Effects of GAL10-SUC2 promoter combinations on SUC2 gene expression in S. cerevisiae

1993 ◽  
Vol 13 (2) ◽  
pp. 77-83
Author(s):  
Feng Bo ◽  
Li Yu-yang ◽  
Chen Zhao-cong
Keyword(s):  
Gene Expression ◽  
Suc2 Promoter ◽  
Suc2 Gene

Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccharomyces cerevisiae

1984 ◽  
Vol 4 (12) ◽  
pp. 2750-2757
Author(s):  
L Sarokin ◽  
M Carlson
Keyword(s):  
Gene Expression ◽  
Glucose Repression ◽  
Transcriptional Start Site ◽  
Upstream Region ◽  
Yeast Genome ◽  
Base Pairs ◽  
Regulated Expression ◽  
Suc2 Gene ◽  
High Level

The SUC2 gene produces two mRNAs with different 5' ends that encode two forms of invertase. The 1.9-kilobase mRNA encoding secreted invertase is regulated by glucose repression (carbon catabolite repression), and the 1.8-kilobase mRNA encoding intracellular invertase is produced constitutively at low levels. To identify 5' noncoding sequences essential for regulated expression of SUC2, we constructed in vitro a series of deletions and inserted them into the yeast genome at the chromosomal SUC2 locus. Analysis of the effects of each deletion on SUC2 gene expression identified an upstream region required for derepression of secreted invertase synthesis. The 3' boundary of this region is near -418. The 5' boundary does not appear to be sharply defined, but lies ca. 100 base pairs upstream. A deletion extending from -418 to -140 allowed high-level derepression, indicating that no essential sequences lie between the upstream region and the TATA box at -133 and that the upstream region can be moved 279 base pairs closer to the transcriptional start site. Interactions between the deletions and several unlinked mutations affecting the regulation of SUC2 gene expression were examined. Sequences between -1,900 and -86 are dispensable for expression of the 1.8-kilobase mRNA.

scholarly journals Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccharomyces cerevisiae.

1984 ◽  
Vol 4 (12) ◽  
pp. 2750-2757 ◽  
Author(s):  
L Sarokin ◽  
M Carlson
Keyword(s):  
Gene Expression ◽  
Glucose Repression ◽  
Transcriptional Start Site ◽  
Upstream Region ◽  
Yeast Genome ◽  
Base Pairs ◽  
Regulated Expression ◽  
Suc2 Gene ◽  
High Level

The SUC2 gene produces two mRNAs with different 5' ends that encode two forms of invertase. The 1.9-kilobase mRNA encoding secreted invertase is regulated by glucose repression (carbon catabolite repression), and the 1.8-kilobase mRNA encoding intracellular invertase is produced constitutively at low levels. To identify 5' noncoding sequences essential for regulated expression of SUC2, we constructed in vitro a series of deletions and inserted them into the yeast genome at the chromosomal SUC2 locus. Analysis of the effects of each deletion on SUC2 gene expression identified an upstream region required for derepression of secreted invertase synthesis. The 3' boundary of this region is near -418. The 5' boundary does not appear to be sharply defined, but lies ca. 100 base pairs upstream. A deletion extending from -418 to -140 allowed high-level derepression, indicating that no essential sequences lie between the upstream region and the TATA box at -133 and that the upstream region can be moved 279 base pairs closer to the transcriptional start site. Interactions between the deletions and several unlinked mutations affecting the regulation of SUC2 gene expression were examined. Sequences between -1,900 and -86 are dispensable for expression of the 1.8-kilobase mRNA.

scholarly journals Control of Gene Expression from the SUC2 Promoter of Saccharomyces cerevisiae using Two Carbon Sources.

1992 ◽  
Vol 18 (5) ◽  
pp. 693-700 ◽  
Author(s):  
Takayuki Ohshima ◽  
Xiao-Li Zhang ◽  
Shinji Iijima ◽  
Takeshi Kobayashi ◽  
Fumio Hishinuma
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Carbon Sources ◽  
Control Of Gene Expression ◽  
Suc2 Promoter

scholarly journals Analysis of cloned SUC2 gene expression in continuous culture of recombinantSaccharomyces cerevisiae

1990 ◽  
Vol 36 (9) ◽  
pp. 960-964 ◽  
Author(s):  
Jae K. Jang ◽  
Yu R. Pyun ◽  
Pyong K. Shin ◽  
Jin-Ho Seo
Keyword(s):  
Gene Expression ◽  
Continuous Culture ◽  
Suc2 Gene

Mutations in GCR1 affect SUC2 gene expression in Saccharomyces cerevisiae

2003 ◽  
Vol 268 (6) ◽  
pp. 825-831 ◽  
Author(s):  
S. Türkel ◽  
T. Turgut ◽  
M. C. López ◽  
H. Uemura ◽  
H. V. Baker
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Suc2 Gene

scholarly journals Two zinc-finger-containing repressors are responsible for glucose repression of SUC2 expression.

1996 ◽  
Vol 16 (9) ◽  
pp. 4790-4797 ◽  
Author(s):  
L L Lutfiyya ◽  
M Johnston
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Zinc Finger ◽  
Binding Sites ◽  
Glucose Repression ◽  
Zinc Fingers ◽  
Dependent Manner ◽  
Suc2 Promoter ◽  
Suc2 Gene

Expression of the SUC2 gene in Saccharomyces cerevisiae, which encodes invertase, is repressed about 200-fold by high levels of glucose. Mig1p is a Cys2His2 zinc-finger-containing protein required for glucose repression of SUC2 and several other genes. However, SUC2 expression is still about 13-fold repressed by glucose in a mig1 mutant. We have identified a second repressor, Mig2p, containing zinc fingers very similar to those of Mig1p that is responsible for this remaining glucose repression of SUC2 expression. Overexpression of MIG2 represses SUC2 under nonrepressing conditions, and a LexA-Mig2p fusion represses transcription of a lexO-containing promoter in a glucose-dependent manner, supporting the idea that Mig2p is a glucose-activated repressor. We have shown that Mig2p binds to the Miglp-binding sites in the SUC2 promoter. Even though Mig1p and Mig2p bind to similar sites and share almost identical zinc fingers, they differ in their relative affinities for various Mig1p-binding sites. This could explain our observation that MIG2 appears to have little role in glucose repression of other promoters with MIG1-binding sites.

GENES AFFECTING THE REGULATION OF SUC2 GENE EXPRESSION BY GLUCOSE REPRESSION IN SACCHAROMYCES CEREVISIAE

Genetics ◽  
1984 ◽  
Vol 108 (4) ◽  
pp. 845-858
Author(s):  
Lenore Neigeborn ◽  
Marian Carlson
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Glucose Repression ◽  
Invertase Activity ◽  
Recessive Mutations ◽  
Glycerol Utilization ◽  
Suc2 Gene ◽  
Complementation Groups ◽  
Low Levels ◽  
High Level

ABSTRACT Mutants of Saccharomyces cerevisiae with defects in sucrose or raffinose fermentation were isolated. In addition to mutations in the SUC2 structural gene for invertase, we recovered 18 recessive mutations that affected the regulation of invertase synthesis by glucose repression. These mutations included five new snf1 (sucrose nonfermenting) alleles and also defined five new complementation groups, designated snf2, snf3, snf4, snf5 and snf6. The snf2, snf4 and snf5 mutants produced little or no secreted invertase under derepressing conditions and were pleiotropically defective in galactose and glycerol utilization, which are both regulated by glucose repression. The snf6 mutant produced low levels of secreted invertase under derepressing conditions, and no pleiotropy was detected. The snf3 mutants derepressed secreted invertase to 10-35% the wild-type level but grew less well on sucrose than expected from their invertase activity; in addition, snf3 mutants synthesized some invertase under glucose-repressing conditions.—We examined the interactions between the different snf mutations and ssn6, a mutation causing constitutive (glucose-insensitive) high-level invertase synthesis that was previously isolated as a suppressor of snf1 . The ssn6 mutation completely suppressed the defects in derepression of invertase conferred by snf1, snf3, snf4 and snf6, and each double mutant showed the constitutivity for invertase typical of ssn6 single mutants. In contrast, snf2 ssn6 and snf5 ssn6 strains produced only moderate levels of invertase under derepressing conditions and very low levels under repressing conditions. These findings suggest roles for the SNF1 through SNF6 and SSN6 genes in the regulation of SUC2 gene expression by glucose repression.

Control of gene expression from the SUC2 promoter of Saccharomyces cerevisiae with the aid of a glucose analyser

1989 ◽  
Vol 32 (3) ◽  
Author(s):  
Kong Hua Lin ◽  
Shinji Iijima ◽  
Kazuyuki Shimizu ◽  
Fumio Hishinuma ◽  
Takeshi Kobayashi
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Control Of Gene Expression ◽  
Suc2 Promoter
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