Elimination by chemotherapy of potato virus S from potato plants grown in vitro

1989 ◽  
Vol 32 (2) ◽  
pp. 175-179 ◽  
Author(s):  
Heide Bittner ◽  
G. Schenk ◽  
G. Schuster ◽  
S. Kluge
Keyword(s):  
Potato Virus ◽  
Potato Virus S ◽  
Potato Plants

Translation of potato virus S RNA in vitro: Evidence of protein processing

Virus Genes ◽  
1992 ◽  
Vol 6 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Gary D. Foster ◽  
Peter R. Mills
Keyword(s):  
Potato Virus ◽  
Protein Processing ◽  
Potato Virus S

Combined ribavirin treatment and cryotherapy for efficient Potato virus M and Potato virus S eradication in potato (Solanum tuberosum L.) in vitro shoots

2017 ◽  
Vol 53 (4) ◽  
pp. 425-432 ◽  
Author(s):  
Svetlana Kushnarenko ◽  
Natalya Romadanova ◽  
Moldir Aralbayeva ◽  
Saule Zholamanova ◽  
Alena Alexandrova ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Solanum Tuberosum L ◽  
Potato Virus S ◽  
In Vitro Shoots ◽  
Potato Virus M

scholarly journals Tissue Culture Methods for the Screening and Analysis of Putative Virus-Resistant Transgenic Potato Plants

1998 ◽  
Vol 88 (5) ◽  
pp. 437-441 ◽  
Author(s):  
P. Russo ◽  
S. A. Slack
Keyword(s):  
Tissue Culture ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Cultivar ◽  
Enzyme Linked Immunosorbent Assay ◽  
Insect Vector ◽  
Inoculation Methods ◽  
Potato Plants ◽  
Transgenic Lines

Following regeneration, putative virus-resistant transgenic plants are usually transferred from tissue culture to a greenhouse or growth chamber to screen for resistance to infection and disease development using mechanical, graft, or insect vector inoculation methods. To reduce initial screening costs and time, we developed mechanical and graft inoculation methods suitable for tissue culture use. The in vitro methods were validated by comparing them with similar greenhouse screens using putative potato virus Y strain o (PVY°) replicase-mediated resistant regenerants of the potato cultivar Atlantic. Five transgenic lines were tested, with similar results obtained from in vitro and greenhouse experiments. Two of the transgenic lines, A1 and A3, showed the greatest resistance to PVY°infection, as indicated by low enzyme-linked immunosorbent assay values and infection rates. In vitro mechanical inoculation methods were also used to infect wild-type tomato and tobacco plants with cucumber mosaic virus and potato virus Y. Potato plants were also infected with the phloem-restricted potato leafroll virus, a low-titer virus, using in vitro graft inoculation methods. These results suggest the potential usefulness of these simple, effective, and economical techniques for screening large numbers of putative virus-resistant plants.

scholarly journals Stock indexing and Potato virus Y elimination from potato plants cultivated in vitro

2003 ◽  
Vol 60 (3) ◽  
pp. 525-530 ◽  
Author(s):  
Luciana Cordeiro Nascimento ◽  
Gilvan Pio-Ribeiro ◽  
Lilia Willadino ◽  
Genira Pereira Andrade
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Solanum Tuberosum L ◽  
Potato Virus X ◽  
Naphthalene Acetic Acid ◽  
Virus Elimination ◽  
Single Node ◽  
Virus Eradication ◽  
Potato Virus S

Potato cultivars (Solanum tuberosum L.) have shown degeneration or run out caused by viruses after several cycles of propagation using seed tubers from commercial fields. This work reports the occurrence of single and mixed infections of four potato viruses in Paraíba-Brazil and presents a method for Potato virus Y (PVY) elimination, by using thermo-and chemotherapies. Plants of potato cv. Baraka were tested by direct antigen coating ELISA. Antisera against PVY, Potato virus X (PVX), Potato virus S (PVS), and Potato leafroll virus (PLRV) were used. Materials with positive reaction to PVY were treated for virus elimination. Single node cuttings (1.0 cm length) were excised and inoculated in Murashige & Skoog (MS) medium, supplemented with 1.0 mg L-1 of kinetin, 0.001 mg L-1 of naphthalene acetic acid (NAA) and 0.1 mg L-1 of gibberellic acid (GA3). The thermotherapy at approximately 37ºC, during 30 and 40 days, resulted in 20.0 and 37.5% PVY elimination, respectively. Chemotherapy was undertaken with Ribavirin (RBV), 5-Azacytidine (AZA), and 3-Deazauridine (DZD). The RBV showed the highest rate of virus eradication, with 55.5% virus-free plants. Simultaneous thermo and chemotherapy had higher efficiency for the elimination of PVY, reaching rates of healthy plants of 83.3% with RBV, 70.0% with AZA, and 50.0% with DZD.

scholarly journals Monoclonal antibody-based serological assays for detection of Potato virus S in potato plants

2017 ◽  
Vol 18 (12) ◽  
pp. 1075-1082 ◽  
Author(s):  
Ge Song ◽  
Jia-yu Wu ◽  
Yan Xie ◽  
Yong Liu ◽  
Ya-juan Qian ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Potato Virus ◽  
Potato Virus S ◽  
Potato Plants

scholarly journals Tomato severe rugose virus: Another Begomovirus Causing Leaf Deformation and Mosaic Symptoms on Potato in Brazil

Plant Disease ◽  
2008 ◽  
Vol 92 (3) ◽  
pp. 487-487 ◽  
Author(s):  
J. A. C. Souza-Dias ◽  
H. E. Sawazaki ◽  
P. C. A. Pernambuco-Fo ◽  
L. M. Elias ◽  
H. Maluf
Keyword(s):  
Solanum Tuberosum ◽  
Seed Potato ◽  
Potato Virus ◽  
Mechanical Transmission ◽  
Streak Virus ◽  
Potato Leaf ◽  
Potato Virus S ◽  
Potato Plants ◽  
Datura Metel ◽  
Test Plants

Over the past 10 years, Tomato yellow vein streak virus (ToYVSV) has been a major begomovirus in the main solanaceous crop region of Campinas, São Paulo, which includes counties of Sumaré, Monte Mor, Elias Fausto, and Indaiatuba. The top leaves of potato plants (Solanum tuberosum) having deforming mosaic symptoms (dms), which includes a yellow mosaic or mottling on distorted and deformed leaflets, were associated with this geminivirus (4). Recently, a table potato crop (cv. Agata) from Sumaré, with a record of a few or no white flies (Bemisia tabaci), during the winter season of June to September 2006 had 5 to 7% dms, suggestive of seed potato tuber borne virus infection. Double-antibody sandwich (DAS)-ELISA for Potato virus Y (PVY), Potato leaf roll virus (PLRV), Potato virus X (PVX), and Potato virus S (PVS) (SASA kits and protocols, Edinburgh, Scotland) gave negative results for four field collected potato plants showing dms. Bioassays (mechanical transmission from potato leaf extracts in phosphate buffered saline, 1:5 w/v) with test plants of Nicotiana tabaccum cvs. Turkish and TNN, Gomphrena globosa, Chenopodium quinoa, Datura metel, Solanum tuberosum, and a Physalis sp. were all negative. Inoculated D. stramonium developed symptoms resembling ToYVSV infection including vein clearing and mild mottling on new leaves 2 to 3 weeks postinoculation. Using primers PAC1v1978/PAV1c715 for begomovirus detection (3), the predicted PCR amplified fragment of 1,320 bp was obtained from leaf DNA extracted from all four of the dms field potato plants, as well as the inoculated and symptomatic D. stramoniium test plants. Sequence analysis indicated 100% nt identity among the 1.3-kb PCR fragments obtained from potato and D. stramonium infected plants. Sequences of 96 cloned amplicons (pGEM-T Easy Kit; Promega, Madison, WI) from symptomatic plants in the Sumaré potato field were 98 to 99% identical to Tomato severe rugose virus (ToSRV). BLAST analysis of a consensus sequence (Sequencher 3.1; Gene Codes Corporation, Ann Arbor, MI) revealed more than 95 and 99% identity with ToSRV isolates from Uberlandia (Accession No. AY029750) and Goias (Accession No. DQ207749), respectively. The DNA-based phylogenetic dendrogram confirmed the highest similarity with ToSRV and the lowest similarity with ToYVSV (72%), which was located in another cluster. These results indicate that ToSRV was the causal agent producing dms in potato plants from Sumaré. Therefore, similarly to ToYVSV (4), potato dms can be caused by ToSRV. Preliminary tests revealed that ToSRV was transmitted via seed tubers. Thus, it is of concern for seed potato certification in Brazil, especially in the major seed-potato-producing state of Santa Catarina where an outbreak of ToSRV was recently reported in tomato crops (1). Although ToSRV has been identified in other solanaceous crops in Brazil, especially tomato (Lycopersicon esculentum) and sweet pepper (Capsicum annum) (2), to our knowledge, this is the first report of ToSRV in potato in Brazil. Reference: (1) A. T. M. Lima et al. Fitopatol. Bras. 31:224, 2006. (2) D. N. Nozaki et al. Summa Phytopathol. 33:93, 2007. (3) M. R. Rojas et al. Plant Dis. 77:340, 1993. (4) J. A. C. Souza-Dias et al. CultivarHF 5(26):22, 2004.

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