scholarly journals Analysis of cloned SUC2 gene expression in continuous culture of recombinantSaccharomyces cerevisiae

1990 ◽  
Vol 36 (9) ◽  
pp. 960-964 ◽  
Author(s):  
Jae K. Jang ◽  
Yu R. Pyun ◽  
Pyong K. Shin ◽  
Jin-Ho Seo
Keyword(s):  
Gene Expression ◽  
Continuous Culture ◽  
Suc2 Gene

Effects of GAL10-SUC2 promoter combinations on SUC2 gene expression in S. cerevisiae

1993 ◽  
Vol 13 (2) ◽  
pp. 77-83
Author(s):  
Feng Bo ◽  
Li Yu-yang ◽  
Chen Zhao-cong
Keyword(s):  
Gene Expression ◽  
Suc2 Promoter ◽  
Suc2 Gene

Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccharomyces cerevisiae

1984 ◽  
Vol 4 (12) ◽  
pp. 2750-2757
Author(s):  
L Sarokin ◽  
M Carlson
Keyword(s):  
Gene Expression ◽  
Glucose Repression ◽  
Transcriptional Start Site ◽  
Upstream Region ◽  
Yeast Genome ◽  
Base Pairs ◽  
Regulated Expression ◽  
Suc2 Gene ◽  
High Level

The SUC2 gene produces two mRNAs with different 5' ends that encode two forms of invertase. The 1.9-kilobase mRNA encoding secreted invertase is regulated by glucose repression (carbon catabolite repression), and the 1.8-kilobase mRNA encoding intracellular invertase is produced constitutively at low levels. To identify 5' noncoding sequences essential for regulated expression of SUC2, we constructed in vitro a series of deletions and inserted them into the yeast genome at the chromosomal SUC2 locus. Analysis of the effects of each deletion on SUC2 gene expression identified an upstream region required for derepression of secreted invertase synthesis. The 3' boundary of this region is near -418. The 5' boundary does not appear to be sharply defined, but lies ca. 100 base pairs upstream. A deletion extending from -418 to -140 allowed high-level derepression, indicating that no essential sequences lie between the upstream region and the TATA box at -133 and that the upstream region can be moved 279 base pairs closer to the transcriptional start site. Interactions between the deletions and several unlinked mutations affecting the regulation of SUC2 gene expression were examined. Sequences between -1,900 and -86 are dispensable for expression of the 1.8-kilobase mRNA.

scholarly journals Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccharomyces cerevisiae.

1984 ◽  
Vol 4 (12) ◽  
pp. 2750-2757 ◽  
Author(s):  
L Sarokin ◽  
M Carlson
Keyword(s):  
Gene Expression ◽  
Glucose Repression ◽  
Transcriptional Start Site ◽  
Upstream Region ◽  
Yeast Genome ◽  
Base Pairs ◽  
Regulated Expression ◽  
Suc2 Gene ◽  
High Level

The SUC2 gene produces two mRNAs with different 5' ends that encode two forms of invertase. The 1.9-kilobase mRNA encoding secreted invertase is regulated by glucose repression (carbon catabolite repression), and the 1.8-kilobase mRNA encoding intracellular invertase is produced constitutively at low levels. To identify 5' noncoding sequences essential for regulated expression of SUC2, we constructed in vitro a series of deletions and inserted them into the yeast genome at the chromosomal SUC2 locus. Analysis of the effects of each deletion on SUC2 gene expression identified an upstream region required for derepression of secreted invertase synthesis. The 3' boundary of this region is near -418. The 5' boundary does not appear to be sharply defined, but lies ca. 100 base pairs upstream. A deletion extending from -418 to -140 allowed high-level derepression, indicating that no essential sequences lie between the upstream region and the TATA box at -133 and that the upstream region can be moved 279 base pairs closer to the transcriptional start site. Interactions between the deletions and several unlinked mutations affecting the regulation of SUC2 gene expression were examined. Sequences between -1,900 and -86 are dispensable for expression of the 1.8-kilobase mRNA.

scholarly journals Adherence and Cytokine Induction in Caco-2 Cells by Bacterial Populations from a Three-Stage Continuous-Culture Model of the Large Intestine

2011 ◽  
Vol 77 (9) ◽  
pp. 2934-2942 ◽  
Author(s):  
Bahram Bahrami ◽  
Matthew W. Child ◽  
Sandra Macfarlane ◽  
George T. Macfarlane
Keyword(s):  
Gene Expression ◽  
Continuous Culture ◽  
Large Intestine ◽  
Transforming Growth Factor ◽  
Bifidobacterium Longum ◽  
Cytokine Expression ◽  
Bacterial Attachment ◽  
Cytokine Gene Expression ◽  
Culture Vessel ◽  
Content Type

ABSTRACTAdherence of bacteria to epithelial cells is an important step in colonization and immune modulation in the large bowel. The aims of this study were to use a three-stage continuous-culture system (CCS) to investigate how environmental factors affect bacterial attachment to Caco-2 cells and modulation of cytokine expression by gut microorganisms, including a probioticBifidobacterium longumstrain, DD2004. The CCS simulated environmental conditions in the proximal large intestine (vessel 1 [V1]) and distal colon (V2 and V3) at two different system retention times (R) within the range of normal colonic transits (20 and 60 h). The model was inoculated with human fecal material, and fluorescencein situhybridization (FISH) was used to characterize microbial populations and to assess bacterial attachment to Caco-2 cells. Real-time quantitative PCR (qPCR) was employed to measure cytokine gene expression following challenge with bacteria from different components of the CCS in the presence and absence ofB. longum. At anRof 60 h, bacterial adherence increased from V1 to V3, but this trend was reversed at anRof 20 h. Atopobia were the predominant adherent organisms detected at both system retention times in each culture vessel. Modulation of transforming growth factor β1 (TGF-β1), interleukin 6 (IL-6), and IL-18 gene expression by CCS bacteria was marked at anRof 60 h, while at anRof 20 h, IL-4, IL-10, TGF-β2, IL-1α, and tumor necrosis factor alpha (TNF-α) were significantly affected. The addition ofB. longumaffected cytokine expression significantly at both retention times. This study demonstrates that environmental determinants regulate the adherence properties of intestinal bacteria and their abilities to regulate cytokine synthesis.

Mutations in GCR1 affect SUC2 gene expression in Saccharomyces cerevisiae

2003 ◽  
Vol 268 (6) ◽  
pp. 825-831 ◽  
Author(s):  
S. Türkel ◽  
T. Turgut ◽  
M. C. López ◽  
H. Uemura ◽  
H. V. Baker
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Suc2 Gene

scholarly journals Evaluation of Gene Expression and Alginate Production in Response to Oxygen Transfer in Continuous Culture of Azotobacter vinelandii

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e105993 ◽  
Author(s):  
Alvaro Díaz-Barrera ◽  
Fabiola Martínez ◽  
Felipe Guevara Pezoa ◽  
Fernando Acevedo
Keyword(s):  
Gene Expression ◽  
Continuous Culture ◽  
Oxygen Transfer ◽  
Azotobacter Vinelandii ◽  
Alginate Production

Gene expression of Escherichia coli in continuous culture during adaptation to artificial sunlight

2006 ◽  
Vol 8 (9) ◽  
pp. 1635-1647 ◽  
Author(s):  
Michael Berney ◽  
Hans-Ulrich Weilenmann ◽  
Thomas Egli
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Continuous Culture
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