Studies on the possible influence of the so-calledwound healing reaction on DNA synthesis in organ culture

1977 ◽  
Vol 25 (1) ◽  
pp. 75-82
Author(s):  
L. J. van Bogaert ◽  
A. De Coninck ◽  
J. Vrebos ◽  
G. Verheecke
Keyword(s):  
Organ Culture ◽  
Dna Synthesis

Epidermal DNA synthesis in organ culture expiants

1979 ◽  
Vol 31 (1) ◽  
pp. 181-191 ◽  
Author(s):  
I. L. Hansteen ◽  
O. H. Iversen ◽  
S. B. Refsum
Keyword(s):  
Organ Culture ◽  
Dna Synthesis

Effect of Digoxin on DNA Synthesis and Cell Viability in Human Breast Tumour Tissue in Organ Culture

Chemotherapy ◽  
1983 ◽  
Vol 29 (5) ◽  
pp. 368-372 ◽  
Author(s):  
I.R. Falconer ◽  
A.M. Beresford ◽  
A. Jones ◽  
A.T. Vacek
Keyword(s):  
Organ Culture ◽  
Dna Synthesis ◽  
Cell Viability ◽  
Breast Tumour ◽  
Tumour Tissue ◽  
Human Breast ◽  
Human Breast Tumour ◽  
Breast Tumour Tissue

Effects of ACTH and ACTH fragments on DNA synthesis in guinea-pig adrenal segments kept in organ culture

1987 ◽  
Vol 115 (3) ◽  
pp. 505-510 ◽  
Author(s):  
N. M. Wulffraat ◽  
H. A. Drexhage ◽  
P. Jeucken ◽  
R. D. van der Gaag ◽  
W. M. Wiersinga
Keyword(s):  
Guinea Pig ◽  
Organ Culture ◽  
Dna Synthesis ◽  
Intermediate Lobe ◽  
Terminal Part ◽  
Terminal Fragment ◽  
Acth Fragments ◽  
Epidermal Growth ◽  
Stimulation Of

ABSTRACT Stimulation of adrenal DNA synthesis by ACTH(1–39) and its fragments ACTH(1–24) (Synacthen) and ACTH(18–39) was investigated. Synthesis of DNA was measured as the increase in the percentage of cells in S-phase (Feulgen densitometry) in guinea-pig adrenal explants kept in organ culture and exposed to the peptides for 5 h at 37 °C. ACTH(1–39) and its C-terminal fragment ACTH(18–39) (corticotrophin-like intermediate lobe peptide) were found to be potent stimulators of in-vitro adrenal DNA synthesis. The dose–response kinetics were biphasic and optimal responsiveness was reached in both instances at 1 fmol/1–10 pmol/l (this biological effect of ACTH(18–39) has hitherto not been described). The N-terminal fragment ACTH(1–24) gave only minimal responses. Thyrotrophin and LH, tested as controls, did not induce adrenal DNA synthesis. Epidermal growth factor was a potent stimulator of adrenal DNA synthesis in vitro. Our data suggest a trophic action of the C-terminal part (ACTH(18–39)) of the corticotrophic molecule. Clear trophic effects were also found for the N-terminal part of the pro-opiomelanocortin molecule N-POC(1–76) (optimum 0·1 nmol/l) and N-POC(51– 62) (optimum 0·1 pmol/l). The latter observations support earlier concepts that this part of the proopiomelanocortin molecule has a stimulatory effect on adrenal DNA synthesis. J. Endocr. (1987) 115, 505–510

DNA synthesis in adult rat anterior pituitary glands in organ culture

1969 ◽  
Vol 54 (3) ◽  
pp. 407-414 ◽  
Author(s):  
Andrea Mastro ◽  
W.C. Hymer ◽  
C.D. Therrien
Keyword(s):  
Organ Culture ◽  
Dna Synthesis ◽  
Anterior Pituitary ◽  
Adult Rat ◽  
Pituitary Glands

CORRELATION BETWEEN THE EFFECTS OF HORMONES ON THE SYNTHESIS OF DNA IN EXPLANTS FROM INDUCED RAT MAMMARY TUMOURS AND THE GROWTH OF THE TUMOURS

1974 ◽  
Vol 62 (2) ◽  
pp. 225-240 ◽  
Author(s):  
D. LEWIS ◽  
R. C. HALLOWES
Keyword(s):  
Organ Culture ◽  
Dna Synthesis ◽  
Culture Medium ◽  
Mammary Glands ◽  
Sprague Dawley ◽  
Culture Techniques ◽  
Mammary Tumours ◽  
Sprague Dawley Rats

SUMMARY Explants from 32 mammary tumours induced in Sprague—Dawley rats by 9,10-dimethyl-1,2-benzanthracene (DMBA) were maintained in organ culture for up to 48 h. Insulin, corticosterone, prolactin, growth hormone and oestradiol were added to the culture medium in various combinations and their effects on the DNA synthesis of the explants was studied. DNA synthesis was stimulated by insulin in explants from 30 out of the 32 tumours examined and this group of 30 responsive tumours could be further subdivided. Explants from 16 tumours showed a greater rate of DNA synthesis in medium containing insulin plus corticosterone plus prolactin than in medium containing insulin alone and this higher rate was decreased by oestradiol; this group is referred to as 'prolactin-responsive'. Explants from the remaining 14 tumours did not show a greater rate of DNA synthesis in medium that contained insulin plus corticosterone plus prolactin than in medium containing insulin alone and neither rate was decreased by oestradiol; this group is referred to as 'insulin-responsive'. Explants from two tumours were not stimulated by insulin and these tumours are referred to as 'non-responsive'. After oophorectomy or administration of ergocryptine to tumour-bearing rats, the prolactin-responsive tumours regressed whereas the non-responsive tumours continued to grow. Explants taken from prolactin-responsive tumours 2 weeks after either oophorectomy or administration of ergocryptine were still prolactin-responsive but those taken from insulin-responsive tumours 2 weeks after the same treatment were now also prolactin-responsive. The non-responsive tumours remained non-responsive. The effects of hormones on the DNA synthesis in vitro of explants from growing DMBA-induced tumours were thus different from those on explants of mammary glands from virgin or pregnant Sprague—Dawley rats. It was concluded that it was possible to predict by organ culture techniques the response in vivo of growing mammary tumours to oophorectomy and ergocryptine administration.

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