Light and electron microscopic detection of lectin binding sites in normal and disordered human gastric tissues

1989 ◽  
Vol 9 (2) ◽  
pp. 74-80
Author(s):  
Shen Shi-liang ◽  
Hu Zong-yi ◽  
Xu Yu-hui ◽  
Wu Zhong-bi
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Electron Microscopic ◽  
Microscopic Detection ◽  
Lectin Binding Sites

scholarly journals Coloidal gold, ferritin and peroxidase as markers for electron microscopic double labeling lectin techniques.

1978 ◽  
Vol 26 (3) ◽  
pp. 163-169 ◽  
Author(s):  
J Roth ◽  
M Binder
Keyword(s):  
Particle Size ◽  
Cell Surface ◽  
Quantitative Evaluation ◽  
Binding Sites ◽  
Colloidal Gold ◽  
Lectin Binding ◽  
Electron Microscopic ◽  
Double Labeling ◽  
Optimal Amount ◽  
Lectin Binding Sites

Three markers, colloidal gold, ferritin and peroxidase, were checked for usefulness in double labeling of lectin-binding sites. The amount of various lectins for the stabilization of good sols of a different particle size was evaluated. Several lectin-gold complexes were prepared for electron microscopic labeling purposes, and the optimal amount of various lectins needed for stabilization of gold solutions of a different particle size was determined. The following combinations were investigated for their usefulness in labeling two different lectin-binding sites: lectin-gold and lectin-gold (different particle size), lectin-gold and lectin-ferritin, as well as lectin-ferritin and lectin-peroxidase. Of these combinations the latter did not give satisfactory results for double labeling. In all single and double labeling techniques with the above mentioned markers the quantitative evaluation of the number of lectin-binding sites is not feasible, but these techniques will be of considerable value for the investigation of the dynamics of different lectin-binding sites on the cell surface.

Lectin-binding sites in chick limb buds

1989 ◽  
Vol 27 ◽  
pp. 82
Author(s):  
M. Narita ◽  
K. Yamashita ◽  
M. Yasuda
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Limb Buds ◽  
Lectin Binding Sites

Ultrastructural demonstration of lectin binding sites in the Golgi apparatus of rat epiphyseal chondrocytes

1988 ◽  
Vol 89 (2) ◽  
pp. 177-184 ◽  
Author(s):  
A. Velasco ◽  
J. Hidalgo ◽  
M. M�ller ◽  
G. Garcia-Herdugo
Keyword(s):  
Golgi Apparatus ◽  
Binding Sites ◽  
Lectin Binding ◽  
Lectin Binding Sites

Lectin binding sites of the mouse ovary, intraovarian and ovulated ova

1984 ◽  
Vol 80 (6) ◽  
pp. 527-533 ◽  
Author(s):  
T. -C. Wu ◽  
M. -C. Lee ◽  
Y. -J. Wan ◽  
I. Damjanov
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Mouse Ovary ◽  
Lectin Binding Sites
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