scholarly journals Recombination in diploid vegetative cells of Saccharomyces cerevisiae

1980 ◽  
Vol 45 (4) ◽  
pp. 211-224 ◽  
Author(s):  
Herschel Roman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Vegetative Cells

scholarly journals A Role for MMS4 in the Processing of Recombination Intermediates During Meiosis in Saccharomyces cerevisiae

Genetics ◽  
2001 ◽  
Vol 159 (4) ◽  
pp. 1511-1525 ◽  
Author(s):  
Teresa de los Santos ◽  
Josef Loidl ◽  
Brittany Larkin ◽  
Nancy M Hollingsworth
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Biochemical Data ◽  
Dna Metabolism ◽  
Spore Viability ◽  
Vegetative Cells ◽  
Recombination Checkpoint

Abstract The MMS4 gene of Saccharomyces cerevisiae was originally identified due to its sensitivity to MMS in vegetative cells. Subsequent studies have confirmed a role for MMS4 in DNA metabolism of vegetative cells. In addition, mms4 diploids were observed to sporulate poorly. This work demonstrates that the mms4 sporulation defect is due to triggering of the meiotic recombination checkpoint. Genetic, physical, and cytological analyses suggest that MMS4 functions after the single end invasion step of meiotic recombination. In spo13 diploids, red1, but not mek1, is epistatic to mms4 for sporulation and spore viability, suggesting that MMS4 may be required only when homologs are capable of undergoing synapsis. MMS4 and MUS81 are in the same epistasis group for spore viability, consistent with biochemical data that show that the two proteins function in a complex. In contrast, MMS4 functions independently of MSH5 in the production of viable spores. We propose that MMS4 is required for the processing of specific recombination intermediates during meiosis.

Translocation of zinc from vacuole to nucleus during yeast meiosis

1983 ◽  
Vol 25 (5) ◽  
pp. 415-419 ◽  
Author(s):  
Carl A. Bilinski ◽  
John J. Miller
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Staining Procedure ◽  
Vegetative Cells ◽  
Nuclear Compartments ◽  
Polyphosphate Bodies ◽  
Yeast Meiosis ◽  
Zinc Translocation

A novel staining procedure employing the UV fluorochrome DAPI (4′,6-diamidino-2-phenylindole∙2HCl) and dithizone (diphenylthiocarbazone) was developed for microcytochemical determination of sites of zinc localization in Saccharomyces cerevisiae Hansen. In vegetative cells vacuolar polyphosphate bodies stained with dithizone, whereas in sporulating cells nucleoli and centriolar plaques were dithizone-positive. Hence, dithizone not only permitted localization of zinc but also indicated zinc translocation from vacuolar to nuclear compartments during differentiation from the vegetative to sporulated state.

A SEROLOGICAL COMPARISON OF VEGETATIVE CELL AND ASCUS WALLS AND THE SPORE COAT OF SACCHAROMYCES CEREVISIAE

1966 ◽  
Vol 12 (3) ◽  
pp. 485-488 ◽  
Author(s):  
I. J. Snider ◽  
J. J. Miller
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Structure ◽  
Vegetative Cell ◽  
Proteolytic Enzyme ◽  
Spore Coat ◽  
Vegetative Cells

Cross-agglutination tests with sera obtained by injection of vegetative cells, asci, and spores into rabbits revealed no immunological distinction between the walls of vegetative cells and asci, whereas the spore coats were found to be serologically distinct from cell and ascus walls. Treatment of vegetative cells and asci with periodate or a proteolytic enzyme before agglutination tests gave results which suggest that the critical antigen is a protein structure.

scholarly journals Identification of Domains Required for Developmentally Regulated SNARE Function in Saccharomyces cerevisiae

Genetics ◽  
2000 ◽  
Vol 155 (4) ◽  
pp. 1643-1655 ◽  
Author(s):  
Aaron M Neiman ◽  
Luba Katz ◽  
Patrick J Brennwald
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Snare Complex ◽  
Binding Studies ◽  
Developmentally Regulated ◽  
Vegetative Cells ◽  
Additional Layer ◽  
Prospore Membrane ◽  
N Terminus ◽  
Developmental Conditions

Abstract Saccharomyces cerevisiae cells contain two homologues of the mammalian t-SNARE protein SNAP-25, encoded by the SEC9 and SPO20 genes. Although both gene products participate in post-Golgi vesicle fusion events, they cannot substitute for one another; Sec9p is active primarily in vegetative cells while Spo20p functions only during sporulation. We have investigated the basis for the developmental stage-specific differences in the function of these two proteins. Localization of the other plasma membrane SNARE subunits, Ssop and Sncp, in sporulating cells suggests that these proteins act in conjunction with Spo20p in the formation of the prospore membrane. In vitro binding studies demonstrate that, like Sec9p, Spo20p binds specifically to the t-SNARE Sso1p and, once bound to Sso1p, can complex with the v-SNARE Snc2p. Therefore, Sec9p and Spo20p interact with the same binding partners, but developmental conditions appear to favor the assembly of complexes with Spo20p in sporulating cells. Analysis of chimeric Sec9p/Spo20p molecules indicates that regions in both the SNAP-25 domain and the unique N terminus of Spo20p are required for activity during sporulation. Additionally, the N terminus of Spo20p is inhibitory in vegetative cells. Deletion studies indicate that activation and inhibition are separable functions of the Spo20p N terminus. Our results reveal an additional layer of regulation of the SNARE complex, which is necessary only in sporulating cells.

Developmental changes in translatable RNA species associated with meiosis and spore formation in Saccharomyces cerevisiae

1984 ◽  
Vol 4 (4) ◽  
pp. 695-702
Author(s):  
E M Weir-Thompson ◽  
I W Dawes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcriptional Control ◽  
De Novo ◽  
Sporulation Medium ◽  
Two Dimensional Gel Electrophoresis ◽  
Vegetative Cells ◽  
Control Of Gene Expression ◽  
Mrna Species ◽  
Reticulocyte Lysate ◽  
Translatable Mrna

During Saccharomyces cerevisiae sporulation distinct changes in translatable mRNA species have been detected by two-dimensional gel electrophoresis of the polypeptides produced in a messenger-dependent, cell-free rabbit reticulocyte lysate primed with RNA prepared from a/alpha, a/a, and alpha/alpha isogenic diploids at different stages of sporulation. The availability of functional mRNA increased by about 25% during the first 4 h after transfer of either sporulating (a/alpha), or nonsporulating (a/a and alpha/alpha) diploids to sporulation medium. Thereafter functional mRNA decreased such that in the a/alpha strain after 24 h there was only about 50% of the amount in vegetative cells; a less marked decrease was observed in the a/a and alpha/alpha strains. Of 750 mRNA species detected, 43 underwent alterations only during sporulation in the a/alpha strain, whereas 36 changes were common to all three strains and one mRNA specific to alpha/alpha vegetative cells was detected. Only four of the sporulation-specific changes were due to the de novo appearance of translatable species, and two of these became predominant species of the total population. The majority of the specific changes were due to either permanent or transient increases in the concentration of individual mRNA species; 11 decreases were found. Changes were found at most stages of sporulation, although many occurred in either of two stages, one early (before 2 h) and the other later (between 6 and 8 h) when commitment to meiotic segregation was beginning. The results provide evidence for both quantitative and, to a lesser extent, qualitative transcriptional control of gene expression during sporulation.

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