A post-embedding avidin—biotin peroxidase system to demonstrate the light and electron microscopic localization of lectin binding sites in rat kidney tubules

1986 ◽  
Vol 18 (7) ◽  
pp. 371-379 ◽  
Author(s):  
Carolyn J. P. Jones ◽  
R. W. Stoddart
Keyword(s):  
Binding Sites ◽  
Rat Kidney ◽  
Lectin Binding ◽  
Kidney Tubules ◽  
Electron Microscopic ◽  
Microscopic Localization ◽  
Lectin Binding Sites ◽  
Electron Microscopic Localization

scholarly journals Coloidal gold, ferritin and peroxidase as markers for electron microscopic double labeling lectin techniques.

1978 ◽  
Vol 26 (3) ◽  
pp. 163-169 ◽  
Author(s):  
J Roth ◽  
M Binder
Keyword(s):  
Particle Size ◽  
Cell Surface ◽  
Quantitative Evaluation ◽  
Binding Sites ◽  
Colloidal Gold ◽  
Lectin Binding ◽  
Electron Microscopic ◽  
Double Labeling ◽  
Optimal Amount ◽  
Lectin Binding Sites

Three markers, colloidal gold, ferritin and peroxidase, were checked for usefulness in double labeling of lectin-binding sites. The amount of various lectins for the stabilization of good sols of a different particle size was evaluated. Several lectin-gold complexes were prepared for electron microscopic labeling purposes, and the optimal amount of various lectins needed for stabilization of gold solutions of a different particle size was determined. The following combinations were investigated for their usefulness in labeling two different lectin-binding sites: lectin-gold and lectin-gold (different particle size), lectin-gold and lectin-ferritin, as well as lectin-ferritin and lectin-peroxidase. Of these combinations the latter did not give satisfactory results for double labeling. In all single and double labeling techniques with the above mentioned markers the quantitative evaluation of the number of lectin-binding sites is not feasible, but these techniques will be of considerable value for the investigation of the dynamics of different lectin-binding sites on the cell surface.

scholarly journals Applications of immunocolloids in light microscopy. II. Demonstration of lectin-binding sites in paraffin sections by the use of lectin-gold or glycoprotein-gold complexes.

1983 ◽  
Vol 31 (4) ◽  
pp. 547-552 ◽  
Author(s):  
J Roth
Keyword(s):  
Binding Sites ◽  
Colloidal Gold ◽  
Rat Kidney ◽  
Lectin Binding ◽  
High Specificity ◽  
Step Method ◽  
Gold Complexes ◽  
Tissue Sections ◽  
One Step ◽  
Lectin Binding Sites

A new procedure is presented for the light microscopic demonstration of specific sugar sequences of oligosaccharides in glycoconjugates by lectins combined with the colloidal gold marker system. Tissue sections from aldehyde-fixed and paraffin embedded rat kidney were stained either in a one-step method with lectin directly bound to particles of colloidal gold or in a two-step method using non-labeled lectin and glycoprotein labeled with colloidal gold. In both methods the presence of lectin-binding sites in the tissue sections is revealed by the appearance of a red coloration that is due to the accumulation of gold particles. The high specificity of the technique is combined with a good sensitivity and resolution as demonstrated by a differential plasma membrane staining in renal epithelial cells. The lectin-gold or glycoprotein-gold complexes remain stable for months and produce a permanent nonbleaching staining.

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