Arbor, MI) revealed more than 95 and 99% identity with ToSRV isolates from Uberlandia (Accession No. AY029750) and Goias (Accession No. DQ207749), respectively. The DNA-based phylogenetic dendrogram confirmed the highest similarity with ToSRV and the lowest similarity with ToYVSV (72%), which was located in another cluster. These results indicate that ToSRV was the causal agent producing dms in potato plants from Sumaré. Therefore, similarly to ToYVSV (4), potato dms can be caused by ToSRV. Preliminary tests revealed that ToSRV was transmitted via seed tubers. Thus, it is of concern for seed potato certification in Brazil, especially in the major seed-potato-producing state of Santa Catarina where an outbreak of ToSRV was recently reported in tomato crops (1). Although ToSRV has been identified in other solanaceous crops in Brazil, especially tomato (Lycopersicon esculentum) and sweet pepper (Capsicum annum) (2), to our knowledge, this is the first report of ToSRV in potato in Brazil. Reference: (1) A. T. M. Lima et al. Fitopatol. Bras. 31:224, 2006. (2) D. N. Nozaki et al. Summa Phytopathol. 33:93, 2007. (3) M. R. Rojas et al. Plant Dis. 77:340, 1993. (4) J. A. C. Souza-Dias et al. CultivarHF 5(26):22, 2004.

Elimination by chemotherapy of potato virus S from potato plants grown in vitro

1989 ◽  
Vol 32 (2) ◽  
pp. 175-179 ◽  
Author(s):  
Heide Bittner ◽  
G. Schenk ◽  
G. Schuster ◽  
S. Kluge
Keyword(s):  
Potato Virus ◽  
Potato Virus S ◽  
Potato Plants

Cytological responses of susceptible and extremely resistant potato plants to inoculation with potato virus Y

1999 ◽  
Vol 55 (3) ◽  
pp. 143-150 ◽  
Author(s):  
J. HINRICHS-BERGER ◽  
M. HARFOLD ◽  
S. BERGER ◽  
H. BUCHENAUER
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Plants

Detection of potato virus M and potato virus S on test plants

1976 ◽  
Vol 19 (2) ◽  
pp. 131-139 ◽  
Author(s):  
A. Kowalska ◽  
M. Waś
Keyword(s):  
Potato Virus ◽  
Potato Virus S ◽  
Potato Virus M ◽  
Test Plants

Spatiotemporal Spread of Potato virus S and Potato virus X in Seed Potato in Tasmania, Australia

2007 ◽  
Vol 8 (1) ◽  
pp. 70 ◽  
Author(s):  
Susan J. Lambert ◽  
Frank S. Hay ◽  
Sarah J. Pethybridge ◽  
Calum R. Wilson
Keyword(s):  
Seed Potato ◽  
Potato Virus ◽  
Temporal Distribution ◽  
Potato Virus X ◽  
Russet Burbank ◽  
Mechanical Transmission ◽  
Spatial And Temporal Distribution ◽  
Plant Spacing ◽  
Potato Virus S ◽  
The Mean

The spatial and temporal distribution of Potato virus S (PVS) and Potato virus X (PVX) was studied in two trials within each of four commercial fields of seed potato var. Russet Burbank in Tasmania, Australia. In the first trial (plots) 20 leaflets were collected from each of 49 plots (each approximately 8 m wide by 10 m long), with plots arranged in a 7-×-7 lattice. In the second trial (transects), leaflets were collected at 1-m intervals along seven adjacent, 50-m long rows. The mean incidence of PVS increased during the season by 5.2% in one of four plot trials and 25.5% in one of four transect trials. The mean incidence of PVX increased during the season by 10.1%, in one of two transect trials. Spatial Analysis by Distance IndicEs and ordinary runs analysis detected aggregation of PVS infected plants early in the season in one and two fields respectively, suggesting transmission during seed-cutting or during planting. An increase in PVS incidence mid- to late season in one field was associated with aggregation of PVS along, but not across rows, which may be related to the closer plant spacing within rows and hence increased potential for mechanical transmission along rows. Results suggested limited spread of PVS and PVX occurred within crops during the season. Accepted for publication 9 April 2007. Published 26 July 2007.

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