Translocation of zinc from vacuole to nucleus during yeast meiosis

1983 ◽  
Vol 25 (5) ◽  
pp. 415-419 ◽  
Author(s):  
Carl A. Bilinski ◽  
John J. Miller
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Staining Procedure ◽  
Vegetative Cells ◽  
Nuclear Compartments ◽  
Polyphosphate Bodies ◽  
Yeast Meiosis ◽  
Zinc Translocation

A novel staining procedure employing the UV fluorochrome DAPI (4′,6-diamidino-2-phenylindole∙2HCl) and dithizone (diphenylthiocarbazone) was developed for microcytochemical determination of sites of zinc localization in Saccharomyces cerevisiae Hansen. In vegetative cells vacuolar polyphosphate bodies stained with dithizone, whereas in sporulating cells nucleoli and centriolar plaques were dithizone-positive. Hence, dithizone not only permitted localization of zinc but also indicated zinc translocation from vacuolar to nuclear compartments during differentiation from the vegetative to sporulated state.

scholarly journals Minisatellite Variants Generated in Yeast Meiosis Involve DNA Removal During Gene Conversion

Genetics ◽  
2000 ◽  
Vol 156 (1) ◽  
pp. 7-20 ◽  
Author(s):  
Alexander J R Bishop ◽  
Edward J Louis ◽  
Rhona H Borts
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Tetrad Analysis ◽  
Selection For ◽  
Novel Alleles ◽  
Yeast Meiosis ◽  
Gene Conversions

Abstract Two yeast minisatellite alleles were cloned and inserted into a genetically defined interval in Saccharomyces cerevisiae. Analysis of flanking markers in combination with sequencing allowed the determination of the meiotic events that produced minisatellites with altered lengths. Tetrad analysis revealed that gene conversions, deletions, or complex combinations of both were involved in producing minisatellite variants. Similar changes were obtained following selection for nearby gene conversions or crossovers among random spores. The largest class of events involving the minisatellite was a 3:1 segregation of parental-size alleles, a class that would have been missed in all previous studies of minisatellites. Comparison of the sequences of the parental and novel alleles revealed that DNA must have been removed from the recipient array while a newly synthesized copy of donor array sequences was inserted. The length of inserted sequences did not appear to be constrained by the length of DNA that was removed. In cases where one or both sides of the insertion could be determined, the insertion endpoints were consistent with the suggestion that the event was mediated by alignment of homologous stretches of donor/recipient DNA.

scholarly journals A Role for MMS4 in the Processing of Recombination Intermediates During Meiosis in Saccharomyces cerevisiae

Genetics ◽  
2001 ◽  
Vol 159 (4) ◽  
pp. 1511-1525 ◽  
Author(s):  
Teresa de los Santos ◽  
Josef Loidl ◽  
Brittany Larkin ◽  
Nancy M Hollingsworth
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Biochemical Data ◽  
Dna Metabolism ◽  
Spore Viability ◽  
Vegetative Cells ◽  
Recombination Checkpoint

Abstract The MMS4 gene of Saccharomyces cerevisiae was originally identified due to its sensitivity to MMS in vegetative cells. Subsequent studies have confirmed a role for MMS4 in DNA metabolism of vegetative cells. In addition, mms4 diploids were observed to sporulate poorly. This work demonstrates that the mms4 sporulation defect is due to triggering of the meiotic recombination checkpoint. Genetic, physical, and cytological analyses suggest that MMS4 functions after the single end invasion step of meiotic recombination. In spo13 diploids, red1, but not mek1, is epistatic to mms4 for sporulation and spore viability, suggesting that MMS4 may be required only when homologs are capable of undergoing synapsis. MMS4 and MUS81 are in the same epistasis group for spore viability, consistent with biochemical data that show that the two proteins function in a complex. In contrast, MMS4 functions independently of MSH5 in the production of viable spores. We propose that MMS4 is required for the processing of specific recombination intermediates during meiosis.

Recombination hot spot in the human beta-globin gene cluster: meiotic recombination of human DNA fragments in Saccharomyces cerevisiae

1985 ◽  
Vol 5 (8) ◽  
pp. 2029-2038
Author(s):  
D Treco ◽  
B Thomas ◽  
N Arnheim
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
High Frequency ◽  
Genetic Recombination ◽  
Hot Spot ◽  
Globin Gene ◽  
Beta Globin ◽  
Beta Globin Gene ◽  
Human Dna ◽  
Yeast Meiosis

We describe a novel system for the analysis of sequence-specific meiotic recombination in Saccharomyces cerevisiae. A comparison of three adjacent restriction fragments from the human beta-globin locus revealed that one of them, previously hypothesized to contain a relative hot spot for genetic recombination, engages in reciprocal exchange during yeast meiosis significantly more frequently than either of the other two fragments. Removal of the longest of four potential Z-DNA-forming regions from this fragment does not affect the high frequency of genetic recombination.

scholarly journals A glucan from active dry baker’s yeast (Saccharomyces cerevisiae): A chemical and enzymatic investigation of the structure

2003 ◽  
Vol 68 (11) ◽  
pp. 805-809 ◽  
Author(s):  
Dragan Zlatkovic ◽  
Dragica Jakovljevic ◽  
Djordje Zekovic ◽  
Miroslav Vrvic
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Optical Rotation ◽  
Linear Chain ◽  
Periodate Oxidation ◽  
Methylation Analysis ◽  
Yeast Saccharomyces Cerevisiae ◽  
New Approach ◽  
Main Structural Feature ◽  
Or Groups

The structure of a polysaccharide consisting of D-glucose isolated from the cell-wall of active dry baker?s yeast (Saccharomyces cerevisiae) was investigated by using methylation analysis, periodate oxidation, mass spectrometry, NMR spectroscopy, and enzymic hydrolysis, as a new approach in determination of structures. The main structural feature of the polysaccharide deduced on the basis of the obtained results is a linear chain of (1?3)-linked ?-D-glucopyranoses, a part of which is substituted through the positions O-6. The side units or groups are either a single D-glucopyranose or (1?3)-?-oligoglucosides, linked to the main chaing through (1?6)-glucosidic linkages. The low optical rotation as well as the 13C-NMR and FTIR spectra suggest that the glycosidic linkages are in the ?-D-configuration.

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