Subzonal insemination with primary spermatocytes in mouse

1997 ◽  
Vol 42 (15) ◽  
pp. 1304-1308
Author(s):  
Ling Liu ◽  
Qingyuan Sun ◽  
Chongwen Duan ◽  
Hui Liu ◽  
Xiangfen Song ◽  
...  
Keyword(s):  
Subzonal Insemination

Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles

1993 ◽  
Vol 8 (7) ◽  
pp. 1055-1060 ◽  
Author(s):  
André C. Van Steirteghem ◽  
Jiaen Liu ◽  
Hubert Joris ◽  
Zsolt Nagy ◽  
Cécile Janssenswillen ◽  
...  
Keyword(s):  
Success Rate ◽  
Intracytoplasmic Sperm Injection ◽  
Subzonal Insemination

Assisted fertilization by subzonal insemination and intracytoplasmic sperm injection

1994 ◽  
Vol 6 (1) ◽  
pp. 85 ◽  
Author(s):  
Steirteghem A Van ◽  
J Liu ◽  
H Joris ◽  
Z Nagy ◽  
C Staessen ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Congenital Malformations ◽  
Subsequent Development ◽  
Fertilization Rate ◽  
Subzonal Insemination ◽  
Failed Fertilization ◽  
Assisted Fertilization ◽  
Normal Fertilization

The results of 600 consecutive treatment cycles of subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) are described in couples with failed fertilization after standard IVF or insufficient spermatozoa in the ejaculate for IVF. More oocytes were damaged by ICSI (16.3%) than by SUZI (8.5%) and the normal fertilization rate was substantially higher after ICSI (49.1% v. 16.6%). Subsequent development of two-pronuclear oocytes in vitro was 80% after SUZI and 73.9% after ICSI. Significantly more triple embryo replacements were carried out after ICSI than after SUZI. Embryo transfers were possible in 421 of the 600 cycles. There were 63 pregnancies after ICSI (215 transfers) and 23 after SUZI (156 transfers); 10 additional pregnancies were achieved after 50 transfers of a mixture of SUZI and ICSI embryos. The results of fetal karyotypes and follow-up of the children do not indicate an increase in congenital malformations.

Experience with subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) on unfertilized aged human oocytes

1994 ◽  
Vol 11 (8) ◽  
pp. 389-394 ◽  
Author(s):  
Marinos Tsirigotis ◽  
Valerie Bennett ◽  
Natasha Nicholson ◽  
Yasser Khalifa ◽  
Georg Hogewind ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Human Oocytes ◽  
Subzonal Insemination

Micro-assisted fertilization in patients who have failed subzonal insemination

1994 ◽  
Vol 9 (3) ◽  
pp. 501-505 ◽  
Author(s):  
Simon Fishel ◽  
Judy Timson ◽  
Franco Lisi ◽  
Merwyn Jacobson ◽  
Leonardo Rinaldi ◽  
...  
Keyword(s):  
Subzonal Insemination ◽  
Assisted Fertilization

Clinical intracytoplasmic sperm injection (ICSI) results from Royal North Shore Hospital

1995 ◽  
Vol 7 (2) ◽  
pp. 255 ◽  
Author(s):  
JW Catt ◽  
JP Ryan ◽  
IL Pike ◽  
C O'Neill ◽  
DM Saunders
Keyword(s):  
Embryo Transfer ◽  
Intracytoplasmic Sperm Injection ◽  
Ultrasound Examination ◽  
Controlled Study ◽  
Royal North Shore Hospital ◽  
North Shore ◽  
Intracytoplasmic Injection ◽  
Subzonal Insemination ◽  
Normal Fertilization ◽  
Fresh Transfer

The technique of intracytoplasmic sperm injection (ICSI) was first introduced to the Royal North Shore Hospital in April 1993 as part of a controlled study of 100 patient cycles in which sibling oocytes were inseminated by either subzonal insemination (SUZI) or ICSI. This trial showed direct sperm injection to be superior in terms of fertilization. In that study, 58 embryo transfers of 101 ICSI-derived embryos resulted in 10 pregnancies. No miscarriages have occurred and a total of 10 fetal hearts (9.8% per embryo transferred) were detected on ultrasound. There have been 10 deliveries of 10 babies. Since the beginning of 1994, intracytoplasmic injection has been used exclusively for patients requiring micromanipulation to achieve fertilization. There have been 200 patient cycles with 1650 oocytes collected (8.8 oocytes per cycle). Of these oocytes, 1548 were mature (94%) and were subjected to ICSI, and normal fertilization occurred in 874 (56%) of the injected oocytes. The number of oocytes which cleaved and were suitable for fresh transfer or cryopreservation was 818 (94%). There have been 153 fresh embryo transfers of 326 embryos. Twenty-six pregnancies (17% per embryo transfer) have resulted, 22 of which proceeded to ultrasound examination in which 23 fetal hearts were detected (7% per embryo transferred). Three miscarriages have occurred, leaving 19 ongoing pregnancies. There have been 127 cryopreservation procedures involving 492 embryos. To date, there have been 47 embryo thaw cycles, and 93 of the 115 (81%) thawed embryos survived and were transferred. These 47 embryo transfers resulted in 10 pregnancies (21% per embryo transfer), one of which one has miscarried.(ABSTRACT TRUNCATED AT 250 WORDS)

Subzonal insemination: A prospective randomized study in patients with abnormal sperm morphology

1994 ◽  
Vol 45 (2) ◽  
pp. 195-195
Author(s):  
S. Gordts ◽  
G. Garcia ◽  
M. Vercruyssen ◽  
P. Roziers ◽  
R. Campo ◽  
...  
Keyword(s):  
Sperm Morphology ◽  
Randomized Study ◽  
Prospective Randomized Study ◽  
Subzonal Insemination ◽  
Abnormal Sperm

Human oocyte cytometry and fertilisation rate after subzonal insemination

Zygote ◽  
1995 ◽  
Vol 3 (2) ◽  
pp. 101-109 ◽  
Author(s):  
Jean Philippe Wolf ◽  
Sylvie Bulwa ◽  
Daniel Rodrigues ◽  
Pierre Jouannet
Keyword(s):  
Zona Pellucida ◽  
Human Oocyte ◽  
Oocyte Diameter ◽  
Antisperm Antibodies ◽  
Subzonal Insemination ◽  
Fertilisation Rate ◽  
Defect Groups ◽  
The Mean ◽  
Dynein Arms

SummaryThe cytometry of 545 oocytes was evaluated during subzonal insemination (SUZI; 85 attempts), on day 0 (egg retrieval and SUZI), day 1 and day 2(embryo transfer). On day 0, the egg and oolemma diameters (mean ± SD) were 164.0 ± 19.6 μm and 114.2±16.8 μ5m respectively.The zona thickness was 17.8± 13.4 μm and correlated with the oolemma diameter(r = 0.24, p < 0.001). The fertilisation rate was significantly lower for the smaller oocytes (less than 108 μm diameter) compared with the larger oocytes (over 108μm) (9.8% vs 21.2% respectively; p < 0.05). These was little variation in oocyte diameter according to nuclear status. However, oocyte diameter increased significantly between day 0 and day 1 (p < 0.001) for both fertilised and unfertilised oocytes. Six different indications for SUZI were investigated in detail: three with non-specific (normal and subnormal sperm with in vitro fertilization failure, oligoasthenospermia) and three with specific sperm defects (flagellar dyskinesia, absence of outer dynein arms, antisperm antibodies). Oocytes from the non-specific defect groups had significantly smaller diameters than the others (p < 0.05). The mean fertilisation rate was related to the mean oolemma diameter for the groups with non-specific sperm defects and the group lacking dynein arms (LODA) (r = 0.91, p < 0.05). Eggs from the groups of patients with LODA and those with antisperm antibodies had thicker zona pellucida than others (p < 0.05). These findings suggest that in addition to nuclear criteria of maturity, the growth of oocytes is an important factor for fertilising ability. Insufficient development of the ooplasm may contribute to fertilisation failure, particularly when sperm with functional defects are used. In contrast, a thick zona pellucida may prevent sperm with specific anomalies such as LODA or antisperm antibodies from penetrating into the perivitelline space.

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